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Effect of tumor necrosis factor-alpha in rats with hepatic ischemia-reperfusion injury.
Hepatobiliary Pancreat Dis Int. 2008 Jun; 7(3):296-9.HP

Abstract

BACKGROUND

With the development of hepatic surgery, especially liver transplantation, the pathophysiological processes of hepatic ischemia-reperfusion (I/R) injury have gained special attention. Controlling I/R injury has become one of the most important factors for successful liver transplantation. This study aimed to investigate the effects of tumor necrosis factor-alpha (TNF-alpha) in rats with hepatic I/R injury and promote the recognition of I/R injury in the liver.

METHODS

Thirty-two Sprague-Dawley rats were randomly divided into 2 groups. Rats in the sham-operated (SO) group served as controls. Rats in the hepatic ischemia-reperfusion (I/R) group underwent reperfusion after 30 minutes of liver ischemia. Rats were sacrificed at 1, 6 and 12 hours. The expression of TNF-alpha mRNA in the liver was measured by RT-PCR. Histological changes in the liver were assessed. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were measured.

RESULTS

The expression of TNF-alpha mRNA in the SO group was decreased compared with that in the I/R group (P<0.05). TNF-alpha mRNA expression progressively increased in the I/R group. The serum levels of ALT and AST in the I/R group were higher than those in the SO group (P<0.01). The histological changes were in accord with hepatic I/R injury.

CONCLUSION

ALT and AST in serum are closely related to hepatic I/R injury and inflammatory reaction. TNF-alpha production in the liver triggers hepatic I/R injury through a cascade.

Authors+Show Affiliations

Ma M
Department of Geriatric Surgery, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. Mamao2007@163.com
Ma ZH
No affiliation info available

MeSH

Alanine TransaminaseAnimalsAspartate AminotransferasesDisease Models, AnimalDisease ProgressionGene ExpressionLiverLiver DiseasesRNA, MessengerRatsRats, Sprague-DawleyReperfusion InjuryReverse Transcriptase Polymerase Chain ReactionSeverity of Illness IndexTumor Necrosis Factor-alpha

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18522885

Citation

Ma, Mao, and Zhen-Hua Ma. "Effect of Tumor Necrosis Factor-alpha in Rats With Hepatic Ischemia-reperfusion Injury." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 7, no. 3, 2008, pp. 296-9.
Ma M, Ma ZH. Effect of tumor necrosis factor-alpha in rats with hepatic ischemia-reperfusion injury. Hepatobiliary Pancreat Dis Int. 2008;7(3):296-9.
Ma, M., & Ma, Z. H. (2008). Effect of tumor necrosis factor-alpha in rats with hepatic ischemia-reperfusion injury. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 7(3), 296-9.
Ma M, Ma ZH. Effect of Tumor Necrosis Factor-alpha in Rats With Hepatic Ischemia-reperfusion Injury. Hepatobiliary Pancreat Dis Int. 2008;7(3):296-9. PubMed PMID: 18522885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of tumor necrosis factor-alpha in rats with hepatic ischemia-reperfusion injury. AU - Ma,Mao, AU - Ma,Zhen-Hua, PY - 2008/6/5/pubmed PY - 2008/11/5/medline PY - 2008/6/5/entrez SP - 296 EP - 9 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - Hepatobiliary Pancreat Dis Int VL - 7 IS - 3 N2 - BACKGROUND: With the development of hepatic surgery, especially liver transplantation, the pathophysiological processes of hepatic ischemia-reperfusion (I/R) injury have gained special attention. Controlling I/R injury has become one of the most important factors for successful liver transplantation. This study aimed to investigate the effects of tumor necrosis factor-alpha (TNF-alpha) in rats with hepatic I/R injury and promote the recognition of I/R injury in the liver. METHODS: Thirty-two Sprague-Dawley rats were randomly divided into 2 groups. Rats in the sham-operated (SO) group served as controls. Rats in the hepatic ischemia-reperfusion (I/R) group underwent reperfusion after 30 minutes of liver ischemia. Rats were sacrificed at 1, 6 and 12 hours. The expression of TNF-alpha mRNA in the liver was measured by RT-PCR. Histological changes in the liver were assessed. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were measured. RESULTS: The expression of TNF-alpha mRNA in the SO group was decreased compared with that in the I/R group (P<0.05). TNF-alpha mRNA expression progressively increased in the I/R group. The serum levels of ALT and AST in the I/R group were higher than those in the SO group (P<0.01). The histological changes were in accord with hepatic I/R injury. CONCLUSION: ALT and AST in serum are closely related to hepatic I/R injury and inflammatory reaction. TNF-alpha production in the liver triggers hepatic I/R injury through a cascade. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/18522885/Effect_of_tumor_necrosis_factor_alpha_in_rats_with_hepatic_ischemia_reperfusion_injury_ L2 - https://medlineplus.gov/liverdiseases.html DB - PRIME DP - Unbound Medicine ER -