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Determination of alpha- and beta-amanitin in clinical urine samples by Capillary Zone Electrophoresis.
J Pharm Biomed Anal. 2008 Aug 05; 47(4-5):913-7.JP

Abstract

Amanitins are toxins found in species of the mushroom genera Amanita, Lepiota and Galerina. Intoxication after ingestion of these mushrooms can be fatal with an estimated 20% of mortality rate. An early diagnosis is necessary in order to avoid invasive and expensive therapy and to improve patient's prognosis. In this paper, a Capillary Zone Electrophoresis method was developed and validated to determine alpha- and beta-amanitin in urine in less than 7 min using 5 mM, pH 10 borate buffer as background electrolyte. The separation conditions were: capillary: 75 microm I.D., 41 cm effective length, 48 cm total length, 25 degrees C, 20 KV and PDA detection at 214 nm. Sample treatment for analysis only required urine dilution in background electrolyte. The method was validated following established criteria and was found to be selective, linear in the range 5-100 ng/ml. Intra- and inter-day precision and accuracy were within required limits. Limit of detection (LOD) and limit of quantification (LOQ) were 1.5 and 5 ng/ml, respectively. Eight urine samples from suspected cases of intoxication with amanitins were analyzed after 2 years of storage at -20 degrees C, and beta-amanitin was determined in two samples with concentrations of 53 and 65 ng/ml, respectively. The method here described includes the use of non-aggressive reagents to the capillary or the system and is the first Capillary Electrophoresis method used to determine amanitins in clinical samples.

Authors+Show Affiliations

División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas Dr. Ignacio Chávez, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18502601

Citation

Robinson-Fuentes, V A., et al. "Determination of Alpha- and Beta-amanitin in Clinical Urine Samples By Capillary Zone Electrophoresis." Journal of Pharmaceutical and Biomedical Analysis, vol. 47, no. 4-5, 2008, pp. 913-7.
Robinson-Fuentes VA, Jaime-Sánchez JL, García-Aguilar L, et al. Determination of alpha- and beta-amanitin in clinical urine samples by Capillary Zone Electrophoresis. J Pharm Biomed Anal. 2008;47(4-5):913-7.
Robinson-Fuentes, V. A., Jaime-Sánchez, J. L., García-Aguilar, L., Gómez-Peralta, M., Vázquez-Garcidueñas, M. S., & Vázquez-Marrufo, G. (2008). Determination of alpha- and beta-amanitin in clinical urine samples by Capillary Zone Electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 47(4-5), 913-7. https://doi.org/10.1016/j.jpba.2008.03.032
Robinson-Fuentes VA, et al. Determination of Alpha- and Beta-amanitin in Clinical Urine Samples By Capillary Zone Electrophoresis. J Pharm Biomed Anal. 2008 Aug 5;47(4-5):913-7. PubMed PMID: 18502601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of alpha- and beta-amanitin in clinical urine samples by Capillary Zone Electrophoresis. AU - Robinson-Fuentes,V A, AU - Jaime-Sánchez,J L, AU - García-Aguilar,L, AU - Gómez-Peralta,M, AU - Vázquez-Garcidueñas,M S, AU - Vázquez-Marrufo,G, Y1 - 2008/04/10/ PY - 2007/12/04/received PY - 2008/03/25/revised PY - 2008/03/26/accepted PY - 2008/5/27/pubmed PY - 2008/9/4/medline PY - 2008/5/27/entrez SP - 913 EP - 7 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 47 IS - 4-5 N2 - Amanitins are toxins found in species of the mushroom genera Amanita, Lepiota and Galerina. Intoxication after ingestion of these mushrooms can be fatal with an estimated 20% of mortality rate. An early diagnosis is necessary in order to avoid invasive and expensive therapy and to improve patient's prognosis. In this paper, a Capillary Zone Electrophoresis method was developed and validated to determine alpha- and beta-amanitin in urine in less than 7 min using 5 mM, pH 10 borate buffer as background electrolyte. The separation conditions were: capillary: 75 microm I.D., 41 cm effective length, 48 cm total length, 25 degrees C, 20 KV and PDA detection at 214 nm. Sample treatment for analysis only required urine dilution in background electrolyte. The method was validated following established criteria and was found to be selective, linear in the range 5-100 ng/ml. Intra- and inter-day precision and accuracy were within required limits. Limit of detection (LOD) and limit of quantification (LOQ) were 1.5 and 5 ng/ml, respectively. Eight urine samples from suspected cases of intoxication with amanitins were analyzed after 2 years of storage at -20 degrees C, and beta-amanitin was determined in two samples with concentrations of 53 and 65 ng/ml, respectively. The method here described includes the use of non-aggressive reagents to the capillary or the system and is the first Capillary Electrophoresis method used to determine amanitins in clinical samples. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18502601/Determination_of_alpha__and_beta_amanitin_in_clinical_urine_samples_by_Capillary_Zone_Electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00201-X DB - PRIME DP - Unbound Medicine ER -