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Genotoxic damage in Solea senegalensis exposed to sediments from the Sado Estuary (Portugal): effects of metallic and organic contaminants.
Mutat Res. 2008 Jun 30; 654(1):29-37.MR

Abstract

Juvenile Solea senegalensis (Senegalese sole) were exposed to freshly collected sediments from three sites of the Sado Estuary (West-Portuguese coast) in 28-day laboratory assays in order to assess the ecological risk from sediment contaminants, by measuring two genotoxicity biomarkers in peripheral blood: the percentage of Erythrocyte Nuclear Abnormalities (ENA) by use of an adaptation of the micronucleus test, and the percentage of DNA strand-breakage (DNA-SB) with the Comet assay. Sediments were surveyed for metallic (Cr, Ni, Cu, Zn, As, Cd and Pb) and organic (PAHs (polycyclic aromatic hydrocarbons), PCBs (polychlorinated biphenyls) and DDTs (dichloro-diphenyl-trichloroethane)) contaminants. Sediments from site A (farthest from hotspots of contamination) were found to be the least contaminated and weaker inducers of genotoxic damage, whereas sediments from sites B (urban influence) and C (affected by industrial effluents and agricultural runoffs) were responsible for a very significant increase in both ENA and DNA-SB, site B being most contaminated with metals and site C mainly with organic pollutants, especially PAHs and PCBs . Analysis of genotoxic effects showed a strong correlation between the concentrations of PAHs and PCBs and both biomarkers at sampling times T(14) and T(28), while the amounts of Cu, As, Cd and Pb were less strongly correlated, and at T(28) only, with ENA and DNA-SB. These results show that organic contaminants in sediment are stronger and faster acting genotoxic stressors. The results also suggest that metals may have an inhibitory effect on genotoxicity when interacting with organic contaminants, at least during early exposure. ENA and DNA-SB do not show a linear relationship, but a strong correlation exists between the overall increase in genotoxicity caused by exposure to sediment, confirming that they are different, and possibly non-linked effects that respond similarly to exposure. Although the Comet assay showed enhanced sensitivity, the two analyses are complementary and suitable for the biomonitoring of sediment contaminants in a benthic species like S. senegalensis.

Authors+Show Affiliations

IMAR - Instituto do Mar, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal. pmcosta@fct.unl.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18534898

Citation

Costa, Pedro M., et al. "Genotoxic Damage in Solea Senegalensis Exposed to Sediments From the Sado Estuary (Portugal): Effects of Metallic and Organic Contaminants." Mutation Research, vol. 654, no. 1, 2008, pp. 29-37.
Costa PM, Lobo J, Caeiro S, et al. Genotoxic damage in Solea senegalensis exposed to sediments from the Sado Estuary (Portugal): effects of metallic and organic contaminants. Mutat Res. 2008;654(1):29-37.
Costa, P. M., Lobo, J., Caeiro, S., Martins, M., Ferreira, A. M., Caetano, M., Vale, C., Delvalls, T. A., & Costa, M. H. (2008). Genotoxic damage in Solea senegalensis exposed to sediments from the Sado Estuary (Portugal): effects of metallic and organic contaminants. Mutation Research, 654(1), 29-37. https://doi.org/10.1016/j.mrgentox.2008.04.007
Costa PM, et al. Genotoxic Damage in Solea Senegalensis Exposed to Sediments From the Sado Estuary (Portugal): Effects of Metallic and Organic Contaminants. Mutat Res. 2008 Jun 30;654(1):29-37. PubMed PMID: 18534898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic damage in Solea senegalensis exposed to sediments from the Sado Estuary (Portugal): effects of metallic and organic contaminants. AU - Costa,Pedro M, AU - Lobo,Jorge, AU - Caeiro,Sandra, AU - Martins,Marta, AU - Ferreira,Ana M, AU - Caetano,Miguel, AU - Vale,Carlos, AU - Delvalls,T Angel, AU - Costa,Maria H, Y1 - 2008/04/30/ PY - 2007/11/21/received PY - 2008/03/14/revised PY - 2008/04/20/accepted PY - 2008/6/7/pubmed PY - 2008/9/16/medline PY - 2008/6/7/entrez SP - 29 EP - 37 JF - Mutation research JO - Mutat Res VL - 654 IS - 1 N2 - Juvenile Solea senegalensis (Senegalese sole) were exposed to freshly collected sediments from three sites of the Sado Estuary (West-Portuguese coast) in 28-day laboratory assays in order to assess the ecological risk from sediment contaminants, by measuring two genotoxicity biomarkers in peripheral blood: the percentage of Erythrocyte Nuclear Abnormalities (ENA) by use of an adaptation of the micronucleus test, and the percentage of DNA strand-breakage (DNA-SB) with the Comet assay. Sediments were surveyed for metallic (Cr, Ni, Cu, Zn, As, Cd and Pb) and organic (PAHs (polycyclic aromatic hydrocarbons), PCBs (polychlorinated biphenyls) and DDTs (dichloro-diphenyl-trichloroethane)) contaminants. Sediments from site A (farthest from hotspots of contamination) were found to be the least contaminated and weaker inducers of genotoxic damage, whereas sediments from sites B (urban influence) and C (affected by industrial effluents and agricultural runoffs) were responsible for a very significant increase in both ENA and DNA-SB, site B being most contaminated with metals and site C mainly with organic pollutants, especially PAHs and PCBs . Analysis of genotoxic effects showed a strong correlation between the concentrations of PAHs and PCBs and both biomarkers at sampling times T(14) and T(28), while the amounts of Cu, As, Cd and Pb were less strongly correlated, and at T(28) only, with ENA and DNA-SB. These results show that organic contaminants in sediment are stronger and faster acting genotoxic stressors. The results also suggest that metals may have an inhibitory effect on genotoxicity when interacting with organic contaminants, at least during early exposure. ENA and DNA-SB do not show a linear relationship, but a strong correlation exists between the overall increase in genotoxicity caused by exposure to sediment, confirming that they are different, and possibly non-linked effects that respond similarly to exposure. Although the Comet assay showed enhanced sensitivity, the two analyses are complementary and suitable for the biomonitoring of sediment contaminants in a benthic species like S. senegalensis. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/18534898/Genotoxic_damage_in_Solea_senegalensis_exposed_to_sediments_from_the_Sado_Estuary__Portugal_:_effects_of_metallic_and_organic_contaminants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(08)00129-0 DB - PRIME DP - Unbound Medicine ER -