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AFM nanometer surface morphological study of in situ electropolymerized neutral red redox mediator oxysilane sol-gel encapsulated glucose oxidase electrochemical biosensors.
Biosens Bioelectron. 2008 Oct 15; 24(2):297-305.BB

Abstract

Four different silica sol-gel films: methyltrimethoxysilane (MTMOS), tetraethoxysilane (TEOS), 3-aminopropyltriethoxysilane (APTOS) and 3-glycidoxypropyl-trimethoxysilane (GOPMOS) assembled onto highly oriented pyrolytic graphite (HOPG) were characterized using atomic force microscopy (AFM), due to their use in the development of glucose biosensors. The chemical structure of the oxysilane precursor and the composition of the sol-gel mixture both influenced the roughness, the size and the distribution of pores in the sol-gel films, which is relevant for enzyme encapsulation. The GOPMOS sol-gel film fulfils all the morphological characteristics required for good encapsulation of the enzyme, due to a smooth topography with very dense and uniform distribution of only small, 50 nm diameter, pores at the surface. APTOS and MTMOS sol-gel films developed small pores together with large ones of 300-400 nm that allow the leakage of enzymes, while the TEOS film formed a rough and incomplete network on the electrode, less suitable for enzyme immobilisation. GOPMOS sol-gel film with encapsulated glucose oxidase and poly(neutral red) redox mediator, prepared by in situ electropolymerization, were also morphologically characterized by AFM. The AFM results explain the variation of the stability in time, sensitivity and limit of detection obtained with different oxysilane sol-gel encapsulated glucose oxidase biosensors with redox mediator.

Authors+Show Affiliations

Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Rua Larga. 3004-535 Coimbra, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18485690

Citation

Chiorcea-Paquim, Ana-Maria, et al. "AFM Nanometer Surface Morphological Study of in Situ Electropolymerized Neutral Red Redox Mediator Oxysilane Sol-gel Encapsulated Glucose Oxidase Electrochemical Biosensors." Biosensors & Bioelectronics, vol. 24, no. 2, 2008, pp. 297-305.
Chiorcea-Paquim AM, Pauliukaite R, Brett CM, et al. AFM nanometer surface morphological study of in situ electropolymerized neutral red redox mediator oxysilane sol-gel encapsulated glucose oxidase electrochemical biosensors. Biosens Bioelectron. 2008;24(2):297-305.
Chiorcea-Paquim, A. M., Pauliukaite, R., Brett, C. M., & Oliveira-Brett, A. M. (2008). AFM nanometer surface morphological study of in situ electropolymerized neutral red redox mediator oxysilane sol-gel encapsulated glucose oxidase electrochemical biosensors. Biosensors & Bioelectronics, 24(2), 297-305. https://doi.org/10.1016/j.bios.2008.04.001
Chiorcea-Paquim AM, et al. AFM Nanometer Surface Morphological Study of in Situ Electropolymerized Neutral Red Redox Mediator Oxysilane Sol-gel Encapsulated Glucose Oxidase Electrochemical Biosensors. Biosens Bioelectron. 2008 Oct 15;24(2):297-305. PubMed PMID: 18485690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AFM nanometer surface morphological study of in situ electropolymerized neutral red redox mediator oxysilane sol-gel encapsulated glucose oxidase electrochemical biosensors. AU - Chiorcea-Paquim,Ana-Maria, AU - Pauliukaite,Rasa, AU - Brett,Christopher M A, AU - Oliveira-Brett,Ana Maria, Y1 - 2008/04/11/ PY - 2008/02/12/received PY - 2008/04/01/revised PY - 2008/04/02/accepted PY - 2008/5/20/pubmed PY - 2008/11/19/medline PY - 2008/5/20/entrez SP - 297 EP - 305 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 2 N2 - Four different silica sol-gel films: methyltrimethoxysilane (MTMOS), tetraethoxysilane (TEOS), 3-aminopropyltriethoxysilane (APTOS) and 3-glycidoxypropyl-trimethoxysilane (GOPMOS) assembled onto highly oriented pyrolytic graphite (HOPG) were characterized using atomic force microscopy (AFM), due to their use in the development of glucose biosensors. The chemical structure of the oxysilane precursor and the composition of the sol-gel mixture both influenced the roughness, the size and the distribution of pores in the sol-gel films, which is relevant for enzyme encapsulation. The GOPMOS sol-gel film fulfils all the morphological characteristics required for good encapsulation of the enzyme, due to a smooth topography with very dense and uniform distribution of only small, 50 nm diameter, pores at the surface. APTOS and MTMOS sol-gel films developed small pores together with large ones of 300-400 nm that allow the leakage of enzymes, while the TEOS film formed a rough and incomplete network on the electrode, less suitable for enzyme immobilisation. GOPMOS sol-gel film with encapsulated glucose oxidase and poly(neutral red) redox mediator, prepared by in situ electropolymerization, were also morphologically characterized by AFM. The AFM results explain the variation of the stability in time, sensitivity and limit of detection obtained with different oxysilane sol-gel encapsulated glucose oxidase biosensors with redox mediator. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18485690/AFM_nanometer_surface_morphological_study_of_in_situ_electropolymerized_neutral_red_redox_mediator_oxysilane_sol_gel_encapsulated_glucose_oxidase_electrochemical_biosensors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00189-9 DB - PRIME DP - Unbound Medicine ER -