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Contralateral neuropathic pain and neuropathology in dorsal root ganglion and spinal cord following hemilateral nerve injury in rats.
Spine (Phila Pa 1976). 2008 May 20; 33(12):1344-51.S

Abstract

STUDY DESIGN

The contralateral pain-related behavioral and immunohistochemical changes after hemilateral spinal nerve injury in rats were investigated.

OBJECTIVES

We evaluated the longitudinal changes in contralateral mechanical allodynia, expression of tumor necrosis factor (TNF)-alpha and glial fibrillary acidic protein (GFAP)-positive satellite cells in the contralateral dorsal root ganglion (DRG), and expression of astrocytes and microglia in the contralateral spinal dorsal horn after hemilateral spinal nerve injury in rats.

SUMMARY OF BACKGROUND DATA

In previous studies, hemilateral nerve injury has sometimes induced contralateral neuropathic pain. TNF-alpha expression and glial cell reactions in the DRG and spinal cord play an important role in the neuropathic pain state, and TNF-alpha is released from glial cells in the nervous system.

METHODS

Adult male Sprague-Dawley rats were used. The spinal L5 nerve distal to the DRG was crushed once for 3 seconds. At days 2, 7, 14, and 21 after surgery, mechanical allodynia was determined in bilateral hind paws by the von Frey test. Expression of TNF-alpha and GFAP in bilateral L5 DRGs and expression of GFAP and ionized calcium-binding adaptor molecule-1 (Iba-1) in bilateral L5 spinal dorsal horns were studied using immunohistochemistry and immunoblotting.

RESULTS

Mechanical withdrawal threshold of the ipsilateral hind paw was significantly decreased for 21 days. Conversely, mechanical withdrawal threshold of the contralateral hind paw was significantly decreased from 5 to 10 g for 7 days, and was <5 g at days 14 and 21. TNF-alpha expression and GFAP-positive satellite cells in the contralateral DRG significantly increased from day 7 to day 21. In the contralateral spinal dorsal horn, GFAP-positive astrocytes significantly increased for 21 days, but Iba-1 was not significant.

CONCLUSION

These results suggest that contralateral mechanical allodynia induced by hemilateral spinal nerve injury is associated with upregulation of satellite cells and TNF-alpha in the contralateral DRG. In addition, our results suggest that spinal astrocytes play an important role in these contralateral changes.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, Fukushima, Japan. hatashi@fmu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18496347

Citation

Hatashita, Satoshi, et al. "Contralateral Neuropathic Pain and Neuropathology in Dorsal Root Ganglion and Spinal Cord Following Hemilateral Nerve Injury in Rats." Spine, vol. 33, no. 12, 2008, pp. 1344-51.
Hatashita S, Sekiguchi M, Kobayashi H, et al. Contralateral neuropathic pain and neuropathology in dorsal root ganglion and spinal cord following hemilateral nerve injury in rats. Spine (Phila Pa 1976). 2008;33(12):1344-51.
Hatashita, S., Sekiguchi, M., Kobayashi, H., Konno, S., & Kikuchi, S. (2008). Contralateral neuropathic pain and neuropathology in dorsal root ganglion and spinal cord following hemilateral nerve injury in rats. Spine, 33(12), 1344-51. https://doi.org/10.1097/BRS.0b013e3181733188
Hatashita S, et al. Contralateral Neuropathic Pain and Neuropathology in Dorsal Root Ganglion and Spinal Cord Following Hemilateral Nerve Injury in Rats. Spine (Phila Pa 1976). 2008 May 20;33(12):1344-51. PubMed PMID: 18496347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contralateral neuropathic pain and neuropathology in dorsal root ganglion and spinal cord following hemilateral nerve injury in rats. AU - Hatashita,Satoshi, AU - Sekiguchi,Miho, AU - Kobayashi,Hideo, AU - Konno,Shin-ichi, AU - Kikuchi,Shin-ichi, PY - 2008/5/23/pubmed PY - 2008/7/4/medline PY - 2008/5/23/entrez SP - 1344 EP - 51 JF - Spine JO - Spine (Phila Pa 1976) VL - 33 IS - 12 N2 - STUDY DESIGN: The contralateral pain-related behavioral and immunohistochemical changes after hemilateral spinal nerve injury in rats were investigated. OBJECTIVES: We evaluated the longitudinal changes in contralateral mechanical allodynia, expression of tumor necrosis factor (TNF)-alpha and glial fibrillary acidic protein (GFAP)-positive satellite cells in the contralateral dorsal root ganglion (DRG), and expression of astrocytes and microglia in the contralateral spinal dorsal horn after hemilateral spinal nerve injury in rats. SUMMARY OF BACKGROUND DATA: In previous studies, hemilateral nerve injury has sometimes induced contralateral neuropathic pain. TNF-alpha expression and glial cell reactions in the DRG and spinal cord play an important role in the neuropathic pain state, and TNF-alpha is released from glial cells in the nervous system. METHODS: Adult male Sprague-Dawley rats were used. The spinal L5 nerve distal to the DRG was crushed once for 3 seconds. At days 2, 7, 14, and 21 after surgery, mechanical allodynia was determined in bilateral hind paws by the von Frey test. Expression of TNF-alpha and GFAP in bilateral L5 DRGs and expression of GFAP and ionized calcium-binding adaptor molecule-1 (Iba-1) in bilateral L5 spinal dorsal horns were studied using immunohistochemistry and immunoblotting. RESULTS: Mechanical withdrawal threshold of the ipsilateral hind paw was significantly decreased for 21 days. Conversely, mechanical withdrawal threshold of the contralateral hind paw was significantly decreased from 5 to 10 g for 7 days, and was <5 g at days 14 and 21. TNF-alpha expression and GFAP-positive satellite cells in the contralateral DRG significantly increased from day 7 to day 21. In the contralateral spinal dorsal horn, GFAP-positive astrocytes significantly increased for 21 days, but Iba-1 was not significant. CONCLUSION: These results suggest that contralateral mechanical allodynia induced by hemilateral spinal nerve injury is associated with upregulation of satellite cells and TNF-alpha in the contralateral DRG. In addition, our results suggest that spinal astrocytes play an important role in these contralateral changes. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/18496347/Contralateral_neuropathic_pain_and_neuropathology_in_dorsal_root_ganglion_and_spinal_cord_following_hemilateral_nerve_injury_in_rats_ L2 - https://doi.org/10.1097/BRS.0b013e3181733188 DB - PRIME DP - Unbound Medicine ER -