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Lipid profiling of developing Jatropha curcas L. seeds using (1)H NMR spectroscopy.
Bioresour Technol. 2008 Dec; 99(18):9032-5.BT

Abstract

Seed development in Jatropha curcas L. was studied with respect to phenology, oil content, lipid profile and concentration of sterols. Seeds were collected at various stages of development starting from one week after fertilization and in an interval of five days thereafter till maturity. These were classified as stage I to stage VII. Moisture content of the seeds ranged from 8.8 to 90.3%; the lowest in mature seeds in stage VII and highest in stage I. The seed area increased as the seed grew from stage I to stage VI (0.2-10.2mm(2) per seed), however, the seed area shrunk at stage VII. Increase in seed area corresponded to increase in fresh weight of the seeds. (1)H NMR spectroscopy of hexane extracts made at different stages of seed development revealed the presence of free fatty acids (FFA), methyl esters of fatty acids (FAME) and triglycerol esters (TAG), along with small quantity of sterols. The young seeds synthesized predominantly polar lipids. Lipid synthesis was noticed nearly three weeks after fertilization. From the fourth week the seeds actively synthesized TAG. Stage III is a turning point in seed development since at this stage, the concentration of sterols decreased to negligible, there was very little FAME formation, accumulation of TAG increased substantially, and there was a sudden decrease in FFA concentration. The findings can be helpful in understanding the biosynthesis and in efforts to improve biosynthesis of TAG and reduce FFA content in the mature seeds.

Authors+Show Affiliations

Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post-graduate Institute of Medical Sciences, Lucknow 226 014, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18534845

Citation

Annarao, Sanjay, et al. "Lipid Profiling of Developing Jatropha Curcas L. Seeds Using (1)H NMR Spectroscopy." Bioresource Technology, vol. 99, no. 18, 2008, pp. 9032-5.
Annarao S, Sidhu OP, Roy R, et al. Lipid profiling of developing Jatropha curcas L. seeds using (1)H NMR spectroscopy. Bioresour Technol. 2008;99(18):9032-5.
Annarao, S., Sidhu, O. P., Roy, R., Tuli, R., & Khetrapal, C. L. (2008). Lipid profiling of developing Jatropha curcas L. seeds using (1)H NMR spectroscopy. Bioresource Technology, 99(18), 9032-5. https://doi.org/10.1016/j.biortech.2008.04.023
Annarao S, et al. Lipid Profiling of Developing Jatropha Curcas L. Seeds Using (1)H NMR Spectroscopy. Bioresour Technol. 2008;99(18):9032-5. PubMed PMID: 18534845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid profiling of developing Jatropha curcas L. seeds using (1)H NMR spectroscopy. AU - Annarao,Sanjay, AU - Sidhu,O P, AU - Roy,Raja, AU - Tuli,Rakesh, AU - Khetrapal,C L, Y1 - 2008/06/04/ PY - 2008/01/02/received PY - 2008/04/05/revised PY - 2008/04/08/accepted PY - 2008/6/7/pubmed PY - 2008/12/17/medline PY - 2008/6/7/entrez SP - 9032 EP - 5 JF - Bioresource technology JO - Bioresour Technol VL - 99 IS - 18 N2 - Seed development in Jatropha curcas L. was studied with respect to phenology, oil content, lipid profile and concentration of sterols. Seeds were collected at various stages of development starting from one week after fertilization and in an interval of five days thereafter till maturity. These were classified as stage I to stage VII. Moisture content of the seeds ranged from 8.8 to 90.3%; the lowest in mature seeds in stage VII and highest in stage I. The seed area increased as the seed grew from stage I to stage VI (0.2-10.2mm(2) per seed), however, the seed area shrunk at stage VII. Increase in seed area corresponded to increase in fresh weight of the seeds. (1)H NMR spectroscopy of hexane extracts made at different stages of seed development revealed the presence of free fatty acids (FFA), methyl esters of fatty acids (FAME) and triglycerol esters (TAG), along with small quantity of sterols. The young seeds synthesized predominantly polar lipids. Lipid synthesis was noticed nearly three weeks after fertilization. From the fourth week the seeds actively synthesized TAG. Stage III is a turning point in seed development since at this stage, the concentration of sterols decreased to negligible, there was very little FAME formation, accumulation of TAG increased substantially, and there was a sudden decrease in FFA concentration. The findings can be helpful in understanding the biosynthesis and in efforts to improve biosynthesis of TAG and reduce FFA content in the mature seeds. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/18534845/Lipid_profiling_of_developing_Jatropha_curcas_L__seeds_using__1_H_NMR_spectroscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(08)00345-3 DB - PRIME DP - Unbound Medicine ER -