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DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label.
Biosens Bioelectron. 2008 Oct 15; 24(2):216-21.BB

Abstract

A technique is demonstrated to detect DNA hybridization at low concentrations, based on Surface-Enhanced Raman Scattering (SERS) using silicon nanostructures coated with gold-silver as substrate. Standard silicon process technologies were employed to fabricate the SERS substrates featuring nanogaps with a characteristic distance of 15+/-10nm. Target DNA was hybridized with cysteine-modified Peptide Nucleic Acids (PNA), which was previously fixed into the nanogaps as the capture sites. After hybridization, the introduced phosphate groups from the backbone of the target DNA showed strong affinity to an inorganic linker, Zr(4+), so that resulting in the assembly substrate-PNA-DNA-Zr. Since PNA does not possess phosphate groups, the linker is avoided when there is no hybridization from the complimentary DNA. Subsequently, the assembly of substrate-PNA-DNA-Zr was incubated with a Raman label, Rhodamine B (RB). The carboxylic acid group in RB reacted with the linker Zr(4+) allowing this Raman Label to be attached to the assembly substrate-PNA-DNA-Zr. The Raman peaks corresponding to RB were selected to detect the target DNA, with a detection limit of 1 x 10(-12)M.

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Authors+Show Affiliations

Fang C
Institute of Microelectronics, A STAR (Agency for Science, Technology and Research), Singapore Science Park II, Singapore. fangc@ime.a-star.edu.sg
Agarwal A
No affiliation info available
Buddharaju KD
No affiliation info available
Khalid NM
No affiliation info available
Salim SM
No affiliation info available
Widjaja E
No affiliation info available
Garland MV
No affiliation info available
Balasubramanian N
No affiliation info available
Kwong DL
No affiliation info available

MeSH

Biosensing TechniquesDNAEquipment DesignEquipment Failure AnalysisNanostructuresNanotechnologyReproducibility of ResultsRhodaminesSensitivity and SpecificitySpectrum Analysis, RamanStaining and Labeling

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18485693

Citation

Fang, Cheng, et al. "DNA Detection Using Nanostructured SERS Substrates With Rhodamine B as Raman Label." Biosensors & Bioelectronics, vol. 24, no. 2, 2008, pp. 216-21.
Fang C, Agarwal A, Buddharaju KD, et al. DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label. Biosens Bioelectron. 2008;24(2):216-21.
Fang, C., Agarwal, A., Buddharaju, K. D., Khalid, N. M., Salim, S. M., Widjaja, E., Garland, M. V., Balasubramanian, N., & Kwong, D. L. (2008). DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label. Biosensors & Bioelectronics, 24(2), 216-21. https://doi.org/10.1016/j.bios.2008.03.032
Fang C, et al. DNA Detection Using Nanostructured SERS Substrates With Rhodamine B as Raman Label. Biosens Bioelectron. 2008 Oct 15;24(2):216-21. PubMed PMID: 18485693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA detection using nanostructured SERS substrates with Rhodamine B as Raman label. AU - Fang,Cheng, AU - Agarwal,Ajay, AU - Buddharaju,Kavitha Devi, AU - Khalid,Nizamudin Mohamed, AU - Salim,Shaik Mohamed, AU - Widjaja,Effendi, AU - Garland,Marc V, AU - Balasubramanian,Narayanan, AU - Kwong,Dim-Lee, Y1 - 2008/04/04/ PY - 2007/12/27/received PY - 2008/03/07/revised PY - 2008/03/25/accepted PY - 2008/5/20/pubmed PY - 2008/11/19/medline PY - 2008/5/20/entrez SP - 216 EP - 21 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 2 N2 - A technique is demonstrated to detect DNA hybridization at low concentrations, based on Surface-Enhanced Raman Scattering (SERS) using silicon nanostructures coated with gold-silver as substrate. Standard silicon process technologies were employed to fabricate the SERS substrates featuring nanogaps with a characteristic distance of 15+/-10nm. Target DNA was hybridized with cysteine-modified Peptide Nucleic Acids (PNA), which was previously fixed into the nanogaps as the capture sites. After hybridization, the introduced phosphate groups from the backbone of the target DNA showed strong affinity to an inorganic linker, Zr(4+), so that resulting in the assembly substrate-PNA-DNA-Zr. Since PNA does not possess phosphate groups, the linker is avoided when there is no hybridization from the complimentary DNA. Subsequently, the assembly of substrate-PNA-DNA-Zr was incubated with a Raman label, Rhodamine B (RB). The carboxylic acid group in RB reacted with the linker Zr(4+) allowing this Raman Label to be attached to the assembly substrate-PNA-DNA-Zr. The Raman peaks corresponding to RB were selected to detect the target DNA, with a detection limit of 1 x 10(-12)M. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18485693/DNA_detection_using_nanostructured_SERS_substrates_with_Rhodamine_B_as_Raman_label_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00154-1 DB - PRIME DP - Unbound Medicine ER -