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Nuclear localization of Rad52 is pre-requisite for its sumoylation.
Biochem Biophys Res Commun. 2008 Jul 18; 372(1):126-30.BB

Abstract

In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation.

Authors+Show Affiliations

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18482582

Citation

Ohuchi, Takashi, et al. "Nuclear Localization of Rad52 Is Pre-requisite for Its Sumoylation." Biochemical and Biophysical Research Communications, vol. 372, no. 1, 2008, pp. 126-30.
Ohuchi T, Seki M, Enomoto T. Nuclear localization of Rad52 is pre-requisite for its sumoylation. Biochem Biophys Res Commun. 2008;372(1):126-30.
Ohuchi, T., Seki, M., & Enomoto, T. (2008). Nuclear localization of Rad52 is pre-requisite for its sumoylation. Biochemical and Biophysical Research Communications, 372(1), 126-30. https://doi.org/10.1016/j.bbrc.2008.05.020
Ohuchi T, Seki M, Enomoto T. Nuclear Localization of Rad52 Is Pre-requisite for Its Sumoylation. Biochem Biophys Res Commun. 2008 Jul 18;372(1):126-30. PubMed PMID: 18482582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear localization of Rad52 is pre-requisite for its sumoylation. AU - Ohuchi,Takashi, AU - Seki,Masayuki, AU - Enomoto,Takemi, Y1 - 2008/05/13/ PY - 2008/04/30/received PY - 2008/05/01/accepted PY - 2008/5/17/pubmed PY - 2008/6/21/medline PY - 2008/5/17/entrez SP - 126 EP - 30 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 372 IS - 1 N2 - In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18482582/Nuclear_localization_of_Rad52_is_pre_requisite_for_its_sumoylation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)00881-4 DB - PRIME DP - Unbound Medicine ER -