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Ion-trap versus time-of-flight mass spectrometry coupled to capillary electrophoresis to analyze biogenic amines in wine.
J Chromatogr A. 2008 Jun 27; 1195(1-2):150-6.JC

Abstract

In this work, two different capillary electrophoresis-mass spectrometry (CE-MS) methods, namely, capillary electrophoresis-ion-trap mass spectrometry (CE-IT-MS) and capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS), applied to analyze biogenic amines in wine samples are investigated. A group of five amines was selected as case study (namely, putrescine, cadaverine, histamine, phenylethylamine and tyramine) since they are the most frequently biogenic amines found in wines. The possibilities of both instruments in terms of sensitivity, selectivity and quantitation during the determination of the mentioned biogenic amines in wine samples was studied and their results corroborated by HPLC. After optimization of the analytical conditions, CE-IT-MS and CE-TOF-MS allowed the identification of biogenic amines in wines without any previous treatment except diluting 1:1 with water and filtering. Biogenic amines were determined in three red wines and one white wine showing, as expected, a higher concentration in red wines. Moreover, CE-IT-MS and CE-TOF-MS were compared regarding their capacity to detect other biogenic amines different to the selected ones in wine samples, showing CE-TOF-MS a much better capability (i.e., putrescine, cadaverine, histamine, phenylethylamine, tyramine, triptamine, spermidine, spermine, ethanolamine and isoamylamine were identified by CE-TOF-MS in a single analysis). Moreover, CE-TOF-MS allowed the quantitation of biogenic amines with limits of detection as low as 10 ng/mL, comparable to those obtained using HPLC with fluorescence detection, without any previous derivatization step and with analysis times fivefold faster (40 min by HPLC and 8 min by CE-TOF-MS).

Authors+Show Affiliations

Institute of Industrial Fermentations (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18508068

Citation

Simó, Carolina, et al. "Ion-trap Versus Time-of-flight Mass Spectrometry Coupled to Capillary Electrophoresis to Analyze Biogenic Amines in Wine." Journal of Chromatography. A, vol. 1195, no. 1-2, 2008, pp. 150-6.
Simó C, Moreno-Arribas MV, Cifuentes A. Ion-trap versus time-of-flight mass spectrometry coupled to capillary electrophoresis to analyze biogenic amines in wine. J Chromatogr A. 2008;1195(1-2):150-6.
Simó, C., Moreno-Arribas, M. V., & Cifuentes, A. (2008). Ion-trap versus time-of-flight mass spectrometry coupled to capillary electrophoresis to analyze biogenic amines in wine. Journal of Chromatography. A, 1195(1-2), 150-6. https://doi.org/10.1016/j.chroma.2008.05.004
Simó C, Moreno-Arribas MV, Cifuentes A. Ion-trap Versus Time-of-flight Mass Spectrometry Coupled to Capillary Electrophoresis to Analyze Biogenic Amines in Wine. J Chromatogr A. 2008 Jun 27;1195(1-2):150-6. PubMed PMID: 18508068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ion-trap versus time-of-flight mass spectrometry coupled to capillary electrophoresis to analyze biogenic amines in wine. AU - Simó,Carolina, AU - Moreno-Arribas,M Victoria, AU - Cifuentes,Alejandro, Y1 - 2008/05/10/ PY - 2008/02/25/received PY - 2008/04/28/revised PY - 2008/05/07/accepted PY - 2008/5/30/pubmed PY - 2008/9/3/medline PY - 2008/5/30/entrez SP - 150 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1195 IS - 1-2 N2 - In this work, two different capillary electrophoresis-mass spectrometry (CE-MS) methods, namely, capillary electrophoresis-ion-trap mass spectrometry (CE-IT-MS) and capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS), applied to analyze biogenic amines in wine samples are investigated. A group of five amines was selected as case study (namely, putrescine, cadaverine, histamine, phenylethylamine and tyramine) since they are the most frequently biogenic amines found in wines. The possibilities of both instruments in terms of sensitivity, selectivity and quantitation during the determination of the mentioned biogenic amines in wine samples was studied and their results corroborated by HPLC. After optimization of the analytical conditions, CE-IT-MS and CE-TOF-MS allowed the identification of biogenic amines in wines without any previous treatment except diluting 1:1 with water and filtering. Biogenic amines were determined in three red wines and one white wine showing, as expected, a higher concentration in red wines. Moreover, CE-IT-MS and CE-TOF-MS were compared regarding their capacity to detect other biogenic amines different to the selected ones in wine samples, showing CE-TOF-MS a much better capability (i.e., putrescine, cadaverine, histamine, phenylethylamine, tyramine, triptamine, spermidine, spermine, ethanolamine and isoamylamine were identified by CE-TOF-MS in a single analysis). Moreover, CE-TOF-MS allowed the quantitation of biogenic amines with limits of detection as low as 10 ng/mL, comparable to those obtained using HPLC with fluorescence detection, without any previous derivatization step and with analysis times fivefold faster (40 min by HPLC and 8 min by CE-TOF-MS). SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18508068/Ion_trap_versus_time_of_flight_mass_spectrometry_coupled_to_capillary_electrophoresis_to_analyze_biogenic_amines_in_wine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00831-5 DB - PRIME DP - Unbound Medicine ER -