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[Ginkgo biloba extract enhances testosterone synthesis of Leydig cells in type 2 diabetic rats].
Zhonghua Nan Ke Xue. 2008 Apr; 14(4):371-6.ZN

Abstract

OBJECTIVE

To investigate the effects of Ginkgo biloba extract (EGB) on the testosterone synthesis in the Leydig cells of type 2 diabetic rats.

METHODS

Thirty male SD rats were equally randomised into a normal control, a type 2 diabetic and an EGB group. Morphological changes of Leydig cells were observed by light microscopy (LM) and transmission electron microscopy (TEM), concentrations of serum luteinizing hormone (LH) and testosterone (T) were determined by enzyme linked immunosorbent assay (ELISA), and the mRNA levels in the steroidogenic acute regulatory protein (StAR), cytochrome P450 side chain cleavage (P450scc), cytochrome P450 17a-hydroxylase (P450c17), 17beta-hydroxysteroid dehydrogenase 3 (17beta-HSD3) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD1) from the Leydig cells were examined by RT-PCR.

RESULTS

Compared with the normal control, there was a significant decrease in the number and volume of Leydig cells, the levels of serum LH and T and the expression of mRNA in StAR, P450scc, 17beta-HSD3 and 3beta-HSD1 in the type 2 diabetes group. And the expression of the P450c17 gene showed a tendency of descending, but with no significance. Compared with the type 2 diabetes group, 12 weeks of EGB treatment caused very slight pathological changes in the Leydig cells, significantly increased the concentrations of blood LH and T, markedly elevated the levels of mRNA in StAR and P450scc and induced an ascending tendency of the expressions of P450c17, 17beta-HSD3 and 3beta-HSD1.

CONCLUSION

EGB enhances testosterone synthesis and secretion of Leydig cells by reducing the impairment of the testis in type 2 diabetic rats.

Authors+Show Affiliations

Department of Pathology, 1st Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang 325000, China. xiaoyewu2005@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18481435

Citation

Wu, Xiao-Ye, et al. "[Ginkgo Biloba Extract Enhances Testosterone Synthesis of Leydig Cells in Type 2 Diabetic Rats]." Zhonghua Nan Ke Xue = National Journal of Andrology, vol. 14, no. 4, 2008, pp. 371-6.
Wu XY, Wang WY, Wang RR, et al. [Ginkgo biloba extract enhances testosterone synthesis of Leydig cells in type 2 diabetic rats]. Zhonghua Nan Ke Xue. 2008;14(4):371-6.
Wu, X. Y., Wang, W. Y., Wang, R. R., Xie, L., Fang, Z. X., & Chen, G. R. (2008). [Ginkgo biloba extract enhances testosterone synthesis of Leydig cells in type 2 diabetic rats]. Zhonghua Nan Ke Xue = National Journal of Andrology, 14(4), 371-6.
Wu XY, et al. [Ginkgo Biloba Extract Enhances Testosterone Synthesis of Leydig Cells in Type 2 Diabetic Rats]. Zhonghua Nan Ke Xue. 2008;14(4):371-6. PubMed PMID: 18481435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Ginkgo biloba extract enhances testosterone synthesis of Leydig cells in type 2 diabetic rats]. AU - Wu,Xiao-Ye, AU - Wang,Wen-Yan, AU - Wang,Rong-Rong, AU - Xie,Lin, AU - Fang,Zhou-Xi, AU - Chen,Guo-Rong, PY - 2008/5/17/pubmed PY - 2008/10/11/medline PY - 2008/5/17/entrez SP - 371 EP - 6 JF - Zhonghua nan ke xue = National journal of andrology JO - Zhonghua Nan Ke Xue VL - 14 IS - 4 N2 - OBJECTIVE: To investigate the effects of Ginkgo biloba extract (EGB) on the testosterone synthesis in the Leydig cells of type 2 diabetic rats. METHODS: Thirty male SD rats were equally randomised into a normal control, a type 2 diabetic and an EGB group. Morphological changes of Leydig cells were observed by light microscopy (LM) and transmission electron microscopy (TEM), concentrations of serum luteinizing hormone (LH) and testosterone (T) were determined by enzyme linked immunosorbent assay (ELISA), and the mRNA levels in the steroidogenic acute regulatory protein (StAR), cytochrome P450 side chain cleavage (P450scc), cytochrome P450 17a-hydroxylase (P450c17), 17beta-hydroxysteroid dehydrogenase 3 (17beta-HSD3) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD1) from the Leydig cells were examined by RT-PCR. RESULTS: Compared with the normal control, there was a significant decrease in the number and volume of Leydig cells, the levels of serum LH and T and the expression of mRNA in StAR, P450scc, 17beta-HSD3 and 3beta-HSD1 in the type 2 diabetes group. And the expression of the P450c17 gene showed a tendency of descending, but with no significance. Compared with the type 2 diabetes group, 12 weeks of EGB treatment caused very slight pathological changes in the Leydig cells, significantly increased the concentrations of blood LH and T, markedly elevated the levels of mRNA in StAR and P450scc and induced an ascending tendency of the expressions of P450c17, 17beta-HSD3 and 3beta-HSD1. CONCLUSION: EGB enhances testosterone synthesis and secretion of Leydig cells by reducing the impairment of the testis in type 2 diabetic rats. SN - 1009-3591 UR - https://www.unboundmedicine.com/medline/citation/18481435/[Ginkgo_biloba_extract_enhances_testosterone_synthesis_of_Leydig_cells_in_type_2_diabetic_rats]_ L2 - https://medlineplus.gov/diabetestype2.html DB - PRIME DP - Unbound Medicine ER -