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Quantitative determination of mitiglinide in human plasma by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 01; 868(1-2):83-7.JC

Abstract

A selective, rapid and sensitive ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) method was developed for the quantitative determination of mitiglinide in human plasma. With nateglinide as internal standard, sample pretreatment involved a one-step extraction with diethyl ether of 0.2 mL plasma. The separation was performed on an ACQUITY UPLCtrade mark BEH C(18) column (50 mm x 2.1 mm, i.d., 1.7 microm) with the mobile phase consisting of methanol and 10 mmol/L ammonium acetate (65:35, v/v) at a flow rate of 0.25 mL/min. The detection was carried out by means of electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring (MRM). Linear calibration curves were obtained in the concentration range of 1.080-5400 ng/mL, with a lower limit of quantification of 1.080 ng/mL. The intra- and inter-day precision (RSD) values were below 15% and accuracy (RE) was from -3.5% to 7.3% at all QC levels. The method was fully validated and successfully applied to a clinical pharmacokinetic study of mitiglinide in 10 healthy volunteers following oral administration.

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Authors+Show Affiliations

Cai S
Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, PR China.
Huo T
No affiliation info available
Feng W
No affiliation info available
Chen L
No affiliation info available
Qin F
No affiliation info available
Li F
No affiliation info available

MeSH

CalibrationChromatography, LiquidHumansHypoglycemic AgentsIsoindolesReference StandardsTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18486570

Citation

Cai, Shuang, et al. "Quantitative Determination of Mitiglinide in Human Plasma By Ultra-performance Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 868, no. 1-2, 2008, pp. 83-7.
Cai S, Huo T, Feng W, et al. Quantitative determination of mitiglinide in human plasma by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;868(1-2):83-7.
Cai, S., Huo, T., Feng, W., Chen, L., Qin, F., & Li, F. (2008). Quantitative determination of mitiglinide in human plasma by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 868(1-2), 83-7. https://doi.org/10.1016/j.jchromb.2008.04.037
Cai S, et al. Quantitative Determination of Mitiglinide in Human Plasma By Ultra-performance Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 1;868(1-2):83-7. PubMed PMID: 18486570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination of mitiglinide in human plasma by ultra-performance liquid chromatography/electrospray ionization tandem mass spectrometry. AU - Cai,Shuang, AU - Huo,Taoguang, AU - Feng,Wanyu, AU - Chen,Lingyun, AU - Qin,Feng, AU - Li,Famei, Y1 - 2008/05/03/ PY - 2007/10/23/received PY - 2008/04/17/revised PY - 2008/04/24/accepted PY - 2008/5/20/pubmed PY - 2008/8/19/medline PY - 2008/5/20/entrez SP - 83 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 868 IS - 1-2 N2 - A selective, rapid and sensitive ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) method was developed for the quantitative determination of mitiglinide in human plasma. With nateglinide as internal standard, sample pretreatment involved a one-step extraction with diethyl ether of 0.2 mL plasma. The separation was performed on an ACQUITY UPLCtrade mark BEH C(18) column (50 mm x 2.1 mm, i.d., 1.7 microm) with the mobile phase consisting of methanol and 10 mmol/L ammonium acetate (65:35, v/v) at a flow rate of 0.25 mL/min. The detection was carried out by means of electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring (MRM). Linear calibration curves were obtained in the concentration range of 1.080-5400 ng/mL, with a lower limit of quantification of 1.080 ng/mL. The intra- and inter-day precision (RSD) values were below 15% and accuracy (RE) was from -3.5% to 7.3% at all QC levels. The method was fully validated and successfully applied to a clinical pharmacokinetic study of mitiglinide in 10 healthy volunteers following oral administration. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18486570/Quantitative_determination_of_mitiglinide_in_human_plasma_by_ultra_performance_liquid_chromatography/electrospray_ionization_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00289-4 DB - PRIME DP - Unbound Medicine ER -
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