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Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification.
J Colloid Interface Sci. 2008 Jul 15; 323(2):267-73.JC

Abstract

Relatively uniform-sized poly(lactide-co-ethylene glycol) (PELA) microspheres with high encapsulation efficiency were prepared rapidly by a novel method combining emulsion-solvent extraction and premix membrane emulsification. Briefly, preparation of coarse double emulsions was followed by additional premix membrane emulsification, and antigen-loaded microspheres were obtained by further solidification. Under the optimum condition, the particle size was about 1 mum and the coefficient of variation (CV) value was 18.9%. Confocal laser scanning microscope and flow cytometer analysis showed that the inner droplets were small and evenly dispersed and the antigen was loaded uniformly in each microsphere when sonication technique was occupied to prepare primary emulsion. Distribution pattern of PEG segment played important role on the properties of microspheres. Compared with triblock copolymer PLA-PEG-PLA, the diblock copolymer PLA-mPEG yielded a more stable interfacial layer at the interface of oil and water phase, and thus was more suitable to stabilize primary emulsion and protect coalescence of inner droplets and external water phase, resulting in high encapsulation efficiency (90.4%). On the other hand, solidification rate determined the time for coalescence during microspheres fabrication, and thus affected encapsulation efficiency. Taken together, improving the polymer properties and solidification rate are considered as two effective strategies to yield high encapsulation.

Authors+Show Affiliations

State Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18501376

Citation

Wei, Qiang, et al. "Preparation of Uniform-sized PELA Microspheres With High Encapsulation Efficiency of Antigen By Premix Membrane Emulsification." Journal of Colloid and Interface Science, vol. 323, no. 2, 2008, pp. 267-73.
Wei Q, Wei W, Tian R, et al. Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification. J Colloid Interface Sci. 2008;323(2):267-73.
Wei, Q., Wei, W., Tian, R., Wang, L. Y., Su, Z. G., & Ma, G. H. (2008). Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification. Journal of Colloid and Interface Science, 323(2), 267-73. https://doi.org/10.1016/j.jcis.2008.04.058
Wei Q, et al. Preparation of Uniform-sized PELA Microspheres With High Encapsulation Efficiency of Antigen By Premix Membrane Emulsification. J Colloid Interface Sci. 2008 Jul 15;323(2):267-73. PubMed PMID: 18501376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of uniform-sized PELA microspheres with high encapsulation efficiency of antigen by premix membrane emulsification. AU - Wei,Qiang, AU - Wei,Wei, AU - Tian,Rui, AU - Wang,Lian-Yan, AU - Su,Zhi-Guo, AU - Ma,Guang-Hui, Y1 - 2008/04/30/ PY - 2008/02/03/received PY - 2008/04/17/revised PY - 2008/04/19/accepted PY - 2008/5/27/pubmed PY - 2008/7/29/medline PY - 2008/5/27/entrez SP - 267 EP - 73 JF - Journal of colloid and interface science JO - J Colloid Interface Sci VL - 323 IS - 2 N2 - Relatively uniform-sized poly(lactide-co-ethylene glycol) (PELA) microspheres with high encapsulation efficiency were prepared rapidly by a novel method combining emulsion-solvent extraction and premix membrane emulsification. Briefly, preparation of coarse double emulsions was followed by additional premix membrane emulsification, and antigen-loaded microspheres were obtained by further solidification. Under the optimum condition, the particle size was about 1 mum and the coefficient of variation (CV) value was 18.9%. Confocal laser scanning microscope and flow cytometer analysis showed that the inner droplets were small and evenly dispersed and the antigen was loaded uniformly in each microsphere when sonication technique was occupied to prepare primary emulsion. Distribution pattern of PEG segment played important role on the properties of microspheres. Compared with triblock copolymer PLA-PEG-PLA, the diblock copolymer PLA-mPEG yielded a more stable interfacial layer at the interface of oil and water phase, and thus was more suitable to stabilize primary emulsion and protect coalescence of inner droplets and external water phase, resulting in high encapsulation efficiency (90.4%). On the other hand, solidification rate determined the time for coalescence during microspheres fabrication, and thus affected encapsulation efficiency. Taken together, improving the polymer properties and solidification rate are considered as two effective strategies to yield high encapsulation. SN - 0021-9797 UR - https://www.unboundmedicine.com/medline/citation/18501376/Preparation_of_uniform_sized_PELA_microspheres_with_high_encapsulation_efficiency_of_antigen_by_premix_membrane_emulsification_ DB - PRIME DP - Unbound Medicine ER -