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[Effects of Radix salviae miltiorrhizae on reperfusion injury and hepatocyte apoptosis in donor liver after orthotopic liver transplantation in rats].
Nan Fang Yi Ke Da Xue Xue Bao. 2008 May; 28(5):870-3.NF

Abstract

OBJECTIVE

To investigate the effect of Radix salviae miltiorrhizae as a cold reperfusion solution on donor liver ischemia-reperfusion injury and hepatocyte apoptosis in rat models of orthotopic liver transplantation.

METHODS

Forty male SD rats were randomly divided into miltiorrhizae group, control group and sham operation group. For cold preservation of the graft, miltiorrhizae-containing Ringer's lactate solution was used as the reperfusion and preservation solution in the miltiorrhizae group, and only Ringer's lactate solution was used in the control group. All the grafts were preserved for 5 h before orthotopic transplantation. Six hours after graft reperfusion, the recipient rats were sacrificed, and the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured, and the changes of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the liver graft were observed. The hepatocyte apoptosis was detected using TUNEL, with the histopathological changes observed under light and electron microscopes.

RESULTS

Serum ALT and AST levels were significantly lower in miltiorrhizae group than in the control group after graft reperfusion (P<0.01). Compared with the sham operation group, the apoptotic index and MDA levels in the graft was significantly decreased, while SOD and GSH-PX levels increased in miltiorrhizae group (P<0.01). Histopathological changes were obviously milder in miltiorrhizae group than in the sham operation group.

CONCLUSION

Radix salviae miltiorrhizae can protect the hepatocytes from cold preservation and reperfusion-induced apoptosis and significantly alleviates ischemia-reperfusion injury in rat liver graft after orthotopic transplantation.

Authors+Show Affiliations

Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. wangyu@fimmu.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18504224

Citation

Wang, Yu, et al. "[Effects of Radix Salviae Miltiorrhizae On Reperfusion Injury and Hepatocyte Apoptosis in Donor Liver After Orthotopic Liver Transplantation in Rats]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 28, no. 5, 2008, pp. 870-3.
Wang Y, Zhou J, Jiang XQ. [Effects of Radix salviae miltiorrhizae on reperfusion injury and hepatocyte apoptosis in donor liver after orthotopic liver transplantation in rats]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(5):870-3.
Wang, Y., Zhou, J., & Jiang, X. Q. (2008). [Effects of Radix salviae miltiorrhizae on reperfusion injury and hepatocyte apoptosis in donor liver after orthotopic liver transplantation in rats]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 28(5), 870-3.
Wang Y, Zhou J, Jiang XQ. [Effects of Radix Salviae Miltiorrhizae On Reperfusion Injury and Hepatocyte Apoptosis in Donor Liver After Orthotopic Liver Transplantation in Rats]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(5):870-3. PubMed PMID: 18504224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Radix salviae miltiorrhizae on reperfusion injury and hepatocyte apoptosis in donor liver after orthotopic liver transplantation in rats]. AU - Wang,Yu, AU - Zhou,Jie, AU - Jiang,Xiao-qing, PY - 2008/5/28/pubmed PY - 2009/4/14/medline PY - 2008/5/28/entrez SP - 870 EP - 3 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 28 IS - 5 N2 - OBJECTIVE: To investigate the effect of Radix salviae miltiorrhizae as a cold reperfusion solution on donor liver ischemia-reperfusion injury and hepatocyte apoptosis in rat models of orthotopic liver transplantation. METHODS: Forty male SD rats were randomly divided into miltiorrhizae group, control group and sham operation group. For cold preservation of the graft, miltiorrhizae-containing Ringer's lactate solution was used as the reperfusion and preservation solution in the miltiorrhizae group, and only Ringer's lactate solution was used in the control group. All the grafts were preserved for 5 h before orthotopic transplantation. Six hours after graft reperfusion, the recipient rats were sacrificed, and the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured, and the changes of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the liver graft were observed. The hepatocyte apoptosis was detected using TUNEL, with the histopathological changes observed under light and electron microscopes. RESULTS: Serum ALT and AST levels were significantly lower in miltiorrhizae group than in the control group after graft reperfusion (P<0.01). Compared with the sham operation group, the apoptotic index and MDA levels in the graft was significantly decreased, while SOD and GSH-PX levels increased in miltiorrhizae group (P<0.01). Histopathological changes were obviously milder in miltiorrhizae group than in the sham operation group. CONCLUSION: Radix salviae miltiorrhizae can protect the hepatocytes from cold preservation and reperfusion-induced apoptosis and significantly alleviates ischemia-reperfusion injury in rat liver graft after orthotopic transplantation. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/18504224/[Effects_of_Radix_salviae_miltiorrhizae_on_reperfusion_injury_and_hepatocyte_apoptosis_in_donor_liver_after_orthotopic_liver_transplantation_in_rats]_ L2 - https://medlineplus.gov/livertransplantation.html DB - PRIME DP - Unbound Medicine ER -