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Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods.
Food Microbiol 2008; 25(5):668-75FM

Abstract

Enterococci can be used in the food industry as starter or probiotic cultures. However, enterococci are also implicated in severe multi-resistant nosocomial infections. In this study, the prevalence of enterococci in selected Brazilian foodstuffs (raw and pasteurized milk, meat products, cheeses and vegetables) was evaluated. Phenotypic and PCR protocols were used for species identification. Tests for production of gelatinase, haemolysin, bacteriocin and bile salt hydrolysis were done with all enterococci isolates, whereas molecular determination of virulence markers (genes esp, gel, ace, as, efaA, hyl and cylA) and antibiotic resistance was checked only for Enterococcus faecium and Enterococcus faecalis isolates. The antibiotic-resistant isolates were assayed for biofilm formation and adhesion to mammalian cells. From the 120 food samples analyzed, 52.5% were positive for enterococci, meat and cheese being the most contaminated. E. faecium was the predominant species, followed by E. faecalis, E. casseliflavus and Enterococcus gallinarum. Phenotypic tests indicated that 67.7% of isolates hydrolyzed bile salts, 15.2% produced bacteriocin, 12.0% were beta-hemolytic and 18.2% produced gelatinase. Antibiotic resistance (gentamicin, tetracycline and erythromycin) and genes encoding for virulence traits were more frequent in E. faecalis than in E. faecium. Three E. faecium isolates were resistant to vancomycin. Among antibiotic-resistant isolates, 72.4% of E. faecalis were able to form biofilm and 13.8% to adhere to Caco-2 cells. Antibiotic-resistant E. faecalis and E. faecium isolates were grouped by RAPD-PCR and a scattered distribution was noted, indicating that resistance was not related to a particular clone. The spread of virulence/resistance traits in isolates of the two species and different RAPD-types suggest the pathogenic potential of both species. By contrast, the recovery of bacteriocinogenic E. faecium isolates with no virulence traits suggests their potential for biotechnological applications. In conclusion, our results showed that enterococci from Brazilian foods present important dualist aspects for food safety.

Authors+Show Affiliations

Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av Prof Lineu Prestes 580, Bloco 13B, 05508-900 São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18541165

Citation

Gomes, Bruna C., et al. "Prevalence and Characterization of Enterococcus Spp. Isolated From Brazilian Foods." Food Microbiology, vol. 25, no. 5, 2008, pp. 668-75.
Gomes BC, Esteves CT, Palazzo IC, et al. Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. Food Microbiol. 2008;25(5):668-75.
Gomes, B. C., Esteves, C. T., Palazzo, I. C., Darini, A. L., Felis, G. E., Sechi, L. A., ... De Martinis, E. C. (2008). Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. Food Microbiology, 25(5), pp. 668-75. doi:10.1016/j.fm.2008.03.008.
Gomes BC, et al. Prevalence and Characterization of Enterococcus Spp. Isolated From Brazilian Foods. Food Microbiol. 2008;25(5):668-75. PubMed PMID: 18541165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. AU - Gomes,Bruna C, AU - Esteves,Carolina T, AU - Palazzo,Izabel C V, AU - Darini,Ana Lúcia C, AU - Felis,G E, AU - Sechi,Leonardo A, AU - Franco,Bernadette D G M, AU - De Martinis,Elaine C P, Y1 - 2008/04/07/ PY - 2007/11/26/received PY - 2008/03/19/revised PY - 2008/03/30/accepted PY - 2008/6/11/pubmed PY - 2008/8/19/medline PY - 2008/6/11/entrez SP - 668 EP - 75 JF - Food microbiology JO - Food Microbiol. VL - 25 IS - 5 N2 - Enterococci can be used in the food industry as starter or probiotic cultures. However, enterococci are also implicated in severe multi-resistant nosocomial infections. In this study, the prevalence of enterococci in selected Brazilian foodstuffs (raw and pasteurized milk, meat products, cheeses and vegetables) was evaluated. Phenotypic and PCR protocols were used for species identification. Tests for production of gelatinase, haemolysin, bacteriocin and bile salt hydrolysis were done with all enterococci isolates, whereas molecular determination of virulence markers (genes esp, gel, ace, as, efaA, hyl and cylA) and antibiotic resistance was checked only for Enterococcus faecium and Enterococcus faecalis isolates. The antibiotic-resistant isolates were assayed for biofilm formation and adhesion to mammalian cells. From the 120 food samples analyzed, 52.5% were positive for enterococci, meat and cheese being the most contaminated. E. faecium was the predominant species, followed by E. faecalis, E. casseliflavus and Enterococcus gallinarum. Phenotypic tests indicated that 67.7% of isolates hydrolyzed bile salts, 15.2% produced bacteriocin, 12.0% were beta-hemolytic and 18.2% produced gelatinase. Antibiotic resistance (gentamicin, tetracycline and erythromycin) and genes encoding for virulence traits were more frequent in E. faecalis than in E. faecium. Three E. faecium isolates were resistant to vancomycin. Among antibiotic-resistant isolates, 72.4% of E. faecalis were able to form biofilm and 13.8% to adhere to Caco-2 cells. Antibiotic-resistant E. faecalis and E. faecium isolates were grouped by RAPD-PCR and a scattered distribution was noted, indicating that resistance was not related to a particular clone. The spread of virulence/resistance traits in isolates of the two species and different RAPD-types suggest the pathogenic potential of both species. By contrast, the recovery of bacteriocinogenic E. faecium isolates with no virulence traits suggests their potential for biotechnological applications. In conclusion, our results showed that enterococci from Brazilian foods present important dualist aspects for food safety. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/18541165/Prevalence_and_characterization_of_Enterococcus_spp__isolated_from_Brazilian_foods_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(08)00055-5 DB - PRIME DP - Unbound Medicine ER -