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Identification of glucocorticoid- and cyclic AMP-responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes.
Biochem Biophys Res Commun. 1991 Apr 15; 176(1):385-91.BB

Abstract

Transcription of the gene coding for serine dehydratase (SDH, EC 4.2.1.13) in rat liver is induced 3-4 fold by glucocorticoids plus glucagon, but not by either hormone alone. For identification of the DNA elements mediating the glucocorticoid- and cyclic AMP-regulated expression of the SDH gene, primary cultures of adult rat hepatocytes were transfected with a fusion gene consisting of the 2.15 kb 5'-flanking sequence of the SDH gene linked to the coding sequence of the gene for chloramphenicol acetyltransferase (CAT). CAT assay demonstrated that transient expression of the SDH-CAT fusion gene was inducible by either dexamethasone or dibutyryl cyclic AMP, but that the effects of these inducers were not additive or synergistic. These results suggest that some structural organization of the DNA influences the hormonal actions in regulation of gene expression.

Authors+Show Affiliations

Matsuda K
Institute for Enzyme Research, University of Tokushima, Japan.
Noda C
No affiliation info available
Fukushima C
No affiliation info available
Ichihara A
No affiliation info available

MeSH

AnimalsBase SequenceBinding SitesBucladesineCarcinoma, HepatocellularCell LineCells, CulturedChloramphenicolChloramphenicol O-AcetyltransferaseCyclic AMPDexamethasoneGenesHumansL-Serine DehydrataseLiverLiver NeoplasmsMaleMolecular Sequence DataRatsRats, Inbred StrainsRecombinant Fusion ProteinsTransfection

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1850265

Citation

Matsuda, K, et al. "Identification of Glucocorticoid- and Cyclic AMP-responsive Elements of the Rat Serine Dehydratase Gene: Difference in Responses of the Transfected and Chromosomal Genes." Biochemical and Biophysical Research Communications, vol. 176, no. 1, 1991, pp. 385-91.
Matsuda K, Noda C, Fukushima C, et al. Identification of glucocorticoid- and cyclic AMP-responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes. Biochem Biophys Res Commun. 1991;176(1):385-91.
Matsuda, K., Noda, C., Fukushima, C., & Ichihara, A. (1991). Identification of glucocorticoid- and cyclic AMP-responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes. Biochemical and Biophysical Research Communications, 176(1), 385-91.
Matsuda K, et al. Identification of Glucocorticoid- and Cyclic AMP-responsive Elements of the Rat Serine Dehydratase Gene: Difference in Responses of the Transfected and Chromosomal Genes. Biochem Biophys Res Commun. 1991 Apr 15;176(1):385-91. PubMed PMID: 1850265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of glucocorticoid- and cyclic AMP-responsive elements of the rat serine dehydratase gene: difference in responses of the transfected and chromosomal genes. AU - Matsuda,K, AU - Noda,C, AU - Fukushima,C, AU - Ichihara,A, PY - 1991/4/15/pubmed PY - 1991/4/15/medline PY - 1991/4/15/entrez SP - 385 EP - 91 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 176 IS - 1 N2 - Transcription of the gene coding for serine dehydratase (SDH, EC 4.2.1.13) in rat liver is induced 3-4 fold by glucocorticoids plus glucagon, but not by either hormone alone. For identification of the DNA elements mediating the glucocorticoid- and cyclic AMP-regulated expression of the SDH gene, primary cultures of adult rat hepatocytes were transfected with a fusion gene consisting of the 2.15 kb 5'-flanking sequence of the SDH gene linked to the coding sequence of the gene for chloramphenicol acetyltransferase (CAT). CAT assay demonstrated that transient expression of the SDH-CAT fusion gene was inducible by either dexamethasone or dibutyryl cyclic AMP, but that the effects of these inducers were not additive or synergistic. These results suggest that some structural organization of the DNA influences the hormonal actions in regulation of gene expression. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/1850265/Identification_of_glucocorticoid__and_cyclic_AMP_responsive_elements_of_the_rat_serine_dehydratase_gene:_difference_in_responses_of_the_transfected_and_chromosomal_genes_ DB - PRIME DP - Unbound Medicine ER -