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Conservation of DNA photolyase genes in group II nucleopolyhedroviruses infecting plusiine insects.
Virus Res 2008; 136(1-2):58-64VR

Abstract

DNA photolyase genes (phr) encode photoreactive enzymes, which are involved in the repair of UV-damaged DNA. Cyclobutane pyrimidine dimer (CPD) specific photolyase genes are present in nucleopolyhedroviruses isolated from Chrysodeixis chalcites (ChchNPV) and Trichoplusia ni (TnSNPV), insects belonging to the Plusiinae (Noctuidae). To better understand the occurrence and evolution of these genes in baculoviruses, we investigated their possible conservation in other group II NPVs, which infect plusiine insects. A PCR based strategy using degenerate phr-specific primers was designed to detect and analyze possible photolyase genes. Six additional Plusiinae-infecting NPVs were analyzed and all, except Thysanoplusia oricalcea NPV A28-1, which is a group I NPV, contained one or more phr-like sequences. Phylogenetic analysis revealed that all photolyase genes of the tested Plusiinae-infecting baculoviruses group in a single clade, separated into three subgroups. The phylogeny of the polyhedrin sequences of these viruses confirmed that the analyzed viruses also formed a single clade in group II NPVs. We hypothesize that all plusiine group II NPVs contain one or more photolyase genes and that these have a common ancestor.

Authors+Show Affiliations

Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18513819

Citation

Xu, Fang, et al. "Conservation of DNA Photolyase Genes in Group II Nucleopolyhedroviruses Infecting Plusiine Insects." Virus Research, vol. 136, no. 1-2, 2008, pp. 58-64.
Xu F, Vlak JM, van Oers MM. Conservation of DNA photolyase genes in group II nucleopolyhedroviruses infecting plusiine insects. Virus Res. 2008;136(1-2):58-64.
Xu, F., Vlak, J. M., & van Oers, M. M. (2008). Conservation of DNA photolyase genes in group II nucleopolyhedroviruses infecting plusiine insects. Virus Research, 136(1-2), pp. 58-64. doi:10.1016/j.virusres.2008.04.017.
Xu F, Vlak JM, van Oers MM. Conservation of DNA Photolyase Genes in Group II Nucleopolyhedroviruses Infecting Plusiine Insects. Virus Res. 2008;136(1-2):58-64. PubMed PMID: 18513819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conservation of DNA photolyase genes in group II nucleopolyhedroviruses infecting plusiine insects. AU - Xu,Fang, AU - Vlak,Just M, AU - van Oers,Monique M, Y1 - 2008/05/29/ PY - 2008/01/21/received PY - 2008/04/18/revised PY - 2008/04/18/accepted PY - 2008/6/3/pubmed PY - 2008/9/24/medline PY - 2008/6/3/entrez SP - 58 EP - 64 JF - Virus research JO - Virus Res. VL - 136 IS - 1-2 N2 - DNA photolyase genes (phr) encode photoreactive enzymes, which are involved in the repair of UV-damaged DNA. Cyclobutane pyrimidine dimer (CPD) specific photolyase genes are present in nucleopolyhedroviruses isolated from Chrysodeixis chalcites (ChchNPV) and Trichoplusia ni (TnSNPV), insects belonging to the Plusiinae (Noctuidae). To better understand the occurrence and evolution of these genes in baculoviruses, we investigated their possible conservation in other group II NPVs, which infect plusiine insects. A PCR based strategy using degenerate phr-specific primers was designed to detect and analyze possible photolyase genes. Six additional Plusiinae-infecting NPVs were analyzed and all, except Thysanoplusia oricalcea NPV A28-1, which is a group I NPV, contained one or more phr-like sequences. Phylogenetic analysis revealed that all photolyase genes of the tested Plusiinae-infecting baculoviruses group in a single clade, separated into three subgroups. The phylogeny of the polyhedrin sequences of these viruses confirmed that the analyzed viruses also formed a single clade in group II NPVs. We hypothesize that all plusiine group II NPVs contain one or more photolyase genes and that these have a common ancestor. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/18513819/Conservation_of_DNA_photolyase_genes_in_group_II_nucleopolyhedroviruses_infecting_plusiine_insects_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(08)00165-2 DB - PRIME DP - Unbound Medicine ER -