Tags

Type your tag names separated by a space and hit enter

Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice.
BMC Cell Biol. 2008 May 19; 9:24.BC

Abstract

BACKGROUND

Human mesenchymal stem cells (MSCs) have been studied and applied extensively because of their ability to self-renew and differentiate into various cell types. Since most human diseases models are murine, mouse MSCs should have been studied in detail. The mdx mouse - a Duchenne muscular dystrophy model - was produced by introducing a point mutation in the dystrophin gene. To understand the role of dystrophin in MSCs, we compared MSCs from mdx and C57BL/10 mice, focusing particularly on the aspects of light and electron microscopic morphology, immunophenotyping, and differentiation potential.

RESULTS

Our study showed that at passage 10, mdx-MSCs exhibited increased heterochromatin, larger vacuoles, and more lysosomes under electron microscopy compared to C57BL/10-MSCs. C57BL/10-MSCs formed a few myotubes, while mdx-MSCs did not at the same passages. By passage 21, mdx-MSCs but not C57BL/10-MSCs had gradually lost their proliferative ability. In addition, a significant difference in the expression of CD34, not Sca-1 and CD11b, was observed between the MSCs from the 2 mice.

CONCLUSION

Our current study reveals that the MSCs from the 2 mice, namely, C57BL/10 and mdx, exhibit differences in proliferative and myogenic abilities. The results suggest that the changes in mouse MSC behavior may be influenced by lack of dystrophin protein in mdx mouse.

Authors+Show Affiliations

Department of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ProC. liyong999@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18489762

Citation

Li, Yong, et al. "Comparative Study of Mesenchymal Stem Cells From C57BL/10 and Mdx Mice." BMC Cell Biology, vol. 9, 2008, p. 24.
Li Y, Zhang C, Xiong F, et al. Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice. BMC Cell Biol. 2008;9:24.
Li, Y., Zhang, C., Xiong, F., Yu, M. J., Peng, F. L., Shang, Y. C., Zhao, C. P., Xu, Y. F., Liu, Z. S., Zhou, C., & Wu, J. L. (2008). Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice. BMC Cell Biology, 9, 24. https://doi.org/10.1186/1471-2121-9-24
Li Y, et al. Comparative Study of Mesenchymal Stem Cells From C57BL/10 and Mdx Mice. BMC Cell Biol. 2008 May 19;9:24. PubMed PMID: 18489762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice. AU - Li,Yong, AU - Zhang,Cheng, AU - Xiong,Fu, AU - Yu,Mei-juan, AU - Peng,Fu-Lin, AU - Shang,Yan-chang, AU - Zhao,Cui-ping, AU - Xu,Yong-feng, AU - Liu,Zheng-shan, AU - Zhou,Chang, AU - Wu,Jin-lang, Y1 - 2008/05/19/ PY - 2007/06/28/received PY - 2008/05/19/accepted PY - 2008/5/21/pubmed PY - 2009/3/7/medline PY - 2008/5/21/entrez SP - 24 EP - 24 JF - BMC cell biology JO - BMC Cell Biol VL - 9 N2 - BACKGROUND: Human mesenchymal stem cells (MSCs) have been studied and applied extensively because of their ability to self-renew and differentiate into various cell types. Since most human diseases models are murine, mouse MSCs should have been studied in detail. The mdx mouse - a Duchenne muscular dystrophy model - was produced by introducing a point mutation in the dystrophin gene. To understand the role of dystrophin in MSCs, we compared MSCs from mdx and C57BL/10 mice, focusing particularly on the aspects of light and electron microscopic morphology, immunophenotyping, and differentiation potential. RESULTS: Our study showed that at passage 10, mdx-MSCs exhibited increased heterochromatin, larger vacuoles, and more lysosomes under electron microscopy compared to C57BL/10-MSCs. C57BL/10-MSCs formed a few myotubes, while mdx-MSCs did not at the same passages. By passage 21, mdx-MSCs but not C57BL/10-MSCs had gradually lost their proliferative ability. In addition, a significant difference in the expression of CD34, not Sca-1 and CD11b, was observed between the MSCs from the 2 mice. CONCLUSION: Our current study reveals that the MSCs from the 2 mice, namely, C57BL/10 and mdx, exhibit differences in proliferative and myogenic abilities. The results suggest that the changes in mouse MSC behavior may be influenced by lack of dystrophin protein in mdx mouse. SN - 1471-2121 UR - https://www.unboundmedicine.com/medline/citation/18489762/Comparative_study_of_mesenchymal_stem_cells_from_C57BL/10_and_mdx_mice_ L2 - https://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-9-24 DB - PRIME DP - Unbound Medicine ER -