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Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae).
Insect Biochem Mol Biol. 2008 Jun; 38(6):634-44.IB

Abstract

The two most damaging biotypes of Bemisia tabaci, B and Q, have both evolved strong resistance to the neonicotinoid insecticide imidacloprid. The major mechanism in all samples investigated so far appeared to be enhanced detoxification by cytochrome P450s monooxygenases (P450s). In this study, a polymerase chain reaction (PCR) technology using degenerate primers based on conserved P450 helix I and heme-binding regions was employed to identify P450 cDNA sequences in B. tabaci that might be involved in imidacloprid resistance. Eleven distinct P450 cDNA sequences were isolated and classified as members of the CYP4 or CYP6 families. The mRNA expression levels of all 11 genes were compared by real-time quantitative RT-PCR across nine B and Q field-derived strains of B. tabaci showing strong resistance, moderate resistance or susceptibility to imidacloprid. We found that constitutive over-expression (up to approximately 17-fold) of a single P450 gene, CYP6CM1, was tightly related to imidacloprid resistance in both the B and Q biotypes. Next, we identified three single-nucleotide polymorphic (SNP) markers in the intron region of CYP6CM1 that discriminate between the resistant and susceptible Q-biotype CYP6CM1 alleles (r-Q and s-Q, respectively), and used a heterogeneous strain to test for association between r-Q and resistance. While survivors of a low imidacloprid dose carried both the r-Q and s-Q alleles, approximately 95% of the survivors of a high imidacloprid dose carried only the r-Q allele. Together with previous evidence, the results reported here identify enhanced activity of P450s as the major mechanism of imidacloprid resistance in B. tabaci, and the CYP6CM1 gene as a leading target for DNA-based screening for resistance to imidacloprid and possibly other neonicotinoids in field populations.

Authors+Show Affiliations

Department of Entomology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18510975

Citation

Karunker, Iris, et al. "Over-expression of Cytochrome P450 CYP6CM1 Is Associated With High Resistance to Imidacloprid in the B and Q Biotypes of Bemisia Tabaci (Hemiptera: Aleyrodidae)." Insect Biochemistry and Molecular Biology, vol. 38, no. 6, 2008, pp. 634-44.
Karunker I, Benting J, Lueke B, et al. Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochem Mol Biol. 2008;38(6):634-44.
Karunker, I., Benting, J., Lueke, B., Ponge, T., Nauen, R., Roditakis, E., Vontas, J., Gorman, K., Denholm, I., & Morin, S. (2008). Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochemistry and Molecular Biology, 38(6), 634-44. https://doi.org/10.1016/j.ibmb.2008.03.008
Karunker I, et al. Over-expression of Cytochrome P450 CYP6CM1 Is Associated With High Resistance to Imidacloprid in the B and Q Biotypes of Bemisia Tabaci (Hemiptera: Aleyrodidae). Insect Biochem Mol Biol. 2008;38(6):634-44. PubMed PMID: 18510975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). AU - Karunker,Iris, AU - Benting,Juergen, AU - Lueke,Bettina, AU - Ponge,Tanja, AU - Nauen,Ralf, AU - Roditakis,Emmanouil, AU - Vontas,John, AU - Gorman,Kevin, AU - Denholm,Ian, AU - Morin,Shai, Y1 - 2008/03/29/ PY - 2008/01/03/received PY - 2008/03/20/revised PY - 2008/03/21/accepted PY - 2008/5/31/pubmed PY - 2008/9/5/medline PY - 2008/5/31/entrez SP - 634 EP - 44 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 38 IS - 6 N2 - The two most damaging biotypes of Bemisia tabaci, B and Q, have both evolved strong resistance to the neonicotinoid insecticide imidacloprid. The major mechanism in all samples investigated so far appeared to be enhanced detoxification by cytochrome P450s monooxygenases (P450s). In this study, a polymerase chain reaction (PCR) technology using degenerate primers based on conserved P450 helix I and heme-binding regions was employed to identify P450 cDNA sequences in B. tabaci that might be involved in imidacloprid resistance. Eleven distinct P450 cDNA sequences were isolated and classified as members of the CYP4 or CYP6 families. The mRNA expression levels of all 11 genes were compared by real-time quantitative RT-PCR across nine B and Q field-derived strains of B. tabaci showing strong resistance, moderate resistance or susceptibility to imidacloprid. We found that constitutive over-expression (up to approximately 17-fold) of a single P450 gene, CYP6CM1, was tightly related to imidacloprid resistance in both the B and Q biotypes. Next, we identified three single-nucleotide polymorphic (SNP) markers in the intron region of CYP6CM1 that discriminate between the resistant and susceptible Q-biotype CYP6CM1 alleles (r-Q and s-Q, respectively), and used a heterogeneous strain to test for association between r-Q and resistance. While survivors of a low imidacloprid dose carried both the r-Q and s-Q alleles, approximately 95% of the survivors of a high imidacloprid dose carried only the r-Q allele. Together with previous evidence, the results reported here identify enhanced activity of P450s as the major mechanism of imidacloprid resistance in B. tabaci, and the CYP6CM1 gene as a leading target for DNA-based screening for resistance to imidacloprid and possibly other neonicotinoids in field populations. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/18510975/Over_expression_of_cytochrome_P450_CYP6CM1_is_associated_with_high_resistance_to_imidacloprid_in_the_B_and_Q_biotypes_of_Bemisia_tabaci__Hemiptera:_Aleyrodidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(08)00060-X DB - PRIME DP - Unbound Medicine ER -