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Brain regional differences in the effect of ethanol on GABA release from presynaptic terminals.
J Pharmacol Exp Ther. 2008 Aug; 326(2):596-603.JP

Abstract

Whereas ethanol has behavioral actions consistent with increased GABAergic function, attempts to demonstrate a direct enhancement of GABA-gated currents by ethanol have produced mixed results. Recent work has suggested that a part of the GABAergic profile of ethanol may result from enhanced GABA release from presynaptic terminals. The present study examines the effect of ethanol on GABA release in several brain regions to assess the regional nature of ethanol-induced GABA release. Whole-cell voltage clamp recording of spontaneous inhibitory postsynaptic currents (sIPSCs) from mechanically dissociated neurons and miniature inhibitory postsynaptic currents (mIPSCs) and paired-pulse ratio (PPR) from a slice preparation were used to quantify GABA release. Ethanol produced a concentration-dependent increase in the frequency of sIPSCs recorded from mechanically dissociated cerebellar Purkinje neurons and mIPSCs from substantia nigra neurons without having an effect on sIPSCs recorded from lateral septal or cerebrocortical neurons. This regional difference in the effect of ethanol on GABA release was confirmed with PPR recording from brain slices. These data indicate that ethanol can act on presynaptic terminals to increase GABA release in some brain regions while having little or no effect on GABA release in others. This regional difference is consistent with earlier in vivo studies in which ethanol affected neural activity and sensitivity to GABA in some, but not all, brain sites.

Authors+Show Affiliations

Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, NC 27599, USA. hec@med.unc.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18502983

Citation

Criswell, Hugh E., et al. "Brain Regional Differences in the Effect of Ethanol On GABA Release From Presynaptic Terminals." The Journal of Pharmacology and Experimental Therapeutics, vol. 326, no. 2, 2008, pp. 596-603.
Criswell HE, Ming Z, Kelm MK, et al. Brain regional differences in the effect of ethanol on GABA release from presynaptic terminals. J Pharmacol Exp Ther. 2008;326(2):596-603.
Criswell, H. E., Ming, Z., Kelm, M. K., & Breese, G. R. (2008). Brain regional differences in the effect of ethanol on GABA release from presynaptic terminals. The Journal of Pharmacology and Experimental Therapeutics, 326(2), 596-603. https://doi.org/10.1124/jpet.107.135418
Criswell HE, et al. Brain Regional Differences in the Effect of Ethanol On GABA Release From Presynaptic Terminals. J Pharmacol Exp Ther. 2008;326(2):596-603. PubMed PMID: 18502983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain regional differences in the effect of ethanol on GABA release from presynaptic terminals. AU - Criswell,Hugh E, AU - Ming,Zhen, AU - Kelm,M Katherine, AU - Breese,George R, Y1 - 2008/05/23/ PY - 2008/5/27/pubmed PY - 2008/8/13/medline PY - 2008/5/27/entrez SP - 596 EP - 603 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 326 IS - 2 N2 - Whereas ethanol has behavioral actions consistent with increased GABAergic function, attempts to demonstrate a direct enhancement of GABA-gated currents by ethanol have produced mixed results. Recent work has suggested that a part of the GABAergic profile of ethanol may result from enhanced GABA release from presynaptic terminals. The present study examines the effect of ethanol on GABA release in several brain regions to assess the regional nature of ethanol-induced GABA release. Whole-cell voltage clamp recording of spontaneous inhibitory postsynaptic currents (sIPSCs) from mechanically dissociated neurons and miniature inhibitory postsynaptic currents (mIPSCs) and paired-pulse ratio (PPR) from a slice preparation were used to quantify GABA release. Ethanol produced a concentration-dependent increase in the frequency of sIPSCs recorded from mechanically dissociated cerebellar Purkinje neurons and mIPSCs from substantia nigra neurons without having an effect on sIPSCs recorded from lateral septal or cerebrocortical neurons. This regional difference in the effect of ethanol on GABA release was confirmed with PPR recording from brain slices. These data indicate that ethanol can act on presynaptic terminals to increase GABA release in some brain regions while having little or no effect on GABA release in others. This regional difference is consistent with earlier in vivo studies in which ethanol affected neural activity and sensitivity to GABA in some, but not all, brain sites. SN - 1521-0103 UR - https://www.unboundmedicine.com/medline/citation/18502983/Brain_regional_differences_in_the_effect_of_ethanol_on_GABA_release_from_presynaptic_terminals_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=18502983 DB - PRIME DP - Unbound Medicine ER -