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Extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by Kluyveromyces marxianus NRRL Y-7571.
Appl Biochem Biotechnol. 2008 Jun; 149(3):195-203.AB

Abstract

Production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. This work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and Kluyveromyces marxianus NRRL Y-7571. Kinetics of extraction was followed varying the kind of solvent used. After determining the best solvent, an experimental design was carried out to study the effect of the solid/liquid ratio (1:10-1:20), extraction temperature (20-53 degrees C), and stirring rate (50-177 rpm). Results showed that maximum yield was obtained when sodium acetate buffer 0.1 M pH 4.8 was used, using a solid/liquid ratio of 1:10, at 53 degrees C and 150 rpm for 40 min.

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Authors+Show Affiliations

Bender JP
Departamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, Av. Sete de Setembro 1621, Erechim 99700-000, Brazil.
Mazutti MA
No affiliation info available
Di Luccio M
No affiliation info available
Treichel H
No affiliation info available

MeSH

FermentationGlycoside HydrolasesKineticsKluyveromycesOsmolar ConcentrationSaccharum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18500581

Citation

Bender, João Paulo, et al. "Extraction of Inulinase Obtained By Solid State Fermentation of Sugarcane Bagasse By Kluyveromyces Marxianus NRRL Y-7571." Applied Biochemistry and Biotechnology, vol. 149, no. 3, 2008, pp. 195-203.
Bender JP, Mazutti MA, Di Luccio M, et al. Extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by Kluyveromyces marxianus NRRL Y-7571. Appl Biochem Biotechnol. 2008;149(3):195-203.
Bender, J. P., Mazutti, M. A., Di Luccio, M., & Treichel, H. (2008). Extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by Kluyveromyces marxianus NRRL Y-7571. Applied Biochemistry and Biotechnology, 149(3), 195-203. https://doi.org/10.1007/s12010-007-8111-1
Bender JP, et al. Extraction of Inulinase Obtained By Solid State Fermentation of Sugarcane Bagasse By Kluyveromyces Marxianus NRRL Y-7571. Appl Biochem Biotechnol. 2008;149(3):195-203. PubMed PMID: 18500581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by Kluyveromyces marxianus NRRL Y-7571. AU - Bender,João Paulo, AU - Mazutti,Marcio Antônio, AU - Di Luccio,Marco, AU - Treichel,Helen, Y1 - 2008/01/19/ PY - 2007/08/15/received PY - 2007/11/21/accepted PY - 2008/5/27/pubmed PY - 2008/9/4/medline PY - 2008/5/27/entrez SP - 195 EP - 203 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 149 IS - 3 N2 - Production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. This work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and Kluyveromyces marxianus NRRL Y-7571. Kinetics of extraction was followed varying the kind of solvent used. After determining the best solvent, an experimental design was carried out to study the effect of the solid/liquid ratio (1:10-1:20), extraction temperature (20-53 degrees C), and stirring rate (50-177 rpm). Results showed that maximum yield was obtained when sodium acetate buffer 0.1 M pH 4.8 was used, using a solid/liquid ratio of 1:10, at 53 degrees C and 150 rpm for 40 min. SN - 0273-2289 UR - https://www.unboundmedicine.com/medline/citation/18500581/Extraction_of_inulinase_obtained_by_solid_state_fermentation_of_sugarcane_bagasse_by_Kluyveromyces_marxianus_NRRL_Y_7571_ L2 - https://dx.doi.org/10.1007/s12010-007-8111-1 DB - PRIME DP - Unbound Medicine ER -