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Anti-elastase and anti-hyaluronidase of phenolic substance from Areca catechu as a new anti-ageing agent.
Int J Cosmet Sci. 2001 Dec; 23(6):341-6.IJ

Abstract

We have previously screened 150 medicinal plants for the inhibition of elastase and found significant inhibitory effects of the extracts of Areca catechu L. on the ageing and inflammation of skin tissues. To isolate and identify the compounds having biological activity, they were further purified by each fraction of solvents, silica gel column chromatography, preparative TLC and reversed-phase HPLC. The peak in HPLC, which coincided with the inhibitory activity against elastase, was identified as a phenolic substance by using various colorimetric methods, UV and IR. IC(50) values of this phenolic substance were 26.9 mug mL(-1) for porcine pancreatic elastase (PPE) and 60.8 mug mL(-1) for human neutrophil elastase (HNE). This phenolic substance showed more potent activity than that of reference compounds, oleanolic acid (76.5 mug mL(-1) for PPE, 219.2 mug mL(-1) for HNE) and ursolic acid (31.0 mug mL(-1) for PPE, 118.6 mug mL(-1) for HNE). According to the Lineweaver-Burk plots, the inhibition against both PPE and HNE by this phenolic substance was competitive inhibition with the substrate. The phenolic substance from A. catechu effectively inhibited hyaluronidase activity (IC(50) : 210 mug mL(-1)). These results suggest that the phenolic substance purified from A. catechu has an anti-ageing effect by protecting connective tissue proteins.

Authors+Show Affiliations

R & D Center, Coreana Cosmetic Co. Ltd, Jeongchon-ri, Seonggeo-Eup, Cheonan-Si, Chungnam, 330-830, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18498484

Citation

Lee, K K., et al. "Anti-elastase and Anti-hyaluronidase of Phenolic Substance From Areca Catechu as a New Anti-ageing Agent." International Journal of Cosmetic Science, vol. 23, no. 6, 2001, pp. 341-6.
Lee KK, Cho JJ, Park EJ, et al. Anti-elastase and anti-hyaluronidase of phenolic substance from Areca catechu as a new anti-ageing agent. Int J Cosmet Sci. 2001;23(6):341-6.
Lee, K. K., Cho, J. J., Park, E. J., & Choi, J. D. (2001). Anti-elastase and anti-hyaluronidase of phenolic substance from Areca catechu as a new anti-ageing agent. International Journal of Cosmetic Science, 23(6), 341-6. https://doi.org/10.1046/j.0412-5463.2001.00102.x
Lee KK, et al. Anti-elastase and Anti-hyaluronidase of Phenolic Substance From Areca Catechu as a New Anti-ageing Agent. Int J Cosmet Sci. 2001;23(6):341-6. PubMed PMID: 18498484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-elastase and anti-hyaluronidase of phenolic substance from Areca catechu as a new anti-ageing agent. AU - Lee,K K, AU - Cho,J J, AU - Park,E J, AU - Choi,J D, PY - 2008/5/24/pubmed PY - 2008/5/24/medline PY - 2008/5/24/entrez SP - 341 EP - 6 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 23 IS - 6 N2 - We have previously screened 150 medicinal plants for the inhibition of elastase and found significant inhibitory effects of the extracts of Areca catechu L. on the ageing and inflammation of skin tissues. To isolate and identify the compounds having biological activity, they were further purified by each fraction of solvents, silica gel column chromatography, preparative TLC and reversed-phase HPLC. The peak in HPLC, which coincided with the inhibitory activity against elastase, was identified as a phenolic substance by using various colorimetric methods, UV and IR. IC(50) values of this phenolic substance were 26.9 mug mL(-1) for porcine pancreatic elastase (PPE) and 60.8 mug mL(-1) for human neutrophil elastase (HNE). This phenolic substance showed more potent activity than that of reference compounds, oleanolic acid (76.5 mug mL(-1) for PPE, 219.2 mug mL(-1) for HNE) and ursolic acid (31.0 mug mL(-1) for PPE, 118.6 mug mL(-1) for HNE). According to the Lineweaver-Burk plots, the inhibition against both PPE and HNE by this phenolic substance was competitive inhibition with the substrate. The phenolic substance from A. catechu effectively inhibited hyaluronidase activity (IC(50) : 210 mug mL(-1)). These results suggest that the phenolic substance purified from A. catechu has an anti-ageing effect by protecting connective tissue proteins. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/18498484/Anti_elastase_and_anti_hyaluronidase_of_phenolic_substance_from_Areca_catechu_as_a_new_anti_ageing_agent_ L2 - https://doi.org/10.1046/j.0412-5463.2001.00102.x DB - PRIME DP - Unbound Medicine ER -
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