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Significance of isolated positive IgM serologic results by enzyme immunoassay for coccidioidomycosis.
Mycopathologia. 2008 Aug; 166(2):77-82.M

Abstract

Serologic testing is important for diagnosis of coccidioidomycosis. Many methods are available for diagnostic testing. Enzyme immunoassay (EIA) can be performed quickly and locally but has the potential for false-positive results in patients manifesting a positive EIA for immunoglobulin M (IgM) antibodies and a negative EIA for immunoglobulin G (IgG). We retrospectively reviewed the charts of 405 patients with coccidioidal serologic testing performed between 1999 and 2003. Of 706 EIAs, 37 (5%) produced test results for 28 patients that showed isolated IgM positivity. Among these 28 patients, 24 (86%) had positive serologic findings by other methods (complement fixation or immunodiffusion or both), and 7 (25%) had positive microbiologic or histopathologic findings. All 4 (14%) patients without other positive serologic results had diagnostic tests with positive microbiologic or histopathologic results. No false-positive IgM assays were observed. We conclude that the false-positive rate of the EIA IgM is low, and that an isolated positive EIA IgM should prompt further follow-up and diagnostic testing.

Authors+Show Affiliations

Division of Infectious Diseases, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ, 85259, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18523863

Citation

Blair, Janis E., and Jeremiah T. Currier. "Significance of Isolated Positive IgM Serologic Results By Enzyme Immunoassay for Coccidioidomycosis." Mycopathologia, vol. 166, no. 2, 2008, pp. 77-82.
Blair JE, Currier JT. Significance of isolated positive IgM serologic results by enzyme immunoassay for coccidioidomycosis. Mycopathologia. 2008;166(2):77-82.
Blair, J. E., & Currier, J. T. (2008). Significance of isolated positive IgM serologic results by enzyme immunoassay for coccidioidomycosis. Mycopathologia, 166(2), 77-82. https://doi.org/10.1007/s11046-008-9129-9
Blair JE, Currier JT. Significance of Isolated Positive IgM Serologic Results By Enzyme Immunoassay for Coccidioidomycosis. Mycopathologia. 2008;166(2):77-82. PubMed PMID: 18523863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Significance of isolated positive IgM serologic results by enzyme immunoassay for coccidioidomycosis. AU - Blair,Janis E, AU - Currier,Jeremiah T, Y1 - 2008/06/04/ PY - 2007/11/07/received PY - 2008/04/22/accepted PY - 2008/6/5/pubmed PY - 2008/10/1/medline PY - 2008/6/5/entrez SP - 77 EP - 82 JF - Mycopathologia JO - Mycopathologia VL - 166 IS - 2 N2 - Serologic testing is important for diagnosis of coccidioidomycosis. Many methods are available for diagnostic testing. Enzyme immunoassay (EIA) can be performed quickly and locally but has the potential for false-positive results in patients manifesting a positive EIA for immunoglobulin M (IgM) antibodies and a negative EIA for immunoglobulin G (IgG). We retrospectively reviewed the charts of 405 patients with coccidioidal serologic testing performed between 1999 and 2003. Of 706 EIAs, 37 (5%) produced test results for 28 patients that showed isolated IgM positivity. Among these 28 patients, 24 (86%) had positive serologic findings by other methods (complement fixation or immunodiffusion or both), and 7 (25%) had positive microbiologic or histopathologic findings. All 4 (14%) patients without other positive serologic results had diagnostic tests with positive microbiologic or histopathologic results. No false-positive IgM assays were observed. We conclude that the false-positive rate of the EIA IgM is low, and that an isolated positive EIA IgM should prompt further follow-up and diagnostic testing. SN - 0301-486X UR - https://www.unboundmedicine.com/medline/citation/18523863/Significance_of_isolated_positive_IgM_serologic_results_by_enzyme_immunoassay_for_coccidioidomycosis_ L2 - https://doi.org/10.1007/s11046-008-9129-9 DB - PRIME DP - Unbound Medicine ER -