Tags

Type your tag names separated by a space and hit enter

Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts isolated from Bacopa monniera (L.) Wettst.
J Ethnopharmacol. 2008 Jul 23; 118(2):305-11.JE

Abstract

AIM OF THE STUDY

Bacopa monniera Linn is described in the Ayurvedic Materia Medica, as a therapeutically useful herb for the treatment of inflammation. In the current study, we investigated the anti-inflammatory activity of methanolic extract of Bacopa monniera (BME). For some experiments EtOAc and bacoside fractions were prepared from BME. The effect of these extracts in modulating key mediators of inflammation was evaluated.

MATERIALS AND METHODS

Carrageenan-induced rat paw edema, rat mononuclear cells and human whole blood assay were employed as in vivo and in vitro models.

RESULTS

In carrageenan-induced rat paw edema, BME brought about 82% edema inhibition at a dose of 100mg/kg i.p. when compared to indomethacin (INDO) (3mg/kg) that showed 70% edema inhibition. BME also significantly inhibited 5-lipoxygenase (5-LOX), 15-LOX and cyclooxygenase-2 (COX-2) activities in rat monocytes in vivo. Among the fractions tested in vitro, EtOAc fraction possessed significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity with IC(50) value of 30 microg/ml compared to butylated hydroxyl toluene (IC(50) = 13 microg/ml). This fraction also exerted significant hydroxyl radical scavenging activity with IC(50) value of 25 microg/ml in comparison with quercetin (IC(50) = 5 microg/ml). Inhibitory effects of EtOAc and bacoside fractions on LOX and COX activities in Ca-A23187 stimulated rat mononuclear cells were also assessed. 5-LOX IC(50) values were 25 microg/ml for EtOAc, 68 microg/ml for bacosides and 2 microg/ml for nordihydroguaiaretic acid (NDGA) where as COX-2 IC(50) values were 1.32 microg/ml for EtOAc, 1.19 microg/ml for bacoside fraction and 0.23 microg/ml for indomethacin. EtOAc and bacoside fractions also brought about significant decrease in TNF-alpha release ex vivo.

CONCLUSION

Bacopa monniera possesses anti-inflammatory activity through inhibition of COX and LOX and downregulation of TNF-alpha.

Links

Publisher Full Text

Authors+Show Affiliations

Viji V
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala 695 581, India.
Helen A
No affiliation info available

MeSH

AnimalsAnti-Inflammatory AgentsBacopaCyclooxygenase 2Disease Models, AnimalDose-Response Relationship, DrugDown-RegulationEdemaHumansIndiaIndomethacinInflammationInhibitory Concentration 50LipoxygenaseMaleMedicine, AyurvedicMonocytesPlant ExtractsRatsRats, WistarTumor Necrosis Factor-alpha

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18534796

Citation

Viji, V, and A Helen. "Inhibition of Lipoxygenases and Cyclooxygenase-2 Enzymes By Extracts Isolated From Bacopa Monniera (L.) Wettst." Journal of Ethnopharmacology, vol. 118, no. 2, 2008, pp. 305-11.
Viji V, Helen A. Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts isolated from Bacopa monniera (L.) Wettst. J Ethnopharmacol. 2008;118(2):305-11.
Viji, V., & Helen, A. (2008). Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts isolated from Bacopa monniera (L.) Wettst. Journal of Ethnopharmacology, 118(2), 305-11. https://doi.org/10.1016/j.jep.2008.04.017
Viji V, Helen A. Inhibition of Lipoxygenases and Cyclooxygenase-2 Enzymes By Extracts Isolated From Bacopa Monniera (L.) Wettst. J Ethnopharmacol. 2008 Jul 23;118(2):305-11. PubMed PMID: 18534796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts isolated from Bacopa monniera (L.) Wettst. AU - Viji,V, AU - Helen,A, Y1 - 2008/04/24/ PY - 2007/10/16/received PY - 2008/04/12/revised PY - 2008/04/20/accepted PY - 2008/6/7/pubmed PY - 2008/12/17/medline PY - 2008/6/7/entrez SP - 305 EP - 11 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 118 IS - 2 N2 - AIM OF THE STUDY: Bacopa monniera Linn is described in the Ayurvedic Materia Medica, as a therapeutically useful herb for the treatment of inflammation. In the current study, we investigated the anti-inflammatory activity of methanolic extract of Bacopa monniera (BME). For some experiments EtOAc and bacoside fractions were prepared from BME. The effect of these extracts in modulating key mediators of inflammation was evaluated. MATERIALS AND METHODS: Carrageenan-induced rat paw edema, rat mononuclear cells and human whole blood assay were employed as in vivo and in vitro models. RESULTS: In carrageenan-induced rat paw edema, BME brought about 82% edema inhibition at a dose of 100mg/kg i.p. when compared to indomethacin (INDO) (3mg/kg) that showed 70% edema inhibition. BME also significantly inhibited 5-lipoxygenase (5-LOX), 15-LOX and cyclooxygenase-2 (COX-2) activities in rat monocytes in vivo. Among the fractions tested in vitro, EtOAc fraction possessed significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity with IC(50) value of 30 microg/ml compared to butylated hydroxyl toluene (IC(50) = 13 microg/ml). This fraction also exerted significant hydroxyl radical scavenging activity with IC(50) value of 25 microg/ml in comparison with quercetin (IC(50) = 5 microg/ml). Inhibitory effects of EtOAc and bacoside fractions on LOX and COX activities in Ca-A23187 stimulated rat mononuclear cells were also assessed. 5-LOX IC(50) values were 25 microg/ml for EtOAc, 68 microg/ml for bacosides and 2 microg/ml for nordihydroguaiaretic acid (NDGA) where as COX-2 IC(50) values were 1.32 microg/ml for EtOAc, 1.19 microg/ml for bacoside fraction and 0.23 microg/ml for indomethacin. EtOAc and bacoside fractions also brought about significant decrease in TNF-alpha release ex vivo. CONCLUSION: Bacopa monniera possesses anti-inflammatory activity through inhibition of COX and LOX and downregulation of TNF-alpha. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18534796/Inhibition_of_lipoxygenases_and_cyclooxygenase_2_enzymes_by_extracts_isolated_from_Bacopa_monniera__L___Wettst_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00220-1 DB - PRIME DP - Unbound Medicine ER -