TY - JOUR T1 - H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. AU - Li,Chengjun, AU - Ping,Jihui, AU - Jing,Bo, AU - Deng,Guohua, AU - Jiang,Yongping, AU - Li,Yanbing, AU - Tian,Guobin, AU - Yu,Kangzhen, AU - Bu,Zhigao, AU - Chen,Hualan, Y1 - 2008/05/22/ PY - 2008/05/09/received PY - 2008/05/14/accepted PY - 2008/5/27/pubmed PY - 2008/8/13/medline PY - 2008/5/27/entrez SP - 293 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 372 IS - 2 N2 - Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18501701/H5N1_influenza_marker_vaccine_for_serological_differentiation_between_vaccinated_and_infected_chickens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)00975-3 DB - PRIME DP - Unbound Medicine ER -