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Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats.
C R Biol. 2008 Jun; 331(6):426-32.CR

Abstract

In the present study, we have investigated the influence of sub-acute treatment with cadmium (Cd) on some parameters indicative of oxidative stress and DNA damage in tissues of pregnant female rats. Pregnant female rats (n=6) were injected subcutaneously, daily with a dose of cadmium chloride of 3 mg/kg body weight (b.w.) from day 6 to day 19 of pregnancy, and they were allowed to deliver normally. MDA level and GPx, CAT and SOD activities were used as markers of oxidative stress in liver and kidney. The 8-oxo-dG level was measured by the HPLC-EC system. Cd treatment increased MDA (+116%, p<0.01) in kidney. Moreover, Cd treatment also decreased CuZn-SOD (-11%, p<0.05) and GSH level (-52%, p<0.05) in kidney. Treated rats displayed an increase of the liver metallothionein (MT) level. Induction of MT in liver was probably implicated in the detoxification of Cd. The high level of Cd (3 mg/kg) used in the present study is partially neutralized by MT in liver, whereas the free fraction could be implicated in the oxidative stress and DNA oxidation observed in kidney. Cd treatment failed to alter 8-oxodGuo, indicating the absence of DNA oxidation in liver; by contrast, the same treatment increased the 8-oxodGuo level (+51%, p<0.05) in the kidney of pregnant female rats, indicating an oxidative stress associated with DNA damage only in kidney.

Authors+Show Affiliations

Laboratoire de physiologie intégrée, faculté des sciences de Bizerte, 7021 Jarzouna, Tunisia. bourassihem2002@yahoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18510995

Citation

Chater, Sihem, et al. "Cadmium-induced Oxidative Stress and DNA Damage in Kidney of Pregnant Female Rats." Comptes Rendus Biologies, vol. 331, no. 6, 2008, pp. 426-32.
Chater S, Douki T, Garrel C, et al. Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats. C R Biol. 2008;331(6):426-32.
Chater, S., Douki, T., Garrel, C., Favier, A., Sakly, M., & Abdelmelek, H. (2008). Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats. Comptes Rendus Biologies, 331(6), 426-32. https://doi.org/10.1016/j.crvi.2008.03.009
Chater S, et al. Cadmium-induced Oxidative Stress and DNA Damage in Kidney of Pregnant Female Rats. C R Biol. 2008;331(6):426-32. PubMed PMID: 18510995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats. AU - Chater,Sihem, AU - Douki,Thierry, AU - Garrel,Catherine, AU - Favier,Alain, AU - Sakly,Mohsen, AU - Abdelmelek,Hafedh, Y1 - 2008/04/22/ PY - 2007/08/11/received PY - 2008/03/31/revised PY - 2008/03/31/accepted PY - 2008/5/31/pubmed PY - 2008/8/9/medline PY - 2008/5/31/entrez SP - 426 EP - 32 JF - Comptes rendus biologies JO - C R Biol VL - 331 IS - 6 N2 - In the present study, we have investigated the influence of sub-acute treatment with cadmium (Cd) on some parameters indicative of oxidative stress and DNA damage in tissues of pregnant female rats. Pregnant female rats (n=6) were injected subcutaneously, daily with a dose of cadmium chloride of 3 mg/kg body weight (b.w.) from day 6 to day 19 of pregnancy, and they were allowed to deliver normally. MDA level and GPx, CAT and SOD activities were used as markers of oxidative stress in liver and kidney. The 8-oxo-dG level was measured by the HPLC-EC system. Cd treatment increased MDA (+116%, p<0.01) in kidney. Moreover, Cd treatment also decreased CuZn-SOD (-11%, p<0.05) and GSH level (-52%, p<0.05) in kidney. Treated rats displayed an increase of the liver metallothionein (MT) level. Induction of MT in liver was probably implicated in the detoxification of Cd. The high level of Cd (3 mg/kg) used in the present study is partially neutralized by MT in liver, whereas the free fraction could be implicated in the oxidative stress and DNA oxidation observed in kidney. Cd treatment failed to alter 8-oxodGuo, indicating the absence of DNA oxidation in liver; by contrast, the same treatment increased the 8-oxodGuo level (+51%, p<0.05) in the kidney of pregnant female rats, indicating an oxidative stress associated with DNA damage only in kidney. SN - 1631-0691 UR - https://www.unboundmedicine.com/medline/citation/18510995/Cadmium_induced_oxidative_stress_and_DNA_damage_in_kidney_of_pregnant_female_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1631-0691(08)00084-X DB - PRIME DP - Unbound Medicine ER -