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An interleukin-6 ZnO/SiO(2)/Si surface acoustic wave biosensor.
Biosens Bioelectron. 2008 Oct 15; 24(2):313-8.BB

Abstract

A novel high sensitivity ZnO/SiO(2)/Si Love mode surface acoustic wave (SAW) biosensor for the detection of interleukin-6 (IL-6), is reported. The biosensors operating at 747.7 MHz and 1.586 GHz were functionalized by immobilizing the monoclonal IL-6 antibody onto the ZnO biosensor surface both through direct surface adsorption and through covalent binding on gluteraldehyde. The morphology of the IL-6 antibody-protein complex was studied using scanning electron microscopy (SEM), and the mass of the IL-6 protein immobilized on the surface was measured from the frequency shift of the SAW resonator biosensor. The biosensor was shown to have extended linearity, which was observed to improve with higher sensor frequency and for IL-6 immobilization through the monoclonal antibody. Preliminary results of biosensor measurements of low levels of IL-6 in normal human serum are reported. The biosensor can be fully integrated with CMOS Si chips and developed as a portable real time detection system for the interleukin family of proteins in human serum.

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Authors+Show Affiliations

Krishnamoorthy S
Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA.
Iliadis AA
No affiliation info available
Bei T
No affiliation info available
Chrousos GP
No affiliation info available

MeSH

AcousticsAntibodies, MonoclonalBiosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayInterleukin-6Reproducibility of ResultsSensitivity and SpecificitySiliconSilicon DioxideTransducersZinc Oxide

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18502114

Citation

Krishnamoorthy, Soumya, et al. "An Interleukin-6 ZnO/SiO(2)/Si Surface Acoustic Wave Biosensor." Biosensors & Bioelectronics, vol. 24, no. 2, 2008, pp. 313-8.
Krishnamoorthy S, Iliadis AA, Bei T, et al. An interleukin-6 ZnO/SiO(2)/Si surface acoustic wave biosensor. Biosens Bioelectron. 2008;24(2):313-8.
Krishnamoorthy, S., Iliadis, A. A., Bei, T., & Chrousos, G. P. (2008). An interleukin-6 ZnO/SiO(2)/Si surface acoustic wave biosensor. Biosensors & Bioelectronics, 24(2), 313-8. https://doi.org/10.1016/j.bios.2008.04.011
Krishnamoorthy S, et al. An Interleukin-6 ZnO/SiO(2)/Si Surface Acoustic Wave Biosensor. Biosens Bioelectron. 2008 Oct 15;24(2):313-8. PubMed PMID: 18502114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An interleukin-6 ZnO/SiO(2)/Si surface acoustic wave biosensor. AU - Krishnamoorthy,Soumya, AU - Iliadis,Agis A, AU - Bei,Thaleia, AU - Chrousos,George P, Y1 - 2008/04/20/ PY - 2008/01/10/received PY - 2008/03/19/revised PY - 2008/04/03/accepted PY - 2008/5/27/pubmed PY - 2008/11/19/medline PY - 2008/5/27/entrez SP - 313 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 2 N2 - A novel high sensitivity ZnO/SiO(2)/Si Love mode surface acoustic wave (SAW) biosensor for the detection of interleukin-6 (IL-6), is reported. The biosensors operating at 747.7 MHz and 1.586 GHz were functionalized by immobilizing the monoclonal IL-6 antibody onto the ZnO biosensor surface both through direct surface adsorption and through covalent binding on gluteraldehyde. The morphology of the IL-6 antibody-protein complex was studied using scanning electron microscopy (SEM), and the mass of the IL-6 protein immobilized on the surface was measured from the frequency shift of the SAW resonator biosensor. The biosensor was shown to have extended linearity, which was observed to improve with higher sensor frequency and for IL-6 immobilization through the monoclonal antibody. Preliminary results of biosensor measurements of low levels of IL-6 in normal human serum are reported. The biosensor can be fully integrated with CMOS Si chips and developed as a portable real time detection system for the interleukin family of proteins in human serum. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18502114/An_interleukin_6_ZnO/SiO_2_/Si_surface_acoustic_wave_biosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00191-7 DB - PRIME DP - Unbound Medicine ER -