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Participation of 5-hydroxytryptamine in pain-related behavior induced by nucleus pulposus applied on the nerve root in rats.
Spine (Phila Pa 1976). 2008 May 20; 33(12):1330-6.S

Abstract

STUDY DESIGN

The role of 5-hydroxytryptamine (5-HT) in sciatica in lumbar disc herniation (LDH) in rats was investigated.

OBJECTIVE

We evaluated the effects of exogenous 5-HT applied on the nerve root on pain-related behavior, the release of endogenous 5-HT in plasma, and the expression of 5-HT2A receptors in dorsal root ganglion (DRG) in a rat LDH model.

SUMMARY OF BACKGROUND DATA

In previous studies, 5-HT2A receptor antagonists improved sciatica in patients with LDH and attenuated pain-related behavior induced by nucleus pulposus applied to the nerve root in rats.

METHODS

Adult female Sprague-Dawley rats were divided into four experimental groups [control group; low-dose (10 microg) 5-HT-group; high-dose (30 microg) 5-HT-group; and autologous nucleus pulposus (NP) and saline group] and each drug was applied to the L5 nerve root. Von Frey tests were used for pain-behavior testing. To assess levels of endogenous 5-HT released in capillaries surrounding inflamed nerve roots, we measured 5-hydroxyindole acetic acid (5-HIAA), a metabolite of 5-HT. Expression of 5-HT2A receptors in the left L5 DRG was examined by immunohistochemical and immunoblotting analyses in the control and NP groups.

RESULTS

Mechanical withdrawal thresholds of the high-dose 5-HT and the NP groups were significantly decreased after surgery compared with the control group and recovered after 14 days in the high-dose 5-HT group. 5-HIAA in plasma was increased by nucleus pulposus applied on the nerve root for 7 days after surgery. The expression of 5-HT2A receptors was enhanced in a time-dependent manner by nucleus pulposus.

CONCLUSION

Exogenous 5-HT to the nerve root induced pain-related behavior with short-lasting effects compared with the nucleus pulposus application. 5-HIAA content in plasma and expression of 5-HT2A receptors in DRG neurons increased early time points after the nucleus pulposus application. These results suggest that 5-HT plays a role in the early phase of the chemical pathogenesis of sciatica in LDH in rats.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, Fukushima City, Japan. kinshi@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18496345

Citation

Kato, Kinshi, et al. "Participation of 5-hydroxytryptamine in Pain-related Behavior Induced By Nucleus Pulposus Applied On the Nerve Root in Rats." Spine, vol. 33, no. 12, 2008, pp. 1330-6.
Kato K, Kikuchi S, Konno S, et al. Participation of 5-hydroxytryptamine in pain-related behavior induced by nucleus pulposus applied on the nerve root in rats. Spine (Phila Pa 1976). 2008;33(12):1330-6.
Kato, K., Kikuchi, S., Konno, S., & Sekiguchi, M. (2008). Participation of 5-hydroxytryptamine in pain-related behavior induced by nucleus pulposus applied on the nerve root in rats. Spine, 33(12), 1330-6. https://doi.org/10.1097/BRS.0b013e318173298b
Kato K, et al. Participation of 5-hydroxytryptamine in Pain-related Behavior Induced By Nucleus Pulposus Applied On the Nerve Root in Rats. Spine (Phila Pa 1976). 2008 May 20;33(12):1330-6. PubMed PMID: 18496345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Participation of 5-hydroxytryptamine in pain-related behavior induced by nucleus pulposus applied on the nerve root in rats. AU - Kato,Kinshi, AU - Kikuchi,Shin-ichi, AU - Konno,Shin-ichi, AU - Sekiguchi,Miho, PY - 2008/5/23/pubmed PY - 2008/7/4/medline PY - 2008/5/23/entrez SP - 1330 EP - 6 JF - Spine JO - Spine (Phila Pa 1976) VL - 33 IS - 12 N2 - STUDY DESIGN: The role of 5-hydroxytryptamine (5-HT) in sciatica in lumbar disc herniation (LDH) in rats was investigated. OBJECTIVE: We evaluated the effects of exogenous 5-HT applied on the nerve root on pain-related behavior, the release of endogenous 5-HT in plasma, and the expression of 5-HT2A receptors in dorsal root ganglion (DRG) in a rat LDH model. SUMMARY OF BACKGROUND DATA: In previous studies, 5-HT2A receptor antagonists improved sciatica in patients with LDH and attenuated pain-related behavior induced by nucleus pulposus applied to the nerve root in rats. METHODS: Adult female Sprague-Dawley rats were divided into four experimental groups [control group; low-dose (10 microg) 5-HT-group; high-dose (30 microg) 5-HT-group; and autologous nucleus pulposus (NP) and saline group] and each drug was applied to the L5 nerve root. Von Frey tests were used for pain-behavior testing. To assess levels of endogenous 5-HT released in capillaries surrounding inflamed nerve roots, we measured 5-hydroxyindole acetic acid (5-HIAA), a metabolite of 5-HT. Expression of 5-HT2A receptors in the left L5 DRG was examined by immunohistochemical and immunoblotting analyses in the control and NP groups. RESULTS: Mechanical withdrawal thresholds of the high-dose 5-HT and the NP groups were significantly decreased after surgery compared with the control group and recovered after 14 days in the high-dose 5-HT group. 5-HIAA in plasma was increased by nucleus pulposus applied on the nerve root for 7 days after surgery. The expression of 5-HT2A receptors was enhanced in a time-dependent manner by nucleus pulposus. CONCLUSION: Exogenous 5-HT to the nerve root induced pain-related behavior with short-lasting effects compared with the nucleus pulposus application. 5-HIAA content in plasma and expression of 5-HT2A receptors in DRG neurons increased early time points after the nucleus pulposus application. These results suggest that 5-HT plays a role in the early phase of the chemical pathogenesis of sciatica in LDH in rats. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/18496345/Participation_of_5_hydroxytryptamine_in_pain_related_behavior_induced_by_nucleus_pulposus_applied_on_the_nerve_root_in_rats_ L2 - https://doi.org/10.1097/BRS.0b013e318173298b DB - PRIME DP - Unbound Medicine ER -