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Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C.
Vet Microbiol 2008; 131(3-4):406-13VM

Abstract

The objectives of this study were to identify haemagglutinin (HA) epitopes of Avibacterium paragallinarum serovar C that are capable of eliciting haemagglutination inhibition (HI) antibody, and to investigate their immunogenic role. Three conformational epitopes were detected on HA by blocking ELISA and immuno-dot blot analysis using a panel of five monoclonal antibodies (MAbs) with HI activity, designated 8C1C, 4G8B, 24E4D, 11E11B, and 10D1A. The minimum DNA regions coding these three epitopes were 3195, 2862, and 807bp in size, and mapped within a gene with 6117bp. Nine DNA fragments of various lengths were prepared, and their recombinant proteins were generated in E. coli. One recombinant protein, designated HPC5.5, was recognized by MAb 8C1C, and had strong ability to adsorb HI antibody to Av. paragallinarum serovar C. Other recombinant proteins designated HPC5.1, HPC4.8, and HPC2.5 did not react with MAb 8C1C and only slightly adsorbed HI antibody. All chickens immunized once with HPC5.5 did not show any typical clinical signs such as nasal discharge or facial edema against challenge inoculation with Av. paragallinarum serovar C. However, HPC5.1, which was recognized by four MAbs (not including MAb 8C1C), showed only partial protective immunity in five of eight immunized chickens. The results suggest that the HA epitope recognized by MAb 8C1C is the major epitope responsible for eliciting HI antibody, and HPC5.5 is a practical candidate protein to develop a new vaccine against avian infectious coryza caused by Av. paragallinarum serovar C.

Authors+Show Affiliations

Kyoto Biken Laboratories, Inc., 24-16 Makishima-cho, Uji-shi, Kyoto 611-0041, Japan. kenkai@kyotobiken.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18514440

Citation

Noro, Taichi, et al. "Identification and Characterization of Haemagglutinin Epitopes of Avibacterium Paragallinarum Serovar C." Veterinary Microbiology, vol. 131, no. 3-4, 2008, pp. 406-13.
Noro T, Oishi E, Kaneshige T, et al. Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C. Vet Microbiol. 2008;131(3-4):406-13.
Noro, T., Oishi, E., Kaneshige, T., Yaguchi, K., Amimoto, K., & Shimizu, M. (2008). Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C. Veterinary Microbiology, 131(3-4), pp. 406-13. doi:10.1016/j.vetmic.2008.04.010.
Noro T, et al. Identification and Characterization of Haemagglutinin Epitopes of Avibacterium Paragallinarum Serovar C. Vet Microbiol. 2008 Oct 15;131(3-4):406-13. PubMed PMID: 18514440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C. AU - Noro,Taichi, AU - Oishi,Eiji, AU - Kaneshige,Takahiro, AU - Yaguchi,Kazuhiko, AU - Amimoto,Katsuhiko, AU - Shimizu,Mitsugu, Y1 - 2008/06/02/ PY - 2008/01/25/received PY - 2008/03/24/revised PY - 2008/04/10/accepted PY - 2008/6/3/pubmed PY - 2008/12/17/medline PY - 2008/6/3/entrez SP - 406 EP - 13 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 131 IS - 3-4 N2 - The objectives of this study were to identify haemagglutinin (HA) epitopes of Avibacterium paragallinarum serovar C that are capable of eliciting haemagglutination inhibition (HI) antibody, and to investigate their immunogenic role. Three conformational epitopes were detected on HA by blocking ELISA and immuno-dot blot analysis using a panel of five monoclonal antibodies (MAbs) with HI activity, designated 8C1C, 4G8B, 24E4D, 11E11B, and 10D1A. The minimum DNA regions coding these three epitopes were 3195, 2862, and 807bp in size, and mapped within a gene with 6117bp. Nine DNA fragments of various lengths were prepared, and their recombinant proteins were generated in E. coli. One recombinant protein, designated HPC5.5, was recognized by MAb 8C1C, and had strong ability to adsorb HI antibody to Av. paragallinarum serovar C. Other recombinant proteins designated HPC5.1, HPC4.8, and HPC2.5 did not react with MAb 8C1C and only slightly adsorbed HI antibody. All chickens immunized once with HPC5.5 did not show any typical clinical signs such as nasal discharge or facial edema against challenge inoculation with Av. paragallinarum serovar C. However, HPC5.1, which was recognized by four MAbs (not including MAb 8C1C), showed only partial protective immunity in five of eight immunized chickens. The results suggest that the HA epitope recognized by MAb 8C1C is the major epitope responsible for eliciting HI antibody, and HPC5.5 is a practical candidate protein to develop a new vaccine against avian infectious coryza caused by Av. paragallinarum serovar C. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/18514440/Identification_and_characterization_of_haemagglutinin_epitopes_of_Avibacterium_paragallinarum_serovar_C_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(08)00145-4 DB - PRIME DP - Unbound Medicine ER -