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Interaction between N-terminal loop and beta-scaffold of photoactive yellow protein.
Photochem Photobiol. 2008 Jul-Aug; 84(4):1031-7.PP

Abstract

During the photoreaction cycle of photoactive yellow protein (PYP), a physiologically active intermediate (PYP(M)) is formed as a consequence of global protein conformational change. Previous studies have demonstrated that the photocycle of PYP is regulated by the N-terminal loop region, which is located across the central beta-sheet from the p-coumaric acid chromophore. In this paper, the hydrophobic interaction between N-terminal loop and beta-sheet was studied by characterizing PYP mutants of the hydrophobic residues. The rate constants and structural changes of the photocycle of L15A and L23A possibly participating in such an interaction were more similar to wild-type than F6A, showing that the CH/pi interaction between Phe6 and Lys123 is the most essential as reported previously. To better understand the interactions between N-terminal tail and beta-sheet of PYP, Phe6 and Phe121 were replaced by Cys and linked by a disulfide bond. Since the photocycle kinetics, structural change and thermal stability of F6C/F121C were similar to F6A, the CH/pi interaction between Phe6 and Lys123 is not substitutable. It is likely that the detachment of position 6 from position 123 substantially alters the nature of PYP.

Authors+Show Affiliations

Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18513230

Citation

Harigai, Miki, et al. "Interaction Between N-terminal Loop and Beta-scaffold of Photoactive Yellow Protein." Photochemistry and Photobiology, vol. 84, no. 4, 2008, pp. 1031-7.
Harigai M, Kataoka M, Imamoto Y. Interaction between N-terminal loop and beta-scaffold of photoactive yellow protein. Photochem Photobiol. 2008;84(4):1031-7.
Harigai, M., Kataoka, M., & Imamoto, Y. (2008). Interaction between N-terminal loop and beta-scaffold of photoactive yellow protein. Photochemistry and Photobiology, 84(4), 1031-7. https://doi.org/10.1111/j.1751-1097.2008.00375.x
Harigai M, Kataoka M, Imamoto Y. Interaction Between N-terminal Loop and Beta-scaffold of Photoactive Yellow Protein. Photochem Photobiol. 2008 Jul-Aug;84(4):1031-7. PubMed PMID: 18513230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction between N-terminal loop and beta-scaffold of photoactive yellow protein. AU - Harigai,Miki, AU - Kataoka,Mikio, AU - Imamoto,Yasushi, Y1 - 2008/05/29/ PY - 2008/6/3/pubmed PY - 2008/9/24/medline PY - 2008/6/3/entrez SP - 1031 EP - 7 JF - Photochemistry and photobiology JO - Photochem Photobiol VL - 84 IS - 4 N2 - During the photoreaction cycle of photoactive yellow protein (PYP), a physiologically active intermediate (PYP(M)) is formed as a consequence of global protein conformational change. Previous studies have demonstrated that the photocycle of PYP is regulated by the N-terminal loop region, which is located across the central beta-sheet from the p-coumaric acid chromophore. In this paper, the hydrophobic interaction between N-terminal loop and beta-sheet was studied by characterizing PYP mutants of the hydrophobic residues. The rate constants and structural changes of the photocycle of L15A and L23A possibly participating in such an interaction were more similar to wild-type than F6A, showing that the CH/pi interaction between Phe6 and Lys123 is the most essential as reported previously. To better understand the interactions between N-terminal tail and beta-sheet of PYP, Phe6 and Phe121 were replaced by Cys and linked by a disulfide bond. Since the photocycle kinetics, structural change and thermal stability of F6C/F121C were similar to F6A, the CH/pi interaction between Phe6 and Lys123 is not substitutable. It is likely that the detachment of position 6 from position 123 substantially alters the nature of PYP. SN - 0031-8655 UR - https://www.unboundmedicine.com/medline/citation/18513230/Interaction_between_N_terminal_loop_and_beta_scaffold_of_photoactive_yellow_protein_ L2 - https://doi.org/10.1111/j.1751-1097.2008.00375.x DB - PRIME DP - Unbound Medicine ER -