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Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro.
Nephrology (Carlton). 2008 Oct; 13(7):607-15.N

Abstract

BACKGROUND

Decorin (DCN) is a small leucine-rich proteoglycan that plays an important role in the regulation of intercellular contact, cell migration and proliferation. DCN suppresses cell growth and induces apoptosis in various tumour cells. The aim of this study was to investigate whether overexpression of DCN could induce apoptosis and cell growth arrest in mesangial cells (MsCs) in vitro.

METHODS

PcDNA3.1A-DCN plasmid was transfected into cultured rat MsCs, and positive clones stably expressing DCN (MsC/DCN) were selected. SiRNA was used for blocking DCN expression in MsC/DCN. Apoptosis and cell growth of MsCs were assayed by flow cytometry. Hoechst staining was used for observing apoptotic cells. Expressions of active Caspase-3, epidermal growth factor receptor (EGFR), P21 and transforming growth factor-beta (TGF-beta1) were analyzed using Western blot.

RESULTS

Overexpression of DCN in MsCs induced apoptosis and arrested cells in the G(0)/G(1) phase. The protein level of active Caspase-3 was significantly elevated in MsC/DCN (P < 0.01). DCN transfection induced downregulation of EGFR and up-expression of P21. In addition, the expression of TGF-beta1 was significantly inhibited. DCN-siRNA transfection remarkably blocked the expression of DCN and reversed the downregulatory effects of DCN on MsC's proliferation.

CONCLUSION

Overexpression of DCN could inhibit MsCs proliferation by inducing apoptosis and cell growth arrest in vitro and it also downregulates expression of TGF-beta1. These results suggest novel strategies for regulating the proliferation of MsC in glomerular diseases.

Authors+Show Affiliations

Department of Pathology and Key Laboratory of Molecular Medicine, Ministry of Education of China, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18518935

Citation

Wu, Huijuan, et al. "Overexpression of Decorin Induces Apoptosis and Cell Growth Arrest in Cultured Rat Mesangial Cells in Vitro." Nephrology (Carlton, Vic.), vol. 13, no. 7, 2008, pp. 607-15.
Wu H, Wang S, Xue A, et al. Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro. Nephrology (Carlton). 2008;13(7):607-15.
Wu, H., Wang, S., Xue, A., Liu, Y., Liu, Y., Wang, H., Chen, Q., Guo, M., & Zhang, Z. (2008). Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro. Nephrology (Carlton, Vic.), 13(7), 607-15. https://doi.org/10.1111/j.1440-1797.2008.00961.x
Wu H, et al. Overexpression of Decorin Induces Apoptosis and Cell Growth Arrest in Cultured Rat Mesangial Cells in Vitro. Nephrology (Carlton). 2008;13(7):607-15. PubMed PMID: 18518935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of decorin induces apoptosis and cell growth arrest in cultured rat mesangial cells in vitro. AU - Wu,Huijuan, AU - Wang,Suxia, AU - Xue,Aimin, AU - Liu,Yuan, AU - Liu,Ye, AU - Wang,Huijun, AU - Chen,Qi, AU - Guo,Muyi, AU - Zhang,Zhigang, Y1 - 2008/06/01/ PY - 2008/6/4/pubmed PY - 2009/3/12/medline PY - 2008/6/4/entrez SP - 607 EP - 15 JF - Nephrology (Carlton, Vic.) JO - Nephrology (Carlton) VL - 13 IS - 7 N2 - BACKGROUND: Decorin (DCN) is a small leucine-rich proteoglycan that plays an important role in the regulation of intercellular contact, cell migration and proliferation. DCN suppresses cell growth and induces apoptosis in various tumour cells. The aim of this study was to investigate whether overexpression of DCN could induce apoptosis and cell growth arrest in mesangial cells (MsCs) in vitro. METHODS: PcDNA3.1A-DCN plasmid was transfected into cultured rat MsCs, and positive clones stably expressing DCN (MsC/DCN) were selected. SiRNA was used for blocking DCN expression in MsC/DCN. Apoptosis and cell growth of MsCs were assayed by flow cytometry. Hoechst staining was used for observing apoptotic cells. Expressions of active Caspase-3, epidermal growth factor receptor (EGFR), P21 and transforming growth factor-beta (TGF-beta1) were analyzed using Western blot. RESULTS: Overexpression of DCN in MsCs induced apoptosis and arrested cells in the G(0)/G(1) phase. The protein level of active Caspase-3 was significantly elevated in MsC/DCN (P < 0.01). DCN transfection induced downregulation of EGFR and up-expression of P21. In addition, the expression of TGF-beta1 was significantly inhibited. DCN-siRNA transfection remarkably blocked the expression of DCN and reversed the downregulatory effects of DCN on MsC's proliferation. CONCLUSION: Overexpression of DCN could inhibit MsCs proliferation by inducing apoptosis and cell growth arrest in vitro and it also downregulates expression of TGF-beta1. These results suggest novel strategies for regulating the proliferation of MsC in glomerular diseases. SN - 1440-1797 UR - https://www.unboundmedicine.com/medline/citation/18518935/Overexpression_of_decorin_induces_apoptosis_and_cell_growth_arrest_in_cultured_rat_mesangial_cells_in_vitro_ L2 - https://doi.org/10.1111/j.1440-1797.2008.00961.x DB - PRIME DP - Unbound Medicine ER -