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Osteoblast interaction with DLC-coated Si substrates.
Acta Biomater. 2008 Sep; 4(5):1369-81.AB

Abstract

Diamond-like carbon (DLC) coating is a convenient means of modifying material surfaces that are sensitive to wear, such as titanium and silica substrates. This work aims to evaluate the osteoblast-like cells' response to DLC-coated Si (Si-DLC), which was treated under different conditions. DLC and deuterated DLC films were deposited by plasma-enhanced chemical vapor deposition to obtain a 200-nm-thick layer on all the samples. Three types of precursor gas were applied for deposition: pure methane (CH(4)), pure deuterated methane (CD(4)) and their half/half mixture. All surface treatments were performed under two different self-bias voltages (V(sb)): -400 and -600V. The modified surfaces were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, Rutherford backscattering spectroscopy, elastic recoil detection analysis, X-ray reflectometry and the sessile-drop method. MC3T3-E1 osteoblasts were cultured on the Si-DLC wafers for 3 and 6 days. Biological tests to measure cell proliferation, cell vitality, cell morphology and cell adhesion were performed. All DLC coatings produced a slightly more hydrophobic state than non-treated Si. Certain types of amorphous DLC coating, such as the surface treated under the V(sb) of -600V in pure methane (600CH(4)) or in pure deuterated methane (600CD(4)), offered a significantly higher cell proliferation rate to Si substrate. Scanning electron microscopy observations confirmed that the optimal cell adhesion behavior, among all the treated surfaces, occurred on the surface of the 600CH(4) and 600CD(4) groups, which showed increased amounts of filopodia and microvilli to enhance cell-environment exchange. In conclusion, DLC coating on Si could produce better surface stability and improved cellular responses.

Authors+Show Affiliations

Groupe de Recherche sur les Biomatériaux (GRB), Laboratoire de Biophysique, UPRES EA 1049, Faculté de Médecine, Université de Lille-2, 1 place de Verdun, 59045 Lille cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18495562

Citation

Chai, Feng, et al. "Osteoblast Interaction With DLC-coated Si Substrates." Acta Biomaterialia, vol. 4, no. 5, 2008, pp. 1369-81.
Chai F, Mathis N, Blanchemain N, et al. Osteoblast interaction with DLC-coated Si substrates. Acta Biomater. 2008;4(5):1369-81.
Chai, F., Mathis, N., Blanchemain, N., Meunier, C., & Hildebrand, H. F. (2008). Osteoblast interaction with DLC-coated Si substrates. Acta Biomaterialia, 4(5), 1369-81. https://doi.org/10.1016/j.actbio.2008.04.011
Chai F, et al. Osteoblast Interaction With DLC-coated Si Substrates. Acta Biomater. 2008;4(5):1369-81. PubMed PMID: 18495562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Osteoblast interaction with DLC-coated Si substrates. AU - Chai,Feng, AU - Mathis,Nicolas, AU - Blanchemain,Nicolas, AU - Meunier,Cathy, AU - Hildebrand,Hartmut F, Y1 - 2008/04/29/ PY - 2007/10/12/received PY - 2008/03/23/revised PY - 2008/04/04/accepted PY - 2008/5/23/pubmed PY - 2008/11/19/medline PY - 2008/5/23/entrez SP - 1369 EP - 81 JF - Acta biomaterialia JO - Acta Biomater VL - 4 IS - 5 N2 - Diamond-like carbon (DLC) coating is a convenient means of modifying material surfaces that are sensitive to wear, such as titanium and silica substrates. This work aims to evaluate the osteoblast-like cells' response to DLC-coated Si (Si-DLC), which was treated under different conditions. DLC and deuterated DLC films were deposited by plasma-enhanced chemical vapor deposition to obtain a 200-nm-thick layer on all the samples. Three types of precursor gas were applied for deposition: pure methane (CH(4)), pure deuterated methane (CD(4)) and their half/half mixture. All surface treatments were performed under two different self-bias voltages (V(sb)): -400 and -600V. The modified surfaces were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, Rutherford backscattering spectroscopy, elastic recoil detection analysis, X-ray reflectometry and the sessile-drop method. MC3T3-E1 osteoblasts were cultured on the Si-DLC wafers for 3 and 6 days. Biological tests to measure cell proliferation, cell vitality, cell morphology and cell adhesion were performed. All DLC coatings produced a slightly more hydrophobic state than non-treated Si. Certain types of amorphous DLC coating, such as the surface treated under the V(sb) of -600V in pure methane (600CH(4)) or in pure deuterated methane (600CD(4)), offered a significantly higher cell proliferation rate to Si substrate. Scanning electron microscopy observations confirmed that the optimal cell adhesion behavior, among all the treated surfaces, occurred on the surface of the 600CH(4) and 600CD(4) groups, which showed increased amounts of filopodia and microvilli to enhance cell-environment exchange. In conclusion, DLC coating on Si could produce better surface stability and improved cellular responses. SN - 1742-7061 UR - https://www.unboundmedicine.com/medline/citation/18495562/Osteoblast_interaction_with_DLC_coated_Si_substrates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1742-7061(08)00103-7 DB - PRIME DP - Unbound Medicine ER -