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Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China.
Acta Vet Scand. 2008 May 31; 50:12.AV

Abstract

BACKGROUND

Hepatitis E virus (HEV) is a zoonotic pathogen of which swine was reported as major reservoirs. HEV has been divided into 4 different genotypes according to phylogenetic analysis. Recent reports showed that genotype 4 HEV is freely transmitted between humans and swine in eastern China, including Shanghai area. This paper investigated the recent infection status of HEV among swine population of Shanghai area in China.

METHODS

480 swine faecal specimens were collected from 23 farms which distribute all over Shanghai from September to November, 2007 and tested for the presence of HEV RNA by the polymerase chain reaction (PCR).

RESULTS

Our results showed that 26.1% (6/23) of the swine farms were positive for HEV RNA and the positive rate of the six farms were ranged from 9.1% to 33.3%. The HEV RNA positive rate for total samples were 5% (24/480). The resulted positive band specific for HEV was sequenced and sequence analysis indicated that all of these isolates belonged to genotype 4 HEV. Phylogenetic analysis showed that the 24 isolates clustered into 4 distinct subgroups, sharing 83.3-89.7% inter-subgroup and 97-99% intra-subgroup identities. More over, isolates in three of the four subgroups closely clustered with previous identified strains, sharing up high to 97% identity with them.

CONCLUSION

These results suggested that there were 4 different subgenotypes of HEV prevalent in Shanghai, and some of them may not be indigenous to Shanghai but introduced from other geographic regions.

Authors+Show Affiliations

Shanghai Key laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai 200240, PR China. y_yan11@sjtu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18513433

Citation

Yan, Yijia, et al. "Prevalence of Four Different Subgenotypes of Genotype 4 Hepatitis E Virus Among Swine in the Shanghai Area of China." Acta Veterinaria Scandinavica, vol. 50, 2008, p. 12.
Yan Y, Zhang W, Shen Q, et al. Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China. Acta Vet Scand. 2008;50:12.
Yan, Y., Zhang, W., Shen, Q., Cui, L., & Hua, X. (2008). Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China. Acta Veterinaria Scandinavica, 50, 12. https://doi.org/10.1186/1751-0147-50-12
Yan Y, et al. Prevalence of Four Different Subgenotypes of Genotype 4 Hepatitis E Virus Among Swine in the Shanghai Area of China. Acta Vet Scand. 2008 May 31;50:12. PubMed PMID: 18513433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of four different subgenotypes of genotype 4 hepatitis E virus among swine in the Shanghai area of China. AU - Yan,Yijia, AU - Zhang,Wen, AU - Shen,Quan, AU - Cui,Li, AU - Hua,Xiuguo, Y1 - 2008/05/31/ PY - 2008/01/19/received PY - 2008/05/31/accepted PY - 2008/6/3/pubmed PY - 2008/8/13/medline PY - 2008/6/3/entrez SP - 12 EP - 12 JF - Acta veterinaria Scandinavica JO - Acta Vet Scand VL - 50 N2 - BACKGROUND: Hepatitis E virus (HEV) is a zoonotic pathogen of which swine was reported as major reservoirs. HEV has been divided into 4 different genotypes according to phylogenetic analysis. Recent reports showed that genotype 4 HEV is freely transmitted between humans and swine in eastern China, including Shanghai area. This paper investigated the recent infection status of HEV among swine population of Shanghai area in China. METHODS: 480 swine faecal specimens were collected from 23 farms which distribute all over Shanghai from September to November, 2007 and tested for the presence of HEV RNA by the polymerase chain reaction (PCR). RESULTS: Our results showed that 26.1% (6/23) of the swine farms were positive for HEV RNA and the positive rate of the six farms were ranged from 9.1% to 33.3%. The HEV RNA positive rate for total samples were 5% (24/480). The resulted positive band specific for HEV was sequenced and sequence analysis indicated that all of these isolates belonged to genotype 4 HEV. Phylogenetic analysis showed that the 24 isolates clustered into 4 distinct subgroups, sharing 83.3-89.7% inter-subgroup and 97-99% intra-subgroup identities. More over, isolates in three of the four subgroups closely clustered with previous identified strains, sharing up high to 97% identity with them. CONCLUSION: These results suggested that there were 4 different subgenotypes of HEV prevalent in Shanghai, and some of them may not be indigenous to Shanghai but introduced from other geographic regions. SN - 1751-0147 UR - https://www.unboundmedicine.com/medline/citation/18513433/Prevalence_of_four_different_subgenotypes_of_genotype_4_hepatitis_E_virus_among_swine_in_the_Shanghai_area_of_China_ L2 - https://actavetscand.biomedcentral.com/articles/10.1186/1751-0147-50-12 DB - PRIME DP - Unbound Medicine ER -