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Endocannabinoid- and mGluR5-dependent short-term synaptic depression in an isolated neuron/bouton preparation from the hippocampal CA1 region.
J Neurophysiol. 2008 Aug; 100(2):1041-52.JN

Abstract

Endocannabinoids released from the postsynaptic neuronal membrane can activate presynaptic CB1 receptors and inhibit neurotransmitter release. In hippocampal slices, depolarization of the CA1 pyramidal neurons elicits an endocannabinoid-mediated inhibition of gamma-aminobutyric acid release known as depolarization-induced suppression of inhibition (DSI). Using the highly reduced neuron/synaptic bouton preparation from the CA1 region of hippocampus, we have begun to examine endocannabinoid-dependent short-term depression (STD) of inhibitory synaptic transmission under well-controlled physiological and pharmacological conditions in an environment free of other cells. Application of the CB1 synthetic agonist WIN55212-2 and endogenous cannabinoids 2-AG and anandamide produced a decrease in spontaneous inhibitory postsynaptic current (sIPSC) frequency and amplitude, indicating the presence of CB1 receptors at synapses in this preparation. Endocannabinoid-dependent STD is different from DSI found in hippocampal slices and the neuron/bouton preparation from basolateral amygdala (BLA) since depolarization alone was not sufficient to induce suppression of sIPSCs. However, concurrent application of the metabotropic glutamate receptor (mGluR) agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) and postsynaptic depolarization resulted in a transient (30-50 s) decrease in sIPSC frequency and amplitude. Application of DHPG alone had no effect on sIPSCs. The depolarization/DHPG-induced STD was blocked by the CB1 antagonist SR141716A and the mGluR5 antagonist MPEP and was sensitive to intracellular calcium concentration. Comparing the present findings with earlier work in hippocampal slices and BLA, it appears that endocannabinoid release is less robust in isolated hippocampal neurons.

Authors+Show Affiliations

Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, National Institute of Health, Bethesda, MD 20892-8115, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

18497350

Citation

Sheinin, Anton, et al. "Endocannabinoid- and mGluR5-dependent Short-term Synaptic Depression in an Isolated Neuron/bouton Preparation From the Hippocampal CA1 Region." Journal of Neurophysiology, vol. 100, no. 2, 2008, pp. 1041-52.
Sheinin A, Talani G, Davis MI, et al. Endocannabinoid- and mGluR5-dependent short-term synaptic depression in an isolated neuron/bouton preparation from the hippocampal CA1 region. J Neurophysiol. 2008;100(2):1041-52.
Sheinin, A., Talani, G., Davis, M. I., & Lovinger, D. M. (2008). Endocannabinoid- and mGluR5-dependent short-term synaptic depression in an isolated neuron/bouton preparation from the hippocampal CA1 region. Journal of Neurophysiology, 100(2), 1041-52. https://doi.org/10.1152/jn.90226.2008
Sheinin A, et al. Endocannabinoid- and mGluR5-dependent Short-term Synaptic Depression in an Isolated Neuron/bouton Preparation From the Hippocampal CA1 Region. J Neurophysiol. 2008;100(2):1041-52. PubMed PMID: 18497350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endocannabinoid- and mGluR5-dependent short-term synaptic depression in an isolated neuron/bouton preparation from the hippocampal CA1 region. AU - Sheinin,Anton, AU - Talani,Giuseppe, AU - Davis,Margaret I, AU - Lovinger,David M, Y1 - 2008/05/21/ PY - 2008/5/24/pubmed PY - 2008/11/6/medline PY - 2008/5/24/entrez SP - 1041 EP - 52 JF - Journal of neurophysiology JO - J Neurophysiol VL - 100 IS - 2 N2 - Endocannabinoids released from the postsynaptic neuronal membrane can activate presynaptic CB1 receptors and inhibit neurotransmitter release. In hippocampal slices, depolarization of the CA1 pyramidal neurons elicits an endocannabinoid-mediated inhibition of gamma-aminobutyric acid release known as depolarization-induced suppression of inhibition (DSI). Using the highly reduced neuron/synaptic bouton preparation from the CA1 region of hippocampus, we have begun to examine endocannabinoid-dependent short-term depression (STD) of inhibitory synaptic transmission under well-controlled physiological and pharmacological conditions in an environment free of other cells. Application of the CB1 synthetic agonist WIN55212-2 and endogenous cannabinoids 2-AG and anandamide produced a decrease in spontaneous inhibitory postsynaptic current (sIPSC) frequency and amplitude, indicating the presence of CB1 receptors at synapses in this preparation. Endocannabinoid-dependent STD is different from DSI found in hippocampal slices and the neuron/bouton preparation from basolateral amygdala (BLA) since depolarization alone was not sufficient to induce suppression of sIPSCs. However, concurrent application of the metabotropic glutamate receptor (mGluR) agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) and postsynaptic depolarization resulted in a transient (30-50 s) decrease in sIPSC frequency and amplitude. Application of DHPG alone had no effect on sIPSCs. The depolarization/DHPG-induced STD was blocked by the CB1 antagonist SR141716A and the mGluR5 antagonist MPEP and was sensitive to intracellular calcium concentration. Comparing the present findings with earlier work in hippocampal slices and BLA, it appears that endocannabinoid release is less robust in isolated hippocampal neurons. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/18497350/Endocannabinoid__and_mGluR5_dependent_short_term_synaptic_depression_in_an_isolated_neuron/bouton_preparation_from_the_hippocampal_CA1_region_ L2 - https://journals.physiology.org/doi/10.1152/jn.90226.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -