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Guided tissue regeneration may modulate gene expression in periodontal intrabony defects: a human study.
J Periodontal Res 2008; 43(4):459-64JP

Abstract

BACKGROUND AND OBJECTIVE

Guided tissue regeneration has been shown to lead to periodontal regeneration, however, the mechanisms involved remain to be clarified. The present study was carried out to assess the expression of genes involved in the healing process of periodontal tissues in membrane-protected vs. nonprotected intrabony defects in humans.

MATERIAL AND METHODS

Thirty patients with deep intrabony defects (> or = 5 mm, two or three walls) around teeth that were scheduled for extraction were selected and randomly assigned to receive one of the following treatments: flap surgery alone (control group) or flap surgery plus guided tissue regeneration (expanded polytetrafluorethylene (e-PTFE) membrane) (test group). Twenty-one days later, the newly formed tissue was harvested and quantitatively assessed using the polymerase chain reaction assay for the expression of the following genes: alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, osteocalcin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-4, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase-9.

RESULTS

Data analysis demonstrated that mRNA levels for alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase -9 were higher in the sites where guided tissue regeneration was applied compared with the control sites (p < 0.05), whereas osteocalcin mRNA levels were lower (p < 0.05). No difference was observed in interleukin-4 mRNA levels between control and test groups.

CONCLUSION

Within the limits of this study, it can be concluded that genes are differentially expressed in membrane barrier-led periodontal healing when compared with flap surgery alone, and this may account for the clinical outcome achieved by guided tissue regeneration.

Authors+Show Affiliations

Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18503515

Citation

Lima, L L., et al. "Guided Tissue Regeneration May Modulate Gene Expression in Periodontal Intrabony Defects: a Human Study." Journal of Periodontal Research, vol. 43, no. 4, 2008, pp. 459-64.
Lima LL, Gonçalves PF, Sallum EA, et al. Guided tissue regeneration may modulate gene expression in periodontal intrabony defects: a human study. J Periodont Res. 2008;43(4):459-64.
Lima, L. L., Gonçalves, P. F., Sallum, E. A., Casati, M. Z., & Nociti, F. H. (2008). Guided tissue regeneration may modulate gene expression in periodontal intrabony defects: a human study. Journal of Periodontal Research, 43(4), pp. 459-64. doi:10.1111/j.1600-0765.2008.01094.x.
Lima LL, et al. Guided Tissue Regeneration May Modulate Gene Expression in Periodontal Intrabony Defects: a Human Study. J Periodont Res. 2008;43(4):459-64. PubMed PMID: 18503515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Guided tissue regeneration may modulate gene expression in periodontal intrabony defects: a human study. AU - Lima,L L, AU - Gonçalves,P F, AU - Sallum,E A, AU - Casati,M Z, AU - Nociti,F H,Jr PY - 2008/5/28/pubmed PY - 2008/11/19/medline PY - 2008/5/28/entrez SP - 459 EP - 64 JF - Journal of periodontal research JO - J. Periodont. Res. VL - 43 IS - 4 N2 - BACKGROUND AND OBJECTIVE: Guided tissue regeneration has been shown to lead to periodontal regeneration, however, the mechanisms involved remain to be clarified. The present study was carried out to assess the expression of genes involved in the healing process of periodontal tissues in membrane-protected vs. nonprotected intrabony defects in humans. MATERIAL AND METHODS: Thirty patients with deep intrabony defects (> or = 5 mm, two or three walls) around teeth that were scheduled for extraction were selected and randomly assigned to receive one of the following treatments: flap surgery alone (control group) or flap surgery plus guided tissue regeneration (expanded polytetrafluorethylene (e-PTFE) membrane) (test group). Twenty-one days later, the newly formed tissue was harvested and quantitatively assessed using the polymerase chain reaction assay for the expression of the following genes: alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, osteocalcin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-4, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase-9. RESULTS: Data analysis demonstrated that mRNA levels for alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase -9 were higher in the sites where guided tissue regeneration was applied compared with the control sites (p < 0.05), whereas osteocalcin mRNA levels were lower (p < 0.05). No difference was observed in interleukin-4 mRNA levels between control and test groups. CONCLUSION: Within the limits of this study, it can be concluded that genes are differentially expressed in membrane barrier-led periodontal healing when compared with flap surgery alone, and this may account for the clinical outcome achieved by guided tissue regeneration. SN - 1600-0765 UR - https://www.unboundmedicine.com/medline/citation/18503515/Guided_tissue_regeneration_may_modulate_gene_expression_in_periodontal_intrabony_defects:_a_human_study_ L2 - https://doi.org/10.1111/j.1600-0765.2008.01094.x DB - PRIME DP - Unbound Medicine ER -