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Hyperpolarization-activated cyclic nucleotide-gated channels in mouse vomeronasal sensory neurons.
J Neurophysiol. 2008 Aug; 100(2):576-86.JN

Abstract

Hyperpolarization-activated currents (Ih) are present in several neurons of the central and peripheral nervous system. However, Ih in neurons of the vomeronasal organ (VNO) is not well characterized. We studied the properties of Ih in sensory neurons from acute slices of mouse VNO. In voltage-clamp studies, Ih was identified by the characteristic kinetics of activation, voltage dependence, and blockage by Cs+ or ZD-7288, two blockers of the Ih. Forskolin, an activator of adenylyl cyclase, shifted the activation curve for Ih to less negative potentials. A comparison of Ih properties in VNO neurons with those of heterologously expressed hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, together with RT-PCR experiments in VNO, indicate that Ih is caused by HCN2 and/or HCN4 subunits. In current-clamp recordings, blocking Ih with ZD-7288 induced a hyperpolarization of 5.1 mV, an increase in input resistance, a decrease in the sensitivity to elicit action potentials in response to small current injections, and did not modify the frequency of action potentials elicited by a large current injection. It has been shown that in VNO neurons some pheromones induce a decrease in cAMP concentration, but the physiological role of cAMP is unknown. After application of blockers of adenylyl cyclase, we measured a hyperpolarization of 5.1 mV in 11 of 14 neurons, suggesting that basal levels of cAMP could modulate the resting potential. In conclusion, these results show that mouse VNO neurons express HCN2 and/or HCN4 subunits and that Ih contributes to setting the resting membrane potential and to increase excitability at stimulus threshold.

Authors+Show Affiliations

Sector of Neurobiology, International School for Advanced Studies, Scuola Internazionale di Studi Superiori Avanzati, Neurobiology Sector, Via Beirut 2-4, 34014 Trieste, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18509074

Citation

Dibattista, Michele, et al. "Hyperpolarization-activated Cyclic Nucleotide-gated Channels in Mouse Vomeronasal Sensory Neurons." Journal of Neurophysiology, vol. 100, no. 2, 2008, pp. 576-86.
Dibattista M, Mazzatenta A, Grassi F, et al. Hyperpolarization-activated cyclic nucleotide-gated channels in mouse vomeronasal sensory neurons. J Neurophysiol. 2008;100(2):576-86.
Dibattista, M., Mazzatenta, A., Grassi, F., Tirindelli, R., & Menini, A. (2008). Hyperpolarization-activated cyclic nucleotide-gated channels in mouse vomeronasal sensory neurons. Journal of Neurophysiology, 100(2), 576-86. https://doi.org/10.1152/jn.90263.2008
Dibattista M, et al. Hyperpolarization-activated Cyclic Nucleotide-gated Channels in Mouse Vomeronasal Sensory Neurons. J Neurophysiol. 2008;100(2):576-86. PubMed PMID: 18509074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hyperpolarization-activated cyclic nucleotide-gated channels in mouse vomeronasal sensory neurons. AU - Dibattista,Michele, AU - Mazzatenta,Andrea, AU - Grassi,Francesca, AU - Tirindelli,Roberto, AU - Menini,Anna, Y1 - 2008/05/28/ PY - 2008/5/30/pubmed PY - 2008/11/6/medline PY - 2008/5/30/entrez SP - 576 EP - 86 JF - Journal of neurophysiology JO - J Neurophysiol VL - 100 IS - 2 N2 - Hyperpolarization-activated currents (Ih) are present in several neurons of the central and peripheral nervous system. However, Ih in neurons of the vomeronasal organ (VNO) is not well characterized. We studied the properties of Ih in sensory neurons from acute slices of mouse VNO. In voltage-clamp studies, Ih was identified by the characteristic kinetics of activation, voltage dependence, and blockage by Cs+ or ZD-7288, two blockers of the Ih. Forskolin, an activator of adenylyl cyclase, shifted the activation curve for Ih to less negative potentials. A comparison of Ih properties in VNO neurons with those of heterologously expressed hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, together with RT-PCR experiments in VNO, indicate that Ih is caused by HCN2 and/or HCN4 subunits. In current-clamp recordings, blocking Ih with ZD-7288 induced a hyperpolarization of 5.1 mV, an increase in input resistance, a decrease in the sensitivity to elicit action potentials in response to small current injections, and did not modify the frequency of action potentials elicited by a large current injection. It has been shown that in VNO neurons some pheromones induce a decrease in cAMP concentration, but the physiological role of cAMP is unknown. After application of blockers of adenylyl cyclase, we measured a hyperpolarization of 5.1 mV in 11 of 14 neurons, suggesting that basal levels of cAMP could modulate the resting potential. In conclusion, these results show that mouse VNO neurons express HCN2 and/or HCN4 subunits and that Ih contributes to setting the resting membrane potential and to increase excitability at stimulus threshold. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/18509074/Hyperpolarization_activated_cyclic_nucleotide_gated_channels_in_mouse_vomeronasal_sensory_neurons_ L2 - https://journals.physiology.org/doi/10.1152/jn.90263.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -