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A role of brassinosteroids in early fruit development in cucumber.
J Exp Bot. 2008; 59(9):2299-308.JE

Abstract

Brassinosteroids (BRs) are essential for many biological processes in plants, however, little is known about their roles in early fruit development. To address this, BR levels were manipulated through the application of exogenous BRs (24-epibrassinolide, EBR) or a BR biosynthesis inhibitor (brassinazole, Brz) and their effects on early fruit development, cell division, and expression of cyclin and cyclin-dependent kinases (CDKs) genes were examined in two cucumber cultivars that differ in parthenocarpic capacity. The application of EBR induced parthenocarpic growth accompanied by active cell division in Jinchun No. 4, a cultivar without parthenocarpic capacity, whereas Brz treatment inhibited fruit set and, subsequently, fruit growth in Jinchun No. 2, a cultivar with natural parthenocarpic capacity, and this inhibitory effect could be rescued by the application of EBR. RT-PCR analysis showed both pollination and EBR induced expression of cell cycle-related genes (CycA, CycB, CycD3;1, CycD3;2, and CDKB) after anthesis. cDNA sequences for CsCycD3;1 and CsCycD3;2 were isolated through PCR amplification. Both CsCycD3;1 and CsCycD3;2 transcripts were up-regulated by EBR treatment and pollination but strongly repressed by Brz treatment. Meanwhile, BR6ox1 and SMT transcripts, two genes involved in BR synthesis, exhibited feedback regulation. These results strongly suggest that BRs play an important role during early fruit development in cucumber.

Authors+Show Affiliations

Department of Horticulture, Huajiachi Campus, Zhejiang University, Kaixuan Road 268, Hangzhou, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18515830

Citation

Fu, Feng Qing, et al. "A Role of Brassinosteroids in Early Fruit Development in Cucumber." Journal of Experimental Botany, vol. 59, no. 9, 2008, pp. 2299-308.
Fu FQ, Mao WH, Shi K, et al. A role of brassinosteroids in early fruit development in cucumber. J Exp Bot. 2008;59(9):2299-308.
Fu, F. Q., Mao, W. H., Shi, K., Zhou, Y. H., Asami, T., & Yu, J. Q. (2008). A role of brassinosteroids in early fruit development in cucumber. Journal of Experimental Botany, 59(9), 2299-308. https://doi.org/10.1093/jxb/ern093
Fu FQ, et al. A Role of Brassinosteroids in Early Fruit Development in Cucumber. J Exp Bot. 2008;59(9):2299-308. PubMed PMID: 18515830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A role of brassinosteroids in early fruit development in cucumber. AU - Fu,Feng Qing, AU - Mao,Wei Hua, AU - Shi,Kai, AU - Zhou,Yan Hong, AU - Asami,Tadao, AU - Yu,Jing Quan, Y1 - 2008/05/31/ PY - 2008/6/3/pubmed PY - 2008/8/7/medline PY - 2008/6/3/entrez SP - 2299 EP - 308 JF - Journal of experimental botany JO - J Exp Bot VL - 59 IS - 9 N2 - Brassinosteroids (BRs) are essential for many biological processes in plants, however, little is known about their roles in early fruit development. To address this, BR levels were manipulated through the application of exogenous BRs (24-epibrassinolide, EBR) or a BR biosynthesis inhibitor (brassinazole, Brz) and their effects on early fruit development, cell division, and expression of cyclin and cyclin-dependent kinases (CDKs) genes were examined in two cucumber cultivars that differ in parthenocarpic capacity. The application of EBR induced parthenocarpic growth accompanied by active cell division in Jinchun No. 4, a cultivar without parthenocarpic capacity, whereas Brz treatment inhibited fruit set and, subsequently, fruit growth in Jinchun No. 2, a cultivar with natural parthenocarpic capacity, and this inhibitory effect could be rescued by the application of EBR. RT-PCR analysis showed both pollination and EBR induced expression of cell cycle-related genes (CycA, CycB, CycD3;1, CycD3;2, and CDKB) after anthesis. cDNA sequences for CsCycD3;1 and CsCycD3;2 were isolated through PCR amplification. Both CsCycD3;1 and CsCycD3;2 transcripts were up-regulated by EBR treatment and pollination but strongly repressed by Brz treatment. Meanwhile, BR6ox1 and SMT transcripts, two genes involved in BR synthesis, exhibited feedback regulation. These results strongly suggest that BRs play an important role during early fruit development in cucumber. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/18515830/A_role_of_brassinosteroids_in_early_fruit_development_in_cucumber_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ern093 DB - PRIME DP - Unbound Medicine ER -