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Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro.
FEBS Lett. 2008 Jun 11; 582(13):1901-6.FL

Abstract

MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that 59Fe uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1.

Links

Publisher Full Text
linkinghub.elsevier.com
doi.org

Authors+Show Affiliations

Wyman S
King's College London, Iron Metabolism Group, Nutritional Sciences Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom.
Simpson RJ
No affiliation info available
McKie AT
No affiliation info available
Sharp PA
No affiliation info available

MeSH

AnimalsCation Transport ProteinsCell LineCopperCopper Transporter 1Cytochrome b GroupDogsFMN ReductaseGreen Fluorescent ProteinsIon TransportIronMiceOxidoreductasesRecombinant Fusion ProteinsSubstrate SpecificityTransfection

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18498772

Citation

Wyman, Steven, et al. "Dcytb (Cybrd1) Functions as Both a Ferric and a Cupric Reductase in Vitro." FEBS Letters, vol. 582, no. 13, 2008, pp. 1901-6.
Wyman S, Simpson RJ, McKie AT, et al. Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro. FEBS Lett. 2008;582(13):1901-6.
Wyman, S., Simpson, R. J., McKie, A. T., & Sharp, P. A. (2008). Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro. FEBS Letters, 582(13), 1901-6. https://doi.org/10.1016/j.febslet.2008.05.010
Wyman S, et al. Dcytb (Cybrd1) Functions as Both a Ferric and a Cupric Reductase in Vitro. FEBS Lett. 2008 Jun 11;582(13):1901-6. PubMed PMID: 18498772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro. AU - Wyman,Steven, AU - Simpson,Robert J, AU - McKie,Andrew T, AU - Sharp,Paul A, Y1 - 2008/05/20/ PY - 2008/04/02/received PY - 2008/04/18/revised PY - 2008/05/07/accepted PY - 2008/5/24/pubmed PY - 2008/7/26/medline PY - 2008/5/24/entrez SP - 1901 EP - 6 JF - FEBS letters JO - FEBS Lett VL - 582 IS - 13 N2 - MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that 59Fe uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/18498772/Dcytb__Cybrd1__functions_as_both_a_ferric_and_a_cupric_reductase_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(08)00410-9 DB - PRIME DP - Unbound Medicine ER -