Tags

Type your tag names separated by a space and hit enter

H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens.
Biochem Biophys Res Commun. 2008 Jul 25; 372(2):293-7.BB

Abstract

Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens.

Authors+Show Affiliations

Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, Heilongjiang 150001, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18501701

Citation

Li, Chengjun, et al. "H5N1 Influenza Marker Vaccine for Serological Differentiation Between Vaccinated and Infected Chickens." Biochemical and Biophysical Research Communications, vol. 372, no. 2, 2008, pp. 293-7.
Li C, Ping J, Jing B, et al. H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. Biochem Biophys Res Commun. 2008;372(2):293-7.
Li, C., Ping, J., Jing, B., Deng, G., Jiang, Y., Li, Y., Tian, G., Yu, K., Bu, Z., & Chen, H. (2008). H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. Biochemical and Biophysical Research Communications, 372(2), 293-7. https://doi.org/10.1016/j.bbrc.2008.05.057
Li C, et al. H5N1 Influenza Marker Vaccine for Serological Differentiation Between Vaccinated and Infected Chickens. Biochem Biophys Res Commun. 2008 Jul 25;372(2):293-7. PubMed PMID: 18501701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens. AU - Li,Chengjun, AU - Ping,Jihui, AU - Jing,Bo, AU - Deng,Guohua, AU - Jiang,Yongping, AU - Li,Yanbing, AU - Tian,Guobin, AU - Yu,Kangzhen, AU - Bu,Zhigao, AU - Chen,Hualan, Y1 - 2008/05/22/ PY - 2008/05/09/received PY - 2008/05/14/accepted PY - 2008/5/27/pubmed PY - 2008/8/13/medline PY - 2008/5/27/entrez SP - 293 EP - 7 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 372 IS - 2 N2 - Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18501701/H5N1_influenza_marker_vaccine_for_serological_differentiation_between_vaccinated_and_infected_chickens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)00975-3 DB - PRIME DP - Unbound Medicine ER -