Citation
Li, Chengjun, et al. "H5N1 Influenza Marker Vaccine for Serological Differentiation Between Vaccinated and Infected Chickens." Biochemical and Biophysical Research Communications, vol. 372, no. 2, 2008, pp. 293-7.
Li C, Ping J, Jing B, et al. H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. Biochem Biophys Res Commun. 2008;372(2):293-7.
Li, C., Ping, J., Jing, B., Deng, G., Jiang, Y., Li, Y., Tian, G., Yu, K., Bu, Z., & Chen, H. (2008). H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. Biochemical and Biophysical Research Communications, 372(2), 293-7. https://doi.org/10.1016/j.bbrc.2008.05.057
Li C, et al. H5N1 Influenza Marker Vaccine for Serological Differentiation Between Vaccinated and Infected Chickens. Biochem Biophys Res Commun. 2008 Jul 25;372(2):293-7. PubMed PMID: 18501701.
TY - JOUR
T1 - H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens.
AU - Li,Chengjun,
AU - Ping,Jihui,
AU - Jing,Bo,
AU - Deng,Guohua,
AU - Jiang,Yongping,
AU - Li,Yanbing,
AU - Tian,Guobin,
AU - Yu,Kangzhen,
AU - Bu,Zhigao,
AU - Chen,Hualan,
Y1 - 2008/05/22/
PY - 2008/05/09/received
PY - 2008/05/14/accepted
PY - 2008/5/27/pubmed
PY - 2008/8/13/medline
PY - 2008/5/27/entrez
SP - 293
EP - 7
JF - Biochemical and biophysical research communications
JO - Biochem Biophys Res Commun
VL - 372
IS - 2
N2 - Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens.
SN - 1090-2104
UR - https://www.unboundmedicine.com/medline/citation/18501701/H5N1_influenza_marker_vaccine_for_serological_differentiation_between_vaccinated_and_infected_chickens_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)00975-3
DB - PRIME
DP - Unbound Medicine
ER -