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A study of chemical composition and antimicrobial activity of Bulgarian propolis.
Folia Med (Plovdiv) 2007; 49(3-4):63-9FM

Abstract

Propolis is a natural product which exhibits pronounced antimicrobial and anti-inflammatory effects.

AIM

To analyze the chemical composition and antimicrobial effect of Bulgarian propolis, p-cumaric acid and ferulic acid on certain Gram-positive and Gram-negative microorganisms.

MATERIAL AND METHODS

Ethanol extract of Bulgarian propolis (Propolin) and water extract of Bulgarian propolis (Propolstom), ferulic acid and p-cumaric acid were used. The chemical HPLC-analysis of propolis was performed with Varian Prostar system. Microbiological tests were done on isolates of Streptococcus beta-haemolyticus, Enterococcus faecalis, Candida albicans and Escherichia coli. Non-treated and ethanol-treated cultures of the corresponding strains were used as controls. Minimal bactericidal and bacteriostatic concentration of Propolin were determined by agar method.

RESULTS

The concentration of p-cumaric acid and ferulic acid in ethanol extract of propolis is about 1%. The minimal bactericidal concentration of Bulgarian propolis against Streptococcus beta-haemolyticus is 281 +/- 89.3 microg/ml, against Enterococcus faecalis--3490 +/- 633.1 microg/ml, against Candida albicans--1375 +/- 255.2 microg/ml, and against Escherichia coli--24306 +/- 1706.4 microg/ml. The minimal bactericidal concentration of p-cumaric acid and ferulic acid against Streptococcus beta-haemolyticus is 212 +/- 6.5 microg/ml and 208 +/- 7.7 microg/ml, against Enterococcus faecalis--3013 +/- 176.6 and 3032 +/- 203.4 microg/ml, against Candida albicans--157 +/- 15.5 microg/ml and 149 +/- 23.2 microg/ml, and against Escherichia coli--798 +/- 7.7 microg/ml and 801 +/- 22.8 microg/ml, respectively.

CONCLUSIONS

The phenolic compounds p-cumaric acid and ferulic acid are 10 times less extracted in water extraction than in ethanol extraction. The most susceptible strains to the Bulgarian propolis and phenolic compounds are Streptococcus beta-haemolyticus and Candida albicans and the least affected are Escherichia coli and Enterococcus faecalis.

Authors+Show Affiliations

Department of Microbiology and Immunology, ' Medical University, Plovdiv, Bulgaria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18504937

Citation

Gardjeva, Petya A., et al. "A Study of Chemical Composition and Antimicrobial Activity of Bulgarian Propolis." Folia Medica, vol. 49, no. 3-4, 2007, pp. 63-9.
Gardjeva PA, Dimitrova SZ, Kostadinov ID, et al. A study of chemical composition and antimicrobial activity of Bulgarian propolis. Folia Med (Plovdiv). 2007;49(3-4):63-9.
Gardjeva, P. A., Dimitrova, S. Z., Kostadinov, I. D., Murdjeva, M. A., Peyche, L. P., Lukanov, L. K., ... Alexandrov, A. S. (2007). A study of chemical composition and antimicrobial activity of Bulgarian propolis. Folia Medica, 49(3-4), pp. 63-9.
Gardjeva PA, et al. A Study of Chemical Composition and Antimicrobial Activity of Bulgarian Propolis. Folia Med (Plovdiv). 2007;49(3-4):63-9. PubMed PMID: 18504937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A study of chemical composition and antimicrobial activity of Bulgarian propolis. AU - Gardjeva,Petya A, AU - Dimitrova,Stela Z, AU - Kostadinov,Iliya D, AU - Murdjeva,Marianna A, AU - Peyche,Lyudmil P, AU - Lukanov,Lyudmil K, AU - Stanimirova,Irina V, AU - Alexandrov,Alexandar S, PY - 2008/5/29/pubmed PY - 2008/7/2/medline PY - 2008/5/29/entrez SP - 63 EP - 9 JF - Folia medica JO - Folia Med (Plovdiv) VL - 49 IS - 3-4 N2 - UNLABELLED: Propolis is a natural product which exhibits pronounced antimicrobial and anti-inflammatory effects. AIM: To analyze the chemical composition and antimicrobial effect of Bulgarian propolis, p-cumaric acid and ferulic acid on certain Gram-positive and Gram-negative microorganisms. MATERIAL AND METHODS: Ethanol extract of Bulgarian propolis (Propolin) and water extract of Bulgarian propolis (Propolstom), ferulic acid and p-cumaric acid were used. The chemical HPLC-analysis of propolis was performed with Varian Prostar system. Microbiological tests were done on isolates of Streptococcus beta-haemolyticus, Enterococcus faecalis, Candida albicans and Escherichia coli. Non-treated and ethanol-treated cultures of the corresponding strains were used as controls. Minimal bactericidal and bacteriostatic concentration of Propolin were determined by agar method. RESULTS: The concentration of p-cumaric acid and ferulic acid in ethanol extract of propolis is about 1%. The minimal bactericidal concentration of Bulgarian propolis against Streptococcus beta-haemolyticus is 281 +/- 89.3 microg/ml, against Enterococcus faecalis--3490 +/- 633.1 microg/ml, against Candida albicans--1375 +/- 255.2 microg/ml, and against Escherichia coli--24306 +/- 1706.4 microg/ml. The minimal bactericidal concentration of p-cumaric acid and ferulic acid against Streptococcus beta-haemolyticus is 212 +/- 6.5 microg/ml and 208 +/- 7.7 microg/ml, against Enterococcus faecalis--3013 +/- 176.6 and 3032 +/- 203.4 microg/ml, against Candida albicans--157 +/- 15.5 microg/ml and 149 +/- 23.2 microg/ml, and against Escherichia coli--798 +/- 7.7 microg/ml and 801 +/- 22.8 microg/ml, respectively. CONCLUSIONS: The phenolic compounds p-cumaric acid and ferulic acid are 10 times less extracted in water extraction than in ethanol extraction. The most susceptible strains to the Bulgarian propolis and phenolic compounds are Streptococcus beta-haemolyticus and Candida albicans and the least affected are Escherichia coli and Enterococcus faecalis. SN - 0204-8043 UR - https://www.unboundmedicine.com/medline/citation/18504937/A_study_of_chemical_composition_and_antimicrobial_activity_of_Bulgarian_propolis_ DB - PRIME DP - Unbound Medicine ER -