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Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine.
Forensic Sci Int. 2008 Jul 04; 178(2-3):207-12.FS

Abstract

Quality assurance is one of the major issues in forensic analytical laboratories, where the need for a reference material (RM) has rapidly increased. RMs are very useful for method development and validation, internal quality control or proficiency tests. In the present study, we prepared a RM using drug-free hair for the determination of methamphetamine (MA) and its main metabolite, amphetamine (AP) according to the recommendations of ISO Guide 35. The concentrations of MA and AP were determined using two extraction methods, agitation with 1% HCl in methanol at 38 degrees C and ultrasonication with methanol/5M HCl (20:1), followed by gas chromatography/mass spectrometry (GC/MS) after derivatization with trifluoroacetic anhydride (TFAA). The assignment of values was conducted through the homogeneity study and characterization of the material. Furthermore, an internal proficiency test was performed with the prepared RM, of which the results were compared with those of the authentic hair RM prepared in our previous study. As a result, a hair RM containing MA and AP was prepared at the level of 4.86+/-0.69 ng/mg and 4.63+/-0.44 ng/mg, respectively. Most participants showed satisfactory performances in the internal proficiency test with the both RMs. The hair RM prepared in this study demonstrated its suitability for quality assurance in forensic laboratories.

Authors+Show Affiliations

National Institute of Scientific Investigation, 331-1 Sinwol-7-dong, Yangcheon-gu, Seoul 158-707, South Korea. spp26625@yahoo.co.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18502069

Citation

Lee, Sooyeun, et al. "Preparation and Application of a Fortified Hair Reference Material for the Determination of Methamphetamine and Amphetamine." Forensic Science International, vol. 178, no. 2-3, 2008, pp. 207-12.
Lee S, Park Y, Han E, et al. Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine. Forensic Sci Int. 2008;178(2-3):207-12.
Lee, S., Park, Y., Han, E., Choe, S., Lim, M., & Chung, H. (2008). Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine. Forensic Science International, 178(2-3), 207-12. https://doi.org/10.1016/j.forsciint.2008.04.005
Lee S, et al. Preparation and Application of a Fortified Hair Reference Material for the Determination of Methamphetamine and Amphetamine. Forensic Sci Int. 2008 Jul 4;178(2-3):207-12. PubMed PMID: 18502069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and application of a fortified hair reference material for the determination of methamphetamine and amphetamine. AU - Lee,Sooyeun, AU - Park,Yonghoon, AU - Han,Eunyoung, AU - Choe,Sanggil, AU - Lim,Miae, AU - Chung,Heesun, Y1 - 2008/05/23/ PY - 2008/02/04/received PY - 2008/04/03/revised PY - 2008/04/08/accepted PY - 2008/5/27/pubmed PY - 2008/7/11/medline PY - 2008/5/27/entrez SP - 207 EP - 12 JF - Forensic science international JO - Forensic Sci Int VL - 178 IS - 2-3 N2 - Quality assurance is one of the major issues in forensic analytical laboratories, where the need for a reference material (RM) has rapidly increased. RMs are very useful for method development and validation, internal quality control or proficiency tests. In the present study, we prepared a RM using drug-free hair for the determination of methamphetamine (MA) and its main metabolite, amphetamine (AP) according to the recommendations of ISO Guide 35. The concentrations of MA and AP were determined using two extraction methods, agitation with 1% HCl in methanol at 38 degrees C and ultrasonication with methanol/5M HCl (20:1), followed by gas chromatography/mass spectrometry (GC/MS) after derivatization with trifluoroacetic anhydride (TFAA). The assignment of values was conducted through the homogeneity study and characterization of the material. Furthermore, an internal proficiency test was performed with the prepared RM, of which the results were compared with those of the authentic hair RM prepared in our previous study. As a result, a hair RM containing MA and AP was prepared at the level of 4.86+/-0.69 ng/mg and 4.63+/-0.44 ng/mg, respectively. Most participants showed satisfactory performances in the internal proficiency test with the both RMs. The hair RM prepared in this study demonstrated its suitability for quality assurance in forensic laboratories. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/18502069/Preparation_and_application_of_a_fortified_hair_reference_material_for_the_determination_of_methamphetamine_and_amphetamine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(08)00167-9 DB - PRIME DP - Unbound Medicine ER -