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Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing.
Biochem J. 2008 Oct 01; 415(1):123-34.BJ

Abstract

TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete deficiency of TAL. In the present study, microarray and follow-up Western-blot, enzyme-activity and metabolic studies of TALDeltaS171 TD (TAL-deficient) lymphoblasts revealed co-ordinated changes in the expression of genes involved in the PPP, mitochondrial biogenesis, oxidative stress, and Ca(2+) fluxing. Sedoheptulose 7-phosphate was accumulated, whereas G6P (glucose 6-phosphate) was depleted, indicating a failure to recycle G6P for the oxidative branch of the PPP. Nucleotide analysis showed depletion of NADPH and NAD(+) and accumulation of ADP-ribose. TD cells have diminished Deltapsi(m) (mitochondrial transmembrane potential) and increased mitochondrial mass associated with increased production of nitric oxide and ATP. TAL deficiency resulted in enhanced spontaneous and H(2)O(2)-induced apoptosis. TD lymphoblasts showed increased expression of CD38, which hydrolyses NAD(+) into ADP-ribose, a trigger of Ca(2+) release from the endoplasmic reticulum that, in turn, facilitated CD20-induced apoptosis. By contrast, TD cells were resistant to CD95/Fas-induced apoptosis, owing to a dependence of caspase activity on redox-sensitive cysteine residues. Normalization of TAL activity by adeno-associated-virus-mediated gene transfer reversed the elevated CD38 expression, ATP and Ca(2+) levels, suppressed H(2)O(2)- and CD20-induced apoptosis and enhanced Fas-induced cell death. The present study identified the TAL deficiency as a modulator of mitochondrial homoeostasis, Ca(2+) fluxing and apoptosis.

Authors+Show Affiliations

Department of Medicine, SUNY Upstate Medical University, Syracuse, NY 13210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18498245

Citation

Qian, Yueming, et al. "Transaldolase Deficiency Influences the Pentose Phosphate Pathway, Mitochondrial Homoeostasis and Apoptosis Signal Processing." The Biochemical Journal, vol. 415, no. 1, 2008, pp. 123-34.
Qian Y, Banerjee S, Grossman CE, et al. Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing. Biochem J. 2008;415(1):123-34.
Qian, Y., Banerjee, S., Grossman, C. E., Amidon, W., Nagy, G., Barcza, M., Niland, B., Karp, D. R., Middleton, F. A., Banki, K., & Perl, A. (2008). Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing. The Biochemical Journal, 415(1), 123-34. https://doi.org/10.1042/BJ20080722
Qian Y, et al. Transaldolase Deficiency Influences the Pentose Phosphate Pathway, Mitochondrial Homoeostasis and Apoptosis Signal Processing. Biochem J. 2008 Oct 1;415(1):123-34. PubMed PMID: 18498245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing. AU - Qian,Yueming, AU - Banerjee,Sanjay, AU - Grossman,Craig E, AU - Amidon,Wendy, AU - Nagy,Gyorgy, AU - Barcza,Maureen, AU - Niland,Brian, AU - Karp,David R, AU - Middleton,Frank A, AU - Banki,Katalin, AU - Perl,Andras, PY - 2008/5/24/pubmed PY - 2008/10/7/medline PY - 2008/5/24/entrez SP - 123 EP - 34 JF - The Biochemical journal JO - Biochem. J. VL - 415 IS - 1 N2 - TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete deficiency of TAL. In the present study, microarray and follow-up Western-blot, enzyme-activity and metabolic studies of TALDeltaS171 TD (TAL-deficient) lymphoblasts revealed co-ordinated changes in the expression of genes involved in the PPP, mitochondrial biogenesis, oxidative stress, and Ca(2+) fluxing. Sedoheptulose 7-phosphate was accumulated, whereas G6P (glucose 6-phosphate) was depleted, indicating a failure to recycle G6P for the oxidative branch of the PPP. Nucleotide analysis showed depletion of NADPH and NAD(+) and accumulation of ADP-ribose. TD cells have diminished Deltapsi(m) (mitochondrial transmembrane potential) and increased mitochondrial mass associated with increased production of nitric oxide and ATP. TAL deficiency resulted in enhanced spontaneous and H(2)O(2)-induced apoptosis. TD lymphoblasts showed increased expression of CD38, which hydrolyses NAD(+) into ADP-ribose, a trigger of Ca(2+) release from the endoplasmic reticulum that, in turn, facilitated CD20-induced apoptosis. By contrast, TD cells were resistant to CD95/Fas-induced apoptosis, owing to a dependence of caspase activity on redox-sensitive cysteine residues. Normalization of TAL activity by adeno-associated-virus-mediated gene transfer reversed the elevated CD38 expression, ATP and Ca(2+) levels, suppressed H(2)O(2)- and CD20-induced apoptosis and enhanced Fas-induced cell death. The present study identified the TAL deficiency as a modulator of mitochondrial homoeostasis, Ca(2+) fluxing and apoptosis. SN - 1470-8728 UR - https://www.unboundmedicine.com/medline/citation/18498245/Transaldolase_deficiency_influences_the_pentose_phosphate_pathway_mitochondrial_homoeostasis_and_apoptosis_signal_processing_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BJ20080722 DB - PRIME DP - Unbound Medicine ER -