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Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice.
Eur Surg Res 2008; 41(2):231-7ES

Abstract

BACKGROUND

Propolis is a natural product collected by honey bees from various plant sources. We aimed to determine the possible effects of propolis on oxidative stress and hepatocyte apoptosis in experimental obstructive jaundice.

METHODS

Thirty rats were divided into three groups: group I, sham-operated; group II, ligation and division of the common bile duct (BDL); group III, BDL followed by oral supplementation of propolis in a daily dose of 100 mg/kg. Liver samples were examined under the light microscope and transmission electron microscope. Hepatocyte apoptosis was quantitated using the transferase-mediated uridine nick end labeling (TUNEL) assay. Plasma and liver malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) activities were measured.

RESULTS

The plasma and liver levels of MDA were significantly lower in the propolis group than in the BDL group (p < 0.05 and 0.014, respectively). Although liver GSH-Px activities were significantly higher in the propolis group than in the BDL group (p < 0.001), there was no significant difference between the plasma GSH-Px activities of these groups (p > 0.05). In the propolis group, the enlargement of hepatocytes, dilatation of canaliculi and the edema regressed. The regenerating and normal hepatocytes were demonstrated. In the TUNEL assay, propolis administration reduced hepatocyte apoptosis.

CONCLUSION

Propolis showed a significant hepatoprotective effect in this experimental obstructive jaundice model.

Authors+Show Affiliations

4th General Surgery Department, Ankara Training and Research Hospital, Ankara, Turkey. kemalkismet@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18525208

Citation

Kismet, K, et al. "Effect of Propolis On Oxidative Stress and Histomorphology of Liver Tissue in Experimental Obstructive Jaundice." European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes, vol. 41, no. 2, 2008, pp. 231-7.
Kismet K, Sabuncuoglu MZ, Kilicoglu SS, et al. Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice. Eur Surg Res. 2008;41(2):231-7.
Kismet, K., Sabuncuoglu, M. Z., Kilicoglu, S. S., Kilicoglu, B., Devrim, E., Erel, S., ... Akkus, M. A. (2008). Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice. European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes, 41(2), pp. 231-7. doi:10.1159/000136479.
Kismet K, et al. Effect of Propolis On Oxidative Stress and Histomorphology of Liver Tissue in Experimental Obstructive Jaundice. Eur Surg Res. 2008;41(2):231-7. PubMed PMID: 18525208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice. AU - Kismet,K, AU - Sabuncuoglu,M Z, AU - Kilicoglu,S S, AU - Kilicoglu,B, AU - Devrim,E, AU - Erel,S, AU - Sunay,A E, AU - Erdemli,E, AU - Durak,I, AU - Akkus,M A, Y1 - 2008/06/05/ PY - 2007/10/17/received PY - 2008/02/27/accepted PY - 2008/6/6/pubmed PY - 2008/11/14/medline PY - 2008/6/6/entrez SP - 231 EP - 7 JF - European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes JO - Eur Surg Res VL - 41 IS - 2 N2 - BACKGROUND: Propolis is a natural product collected by honey bees from various plant sources. We aimed to determine the possible effects of propolis on oxidative stress and hepatocyte apoptosis in experimental obstructive jaundice. METHODS: Thirty rats were divided into three groups: group I, sham-operated; group II, ligation and division of the common bile duct (BDL); group III, BDL followed by oral supplementation of propolis in a daily dose of 100 mg/kg. Liver samples were examined under the light microscope and transmission electron microscope. Hepatocyte apoptosis was quantitated using the transferase-mediated uridine nick end labeling (TUNEL) assay. Plasma and liver malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) activities were measured. RESULTS: The plasma and liver levels of MDA were significantly lower in the propolis group than in the BDL group (p < 0.05 and 0.014, respectively). Although liver GSH-Px activities were significantly higher in the propolis group than in the BDL group (p < 0.001), there was no significant difference between the plasma GSH-Px activities of these groups (p > 0.05). In the propolis group, the enlargement of hepatocytes, dilatation of canaliculi and the edema regressed. The regenerating and normal hepatocytes were demonstrated. In the TUNEL assay, propolis administration reduced hepatocyte apoptosis. CONCLUSION: Propolis showed a significant hepatoprotective effect in this experimental obstructive jaundice model. SN - 1421-9921 UR - https://www.unboundmedicine.com/medline/citation/18525208/Effect_of_propolis_on_oxidative_stress_and_histomorphology_of_liver_tissue_in_experimental_obstructive_jaundice_ L2 - https://www.karger.com?DOI=10.1159/000136479 DB - PRIME DP - Unbound Medicine ER -