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Antigen and antibody testing for the diagnosis of blastomycosis in dogs.
J Vet Intern Med. 2008 Jul-Aug; 22(4):839-43.JV

Abstract

BACKGROUND

Early diagnosis and treatment are associated with an improved prognosis in blastomycosis. The diagnosis of blastomycosis may be missed by cytology, histopathology, culture, or serology. An enzyme immunoassay (EIA) for detection of Blastomyces dermatitidis galactomannan antigen in body fluids has been used for rapid diagnosis of blastomycosis in humans.

HYPOTHESIS

Measurement of Blastomyces antigen in urine or serum by the MVista Blastomyces antigen EIA is more sensitive than measurement of anti-Blastomyces antibodies for diagnosis of blastomycosis in dogs.

METHODS

Serum and urine samples from 46 dogs with confirmed blastomycosis were tested for Blastomyces antigen and serum was tested for anti-Blastomyces antibodies.

RESULTS

The sensitivity for the detection of antigen in urine was 93.5% and it was 87.0% in serum. The sensitivity of antibody detection by agar gel immunodiffusion (AGID) was 17.4% and it was 76.1% by EIA. Antigen and antibody decreased during itraconazole treatment.

CONCLUSIONS AND CLINICAL IMPORTANCE

Antigen detection is a more sensitive test for diagnosis of blastomycosis than antibody testing by AGID, the only commercially available method. Antigen concentrations decreased with treatment.

Authors+Show Affiliations

Department of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18482276

Citation

Spector, D, et al. "Antigen and Antibody Testing for the Diagnosis of Blastomycosis in Dogs." Journal of Veterinary Internal Medicine, vol. 22, no. 4, 2008, pp. 839-43.
Spector D, Legendre AM, Wheat J, et al. Antigen and antibody testing for the diagnosis of blastomycosis in dogs. J Vet Intern Med. 2008;22(4):839-43.
Spector, D., Legendre, A. M., Wheat, J., Bemis, D., Rohrbach, B., Taboada, J., & Durkin, M. (2008). Antigen and antibody testing for the diagnosis of blastomycosis in dogs. Journal of Veterinary Internal Medicine, 22(4), 839-43. https://doi.org/10.1111/j.1939-1676.2008.0107.x
Spector D, et al. Antigen and Antibody Testing for the Diagnosis of Blastomycosis in Dogs. J Vet Intern Med. 2008 Jul-Aug;22(4):839-43. PubMed PMID: 18482276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen and antibody testing for the diagnosis of blastomycosis in dogs. AU - Spector,D, AU - Legendre,A M, AU - Wheat,J, AU - Bemis,D, AU - Rohrbach,B, AU - Taboada,J, AU - Durkin,M, PY - 2008/5/17/pubmed PY - 2008/10/29/medline PY - 2008/5/17/entrez SP - 839 EP - 43 JF - Journal of veterinary internal medicine JO - J Vet Intern Med VL - 22 IS - 4 N2 - BACKGROUND: Early diagnosis and treatment are associated with an improved prognosis in blastomycosis. The diagnosis of blastomycosis may be missed by cytology, histopathology, culture, or serology. An enzyme immunoassay (EIA) for detection of Blastomyces dermatitidis galactomannan antigen in body fluids has been used for rapid diagnosis of blastomycosis in humans. HYPOTHESIS: Measurement of Blastomyces antigen in urine or serum by the MVista Blastomyces antigen EIA is more sensitive than measurement of anti-Blastomyces antibodies for diagnosis of blastomycosis in dogs. METHODS: Serum and urine samples from 46 dogs with confirmed blastomycosis were tested for Blastomyces antigen and serum was tested for anti-Blastomyces antibodies. RESULTS: The sensitivity for the detection of antigen in urine was 93.5% and it was 87.0% in serum. The sensitivity of antibody detection by agar gel immunodiffusion (AGID) was 17.4% and it was 76.1% by EIA. Antigen and antibody decreased during itraconazole treatment. CONCLUSIONS AND CLINICAL IMPORTANCE: Antigen detection is a more sensitive test for diagnosis of blastomycosis than antibody testing by AGID, the only commercially available method. Antigen concentrations decreased with treatment. SN - 0891-6640 UR - https://www.unboundmedicine.com/medline/citation/18482276/Antigen_and_antibody_testing_for_the_diagnosis_of_blastomycosis_in_dogs_ L2 - https://doi.org/10.1111/j.1939-1676.2008.0107.x DB - PRIME DP - Unbound Medicine ER -