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Clinical impact of plasma TGF-beta1 and circulating TGF-beta1 mRNA in diagnosis of hepatocellular carcinoma.
Hepatobiliary Pancreat Dis Int. 2008 Jun; 7(3):288-95.HP

Abstract

BACKGROUND

Transforming growth factor-beta (TGF-beta) plays an important role in the regulation of cell growth and differentiation, angiogenesis, extracellular matrix formation, immunosuppression and cancer development. In this study, we investigated the levels of TGF-beta1 and TGF-beta1 mRNA expression, their relationship with HBV replication, and their diagnostic value for hepatocellular carcinoma (HCC).

METHODS

Total RNAs were extracted from HCC samples and matched non-tumor tissues, and from peripheral blood mononuclear cells in HCC patients. TGF-beta1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-beta1 expression was assessed by immunohistochemistry. The clinical characteristics were analyzed between TGF-beta1 and HBV replication. The diagnostic value of circulating TGF-beta1 and TGF-beta1 mRNA levels were investigated in HCC patients.

RESULTS

The incidence of hepatic TGF-beta1 expression was 83.3% in HCC samples, 43.3% in the surrounding tissues, 94.7% in the HBV DNA-positive group, and 63.6% in the HBV DNA-negative group. Liver TGF-beta1 expression was associated with the degree of HCC differentiation and the status of HBV replication, but not with the size or number of tumors. Circulating TGF-beta1 level and incidence of TGF-beta1 mRNA were significantly higher in the HCC group than in any group of patients with benign liver disease, with a higher sensitivity of 89.5% and a specificity of 94.0% for HCC diagnosis when circulating TGF-beta1 levels were >1.2 microg/L. No significant correlation was found between TGF-beta1 expression and AFP level or tumor size. Combining TGF-beta1 level and serum AFP raised the detection rate to 97.4%.

CONCLUSIONS

Abnormal expression of hepatic TGF-beta1 is associated with the degree of HCC differentiation and HBV replication. Both circulating TGF-beta1 and TGF-beta1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC.

Authors+Show Affiliations

Department of Diagnostics, Affiliated Hospital, Nantong University, Nantong 226001, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18522884

Citation

Dong, Zhi-Zhen, et al. "Clinical Impact of Plasma TGF-beta1 and Circulating TGF-beta1 mRNA in Diagnosis of Hepatocellular Carcinoma." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 7, no. 3, 2008, pp. 288-95.
Dong ZZ, Yao DF, Yao M, et al. Clinical impact of plasma TGF-beta1 and circulating TGF-beta1 mRNA in diagnosis of hepatocellular carcinoma. Hepatobiliary Pancreat Dis Int. 2008;7(3):288-95.
Dong, Z. Z., Yao, D. F., Yao, M., Qiu, L. W., Zong, L., Wu, W., Wu, X. H., Yao, D. B., & Meng, X. Y. (2008). Clinical impact of plasma TGF-beta1 and circulating TGF-beta1 mRNA in diagnosis of hepatocellular carcinoma. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 7(3), 288-95.
Dong ZZ, et al. Clinical Impact of Plasma TGF-beta1 and Circulating TGF-beta1 mRNA in Diagnosis of Hepatocellular Carcinoma. Hepatobiliary Pancreat Dis Int. 2008;7(3):288-95. PubMed PMID: 18522884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical impact of plasma TGF-beta1 and circulating TGF-beta1 mRNA in diagnosis of hepatocellular carcinoma. AU - Dong,Zhi-Zhen, AU - Yao,Deng-Fu, AU - Yao,Min, AU - Qiu,Li-Wei, AU - Zong,Lei, AU - Wu,Wei, AU - Wu,Xin-Hua, AU - Yao,Deng-Bing, AU - Meng,Xian-Yong, PY - 2008/6/5/pubmed PY - 2008/11/5/medline PY - 2008/6/5/entrez SP - 288 EP - 95 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - Hepatobiliary Pancreat Dis Int VL - 7 IS - 3 N2 - BACKGROUND: Transforming growth factor-beta (TGF-beta) plays an important role in the regulation of cell growth and differentiation, angiogenesis, extracellular matrix formation, immunosuppression and cancer development. In this study, we investigated the levels of TGF-beta1 and TGF-beta1 mRNA expression, their relationship with HBV replication, and their diagnostic value for hepatocellular carcinoma (HCC). METHODS: Total RNAs were extracted from HCC samples and matched non-tumor tissues, and from peripheral blood mononuclear cells in HCC patients. TGF-beta1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-beta1 expression was assessed by immunohistochemistry. The clinical characteristics were analyzed between TGF-beta1 and HBV replication. The diagnostic value of circulating TGF-beta1 and TGF-beta1 mRNA levels were investigated in HCC patients. RESULTS: The incidence of hepatic TGF-beta1 expression was 83.3% in HCC samples, 43.3% in the surrounding tissues, 94.7% in the HBV DNA-positive group, and 63.6% in the HBV DNA-negative group. Liver TGF-beta1 expression was associated with the degree of HCC differentiation and the status of HBV replication, but not with the size or number of tumors. Circulating TGF-beta1 level and incidence of TGF-beta1 mRNA were significantly higher in the HCC group than in any group of patients with benign liver disease, with a higher sensitivity of 89.5% and a specificity of 94.0% for HCC diagnosis when circulating TGF-beta1 levels were >1.2 microg/L. No significant correlation was found between TGF-beta1 expression and AFP level or tumor size. Combining TGF-beta1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS: Abnormal expression of hepatic TGF-beta1 is associated with the degree of HCC differentiation and HBV replication. Both circulating TGF-beta1 and TGF-beta1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/18522884/Clinical_impact_of_plasma_TGF_beta1_and_circulating_TGF_beta1_mRNA_in_diagnosis_of_hepatocellular_carcinoma_ L2 - https://medlineplus.gov/livercancer.html DB - PRIME DP - Unbound Medicine ER -