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Mechanisms of copper toxicity in Saccharomyces cerevisiae determined by microarray analysis.
Environ Toxicol. 2008 Oct; 23(5):599-606.ET

Abstract

The effect of the heavy metal copper on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. The gene expression profile of baker's yeast Saccharomyces cerevisiae grown in a medium containing a sublethal concentration of cupric sulfate was compared with that of yeast grown in a normal medium. Among approximately 6000 yeast ORFs, 143 ORFs were induced more than twofold to resist copper toxicity after exposure to copper. Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold. Some genes related to sulfur metabolism and oxidative stress response were also up-regulated. This DNA microarray analysis identified several molecular targets of copper toxicity.

Authors+Show Affiliations

Hokkaido Food Processing Research Center, 589-4 Bunkyodai Midorimachi, Ebetsu, Hokkaido 0690836, Japan. duke@foodhokkaido.gr.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18528910

Citation

Yasokawa, Daisuke, et al. "Mechanisms of Copper Toxicity in Saccharomyces Cerevisiae Determined By Microarray Analysis." Environmental Toxicology, vol. 23, no. 5, 2008, pp. 599-606.
Yasokawa D, Murata S, Kitagawa E, et al. Mechanisms of copper toxicity in Saccharomyces cerevisiae determined by microarray analysis. Environ Toxicol. 2008;23(5):599-606.
Yasokawa, D., Murata, S., Kitagawa, E., Iwahashi, Y., Nakagawa, R., Hashido, T., & Iwahashi, H. (2008). Mechanisms of copper toxicity in Saccharomyces cerevisiae determined by microarray analysis. Environmental Toxicology, 23(5), 599-606. https://doi.org/10.1002/tox.20406
Yasokawa D, et al. Mechanisms of Copper Toxicity in Saccharomyces Cerevisiae Determined By Microarray Analysis. Environ Toxicol. 2008;23(5):599-606. PubMed PMID: 18528910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of copper toxicity in Saccharomyces cerevisiae determined by microarray analysis. AU - Yasokawa,Daisuke, AU - Murata,Satomi, AU - Kitagawa,Emiko, AU - Iwahashi,Yumiko, AU - Nakagawa,Ryoji, AU - Hashido,Tazusa, AU - Iwahashi,Hitoshi, PY - 2008/6/6/pubmed PY - 2008/12/17/medline PY - 2008/6/6/entrez SP - 599 EP - 606 JF - Environmental toxicology JO - Environ Toxicol VL - 23 IS - 5 N2 - The effect of the heavy metal copper on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. The gene expression profile of baker's yeast Saccharomyces cerevisiae grown in a medium containing a sublethal concentration of cupric sulfate was compared with that of yeast grown in a normal medium. Among approximately 6000 yeast ORFs, 143 ORFs were induced more than twofold to resist copper toxicity after exposure to copper. Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold. Some genes related to sulfur metabolism and oxidative stress response were also up-regulated. This DNA microarray analysis identified several molecular targets of copper toxicity. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/18528910/Mechanisms_of_copper_toxicity_in_Saccharomyces_cerevisiae_determined_by_microarray_analysis_ DB - PRIME DP - Unbound Medicine ER -