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TRPM8 activation suppresses cellular viability in human melanoma.
Am J Physiol Cell Physiol. 2008 Aug; 295(2):C296-301.AJ

Abstract

The transient receptor potential melastatin subfamily (TRPM), which is a mammalian homologue of cell death-regulated genes in Caenorhabditis elegans and Drosophila, has potential roles in the process of the cell cycle and regulation of Ca(2+) signaling. Among this subfamily, TRPM8 (also known as Trp-p8) is a Ca(2+)-permeable channel that was originally identified as a prostate-specific gene upregulated in tumors. Here we showed that the TRPM8 channel was expressed in human melanoma G-361 cells, and activation of the channel produced sustainable Ca(2+) influx. The application of menthol, an agonist for TRPM8 channel, elevated cytosolic Ca(2+) concentration in a concentration-dependent manner with an EC(50) value of 286 microM in melanoma cells. Menthol-induced responses were significantly abolished by the removal of external Ca(2+). Moreover, inward currents at a holding potential of -60 mV in melanoma cells were markedly potentiated by the addition of 300 microM menthol. The most striking finding was that the viability of melanoma cells was dose-dependently depressed in the presence of menthol. These results reveal that a functional TRPM8 protein is expressed in human melanoma cells to involve the mechanism underlying tumor progression via the Ca(2+) handling pathway, providing us with a novel target of drug development for malignant melanoma.

Authors+Show Affiliations

Dept. of Molecular Morphology, Graduate School of Medical Sciences, Nagoya City Univ., 1 Kawasumi Mizuhocho Mizuhoku, Nagoya 467-8601, Japan. yamamura@med.nagoya-cu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18524940

Citation

Yamamura, Hisao, et al. "TRPM8 Activation Suppresses Cellular Viability in Human Melanoma." American Journal of Physiology. Cell Physiology, vol. 295, no. 2, 2008, pp. C296-301.
Yamamura H, Ugawa S, Ueda T, et al. TRPM8 activation suppresses cellular viability in human melanoma. Am J Physiol Cell Physiol. 2008;295(2):C296-301.
Yamamura, H., Ugawa, S., Ueda, T., Morita, A., & Shimada, S. (2008). TRPM8 activation suppresses cellular viability in human melanoma. American Journal of Physiology. Cell Physiology, 295(2), C296-301. https://doi.org/10.1152/ajpcell.00499.2007
Yamamura H, et al. TRPM8 Activation Suppresses Cellular Viability in Human Melanoma. Am J Physiol Cell Physiol. 2008;295(2):C296-301. PubMed PMID: 18524940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPM8 activation suppresses cellular viability in human melanoma. AU - Yamamura,Hisao, AU - Ugawa,Shinya, AU - Ueda,Takashi, AU - Morita,Akimichi, AU - Shimada,Shoichi, Y1 - 2008/06/04/ PY - 2008/6/6/pubmed PY - 2008/11/14/medline PY - 2008/6/6/entrez SP - C296 EP - 301 JF - American journal of physiology. Cell physiology JO - Am J Physiol Cell Physiol VL - 295 IS - 2 N2 - The transient receptor potential melastatin subfamily (TRPM), which is a mammalian homologue of cell death-regulated genes in Caenorhabditis elegans and Drosophila, has potential roles in the process of the cell cycle and regulation of Ca(2+) signaling. Among this subfamily, TRPM8 (also known as Trp-p8) is a Ca(2+)-permeable channel that was originally identified as a prostate-specific gene upregulated in tumors. Here we showed that the TRPM8 channel was expressed in human melanoma G-361 cells, and activation of the channel produced sustainable Ca(2+) influx. The application of menthol, an agonist for TRPM8 channel, elevated cytosolic Ca(2+) concentration in a concentration-dependent manner with an EC(50) value of 286 microM in melanoma cells. Menthol-induced responses were significantly abolished by the removal of external Ca(2+). Moreover, inward currents at a holding potential of -60 mV in melanoma cells were markedly potentiated by the addition of 300 microM menthol. The most striking finding was that the viability of melanoma cells was dose-dependently depressed in the presence of menthol. These results reveal that a functional TRPM8 protein is expressed in human melanoma cells to involve the mechanism underlying tumor progression via the Ca(2+) handling pathway, providing us with a novel target of drug development for malignant melanoma. SN - 0363-6143 UR - https://www.unboundmedicine.com/medline/citation/18524940/TRPM8_activation_suppresses_cellular_viability_in_human_melanoma_ L2 - https://journals.physiology.org/doi/10.1152/ajpcell.00499.2007?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -