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Huang-lian-jie-du-tang, a traditional Chinese medicine prescription, induces cell-cycle arrest and apoptosis in human liver cancer cells in vitro and in vivo.
J Gastroenterol Hepatol. 2008 Jul; 23(7 Pt 2):e290-9.JG

Abstract

BACKGROUND AND AIM

Huang-lian-jie-du-tang (HLJDT; Japanese name, oren-gedoku-to) is a traditional Chinese medicine prescription known to possess anti-inflammatory activity. Our study reports here for the first time the anticancer effect of HLJDT in two human liver cancer cell lines, Hep G2 and PLC/PRF/5.

METHODS

Inhibition of cell proliferation by HLJDT was measured by sodium 3'-(1-(phenylamino-carbonyl)-3,4-tetrazolium)-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT) assay. Clonogenic assay was used to elucidate the possible differences in long-term effects of HLJDT on human liver cancer cells. Cell cycle distribution was determined by flow cytometry. Apoptosis was detected using electrophoresis and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick endlabeling (TUNEL) assay. Protein expressions were determined by immunoblot assay. The activity of nuclear factor-kappa B (NF-kappaB) was determined by Trans-AM ELISA kit. In vivo tumor activity was assessed by xenograft study.

RESULTS

HLJDT significantly increased the expression of inactivated phospho-Cdc2 and phospho-Cdc25C, and decreased the levels of cyclin A, cyclin B1, Cdc2, and Cdc25C, thereby contributing to cell-cycle arrest. HLJDT increased the expression of Bax and Bak, but decreased the level of Bcl-2 and Bcl-XL, and subsequently triggered the mitochondrial apoptotic pathway. In addition, HLJDT also inhibited cell-survival signaling by enhancing the amount of IkappaBalpha in the cytoplasm, reducing the level and activity of NF-kappaB in the nucleus, and subsequently attenuating the expression of Bcl-XL in Hep G2 and PLC/PRF/5 cells(.) The inhibitory effect mediated by HLJDT on cell growth was also demonstrated in a nude mouse model, in which the liver cancer cells induced tumor xenograft shrank considerably following treatment with HLJDT.

CONCLUSIONS

Taken together, these results suggest a potential anticancer effect of HLJDT against human liver cancer cells.

Authors+Show Affiliations

Cell Biology Laboratory, Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan. s_udhema@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18522681

Citation

Hsu, Ya-Ling, et al. "Huang-lian-jie-du-tang, a Traditional Chinese Medicine Prescription, Induces Cell-cycle Arrest and Apoptosis in Human Liver Cancer Cells in Vitro and in Vivo." Journal of Gastroenterology and Hepatology, vol. 23, no. 7 Pt 2, 2008, pp. e290-9.
Hsu YL, Kuo PL, Tzeng TF, et al. Huang-lian-jie-du-tang, a traditional Chinese medicine prescription, induces cell-cycle arrest and apoptosis in human liver cancer cells in vitro and in vivo. J Gastroenterol Hepatol. 2008;23(7 Pt 2):e290-9.
Hsu, Y. L., Kuo, P. L., Tzeng, T. F., Sung, S. C., Yen, M. H., Lin, L. T., & Lin, C. C. (2008). Huang-lian-jie-du-tang, a traditional Chinese medicine prescription, induces cell-cycle arrest and apoptosis in human liver cancer cells in vitro and in vivo. Journal of Gastroenterology and Hepatology, 23(7 Pt 2), e290-9. https://doi.org/10.1111/j.1440-1746.2008.05390.x
Hsu YL, et al. Huang-lian-jie-du-tang, a Traditional Chinese Medicine Prescription, Induces Cell-cycle Arrest and Apoptosis in Human Liver Cancer Cells in Vitro and in Vivo. J Gastroenterol Hepatol. 2008;23(7 Pt 2):e290-9. PubMed PMID: 18522681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Huang-lian-jie-du-tang, a traditional Chinese medicine prescription, induces cell-cycle arrest and apoptosis in human liver cancer cells in vitro and in vivo. AU - Hsu,Ya-Ling, AU - Kuo,Po-Lin, AU - Tzeng,Tz-Fei, AU - Sung,Shu-Chiao, AU - Yen,Ming-Hong, AU - Lin,Liang-Tzung, AU - Lin,Chun-Ching, Y1 - 2008/06/03/ PY - 2008/6/5/pubmed PY - 2008/10/31/medline PY - 2008/6/5/entrez SP - e290 EP - 9 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 23 IS - 7 Pt 2 N2 - BACKGROUND AND AIM: Huang-lian-jie-du-tang (HLJDT; Japanese name, oren-gedoku-to) is a traditional Chinese medicine prescription known to possess anti-inflammatory activity. Our study reports here for the first time the anticancer effect of HLJDT in two human liver cancer cell lines, Hep G2 and PLC/PRF/5. METHODS: Inhibition of cell proliferation by HLJDT was measured by sodium 3'-(1-(phenylamino-carbonyl)-3,4-tetrazolium)-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT) assay. Clonogenic assay was used to elucidate the possible differences in long-term effects of HLJDT on human liver cancer cells. Cell cycle distribution was determined by flow cytometry. Apoptosis was detected using electrophoresis and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick endlabeling (TUNEL) assay. Protein expressions were determined by immunoblot assay. The activity of nuclear factor-kappa B (NF-kappaB) was determined by Trans-AM ELISA kit. In vivo tumor activity was assessed by xenograft study. RESULTS: HLJDT significantly increased the expression of inactivated phospho-Cdc2 and phospho-Cdc25C, and decreased the levels of cyclin A, cyclin B1, Cdc2, and Cdc25C, thereby contributing to cell-cycle arrest. HLJDT increased the expression of Bax and Bak, but decreased the level of Bcl-2 and Bcl-XL, and subsequently triggered the mitochondrial apoptotic pathway. In addition, HLJDT also inhibited cell-survival signaling by enhancing the amount of IkappaBalpha in the cytoplasm, reducing the level and activity of NF-kappaB in the nucleus, and subsequently attenuating the expression of Bcl-XL in Hep G2 and PLC/PRF/5 cells(.) The inhibitory effect mediated by HLJDT on cell growth was also demonstrated in a nude mouse model, in which the liver cancer cells induced tumor xenograft shrank considerably following treatment with HLJDT. CONCLUSIONS: Taken together, these results suggest a potential anticancer effect of HLJDT against human liver cancer cells. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/18522681/Huang_lian_jie_du_tang_a_traditional_Chinese_medicine_prescription_induces_cell_cycle_arrest_and_apoptosis_in_human_liver_cancer_cells_in_vitro_and_in_vivo_ L2 - https://doi.org/10.1111/j.1440-1746.2008.05390.x DB - PRIME DP - Unbound Medicine ER -