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Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil.
J Clin Virol. 2008 Sep; 43(1):60-5.JC

Abstract

BACKGROUND

Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations.

OBJECTIVES

To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations.

STUDY DESIGN

A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction.

RESULTS

B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products.

CONCLUSION

We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.

Authors+Show Affiliations

Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Rodovia BR 316-Km 7, S/N Levilândia, 67037-000 Ananindeua, Pará, Brazil. ronaldofreitas@iec.pa.gov.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18502682

Citation

Freitas, Ronaldo B., et al. "Molecular Characterization of Human Erythrovirus B19 Strains Obtained From Patients With Several Clinical Presentations in the Amazon Region of Brazil." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 43, no. 1, 2008, pp. 60-5.
Freitas RB, Melo FL, Oliveira DS, et al. Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. J Clin Virol. 2008;43(1):60-5.
Freitas, R. B., Melo, F. L., Oliveira, D. S., Romano, C. M., Freitas, M. R., Macêdo, O., Linhares, A. C., de A Zanotto, P. M., & Durigon, E. L. (2008). Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 43(1), 60-5. https://doi.org/10.1016/j.jcv.2008.03.033
Freitas RB, et al. Molecular Characterization of Human Erythrovirus B19 Strains Obtained From Patients With Several Clinical Presentations in the Amazon Region of Brazil. J Clin Virol. 2008;43(1):60-5. PubMed PMID: 18502682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. AU - Freitas,Ronaldo B, AU - Melo,Fernando L, AU - Oliveira,Darleise S, AU - Romano,Camila M, AU - Freitas,Maria Rute C, AU - Macêdo,Olinda, AU - Linhares,Alexandre C, AU - de A Zanotto,Paolo M, AU - Durigon,Edison Luiz, Y1 - 2008/05/27/ PY - 2007/12/19/received PY - 2008/03/14/revised PY - 2008/03/26/accepted PY - 2008/5/27/pubmed PY - 2008/11/5/medline PY - 2008/5/27/entrez SP - 60 EP - 5 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 43 IS - 1 N2 - BACKGROUND: Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations. OBJECTIVES: To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations. STUDY DESIGN: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction. RESULTS: B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products. CONCLUSION: We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/18502682/Molecular_characterization_of_human_erythrovirus_B19_strains_obtained_from_patients_with_several_clinical_presentations_in_the_Amazon_region_of_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(08)00131-5 DB - PRIME DP - Unbound Medicine ER -