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An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters.
Psychopharmacology (Berl). 2008 Oct; 200(3):333-46.P

Abstract

RATIONALE

An endocannabinoid signaling system has not been identified in hamsters.

OBJECTIVE

We examined the existence of an endocannabinoid signaling system in Syrian hamsters using neuroanatomical, biochemical, and behavioral pharmacological approaches.

MATERIALS AND METHODS

The distribution of cannabinoid receptors was mapped, and membrane fatty-acid amide hydrolase (FAAH) activity and levels of fatty-acid amides were measured in hamster brain. The impact of cannabinoid CB1 receptor blockade and inhibition of FAAH was evaluated in the elevated plus maze, rota-rod test, and models of unconditioned and conditioned social defeat.

RESULTS

A characteristic heterogeneous distribution of cannabinoid receptors was detected in hamster brain using [3H]CP55,940 binding and autoradiography. The FAAH inhibitor URB597 inhibited FAAH activity (IC50 = 12.8 nM) and elevated levels of fatty-acid amides (N-palmitoyl ethanolamine and N-oleoyl ethanolamine) in hamster brain. Anandamide levels were not reliably altered. The cannabinoid agonist WIN55,212-2 (1- 10 mg/kg i.p.) induced CB1-mediated motor ataxia. Blockade of CB1 with rimonabant (5 mg/kg i.p.) induced anxiogenic-like behavior in the elevated plus maze. URB597 (0.1-0.3 mg/kg i.p.) induced CB1-mediated anxiolytic-like effects in the elevated plus maze, similar to the benzodiazepine diazepam (2 mg/kg i.p.). Diazepam (2-6 mg/kg i.p.) suppressed the expression, but not the acquisition, of conditioned defeat. By contrast, neither URB597 (0.3-3.0 mg/kg i.p.) nor rimonabant (5 mg/kg i.p.) altered unconditioned or conditioned social defeat or rota-rod performance.

CONCLUSIONS

Endocannabinoids engage functional CB1 receptors in hamster brain to suppress anxiety-like behavior and undergo enzymatic hydrolysis catalyzed by FAAH. Our results further suggest that neither unconditioned nor conditioned social defeat in the Syrian hamster is dependent upon cannabinoid CB1 receptor activation.

Authors+Show Affiliations

Neuroscience and Behavior Program, Department of Psychology, University of Georgia, Athens, GA 30602-3013, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18545985

Citation

Moise, Anna M., et al. "An Endocannabinoid Signaling System Modulates Anxiety-like Behavior in Male Syrian Hamsters." Psychopharmacology, vol. 200, no. 3, 2008, pp. 333-46.
Moise AM, Eisenstein SA, Astarita G, et al. An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters. Psychopharmacology (Berl). 2008;200(3):333-46.
Moise, A. M., Eisenstein, S. A., Astarita, G., Piomelli, D., & Hohmann, A. G. (2008). An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters. Psychopharmacology, 200(3), 333-46. https://doi.org/10.1007/s00213-008-1209-5
Moise AM, et al. An Endocannabinoid Signaling System Modulates Anxiety-like Behavior in Male Syrian Hamsters. Psychopharmacology (Berl). 2008;200(3):333-46. PubMed PMID: 18545985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters. AU - Moise,Anna M, AU - Eisenstein,Sarah A, AU - Astarita,Giuseppe, AU - Piomelli,Daniele, AU - Hohmann,Andrea G, Y1 - 2008/06/11/ PY - 2007/11/29/received PY - 2008/05/17/accepted PY - 2008/6/12/pubmed PY - 2009/2/10/medline PY - 2008/6/12/entrez SP - 333 EP - 46 JF - Psychopharmacology JO - Psychopharmacology (Berl.) VL - 200 IS - 3 N2 - RATIONALE: An endocannabinoid signaling system has not been identified in hamsters. OBJECTIVE: We examined the existence of an endocannabinoid signaling system in Syrian hamsters using neuroanatomical, biochemical, and behavioral pharmacological approaches. MATERIALS AND METHODS: The distribution of cannabinoid receptors was mapped, and membrane fatty-acid amide hydrolase (FAAH) activity and levels of fatty-acid amides were measured in hamster brain. The impact of cannabinoid CB1 receptor blockade and inhibition of FAAH was evaluated in the elevated plus maze, rota-rod test, and models of unconditioned and conditioned social defeat. RESULTS: A characteristic heterogeneous distribution of cannabinoid receptors was detected in hamster brain using [3H]CP55,940 binding and autoradiography. The FAAH inhibitor URB597 inhibited FAAH activity (IC50 = 12.8 nM) and elevated levels of fatty-acid amides (N-palmitoyl ethanolamine and N-oleoyl ethanolamine) in hamster brain. Anandamide levels were not reliably altered. The cannabinoid agonist WIN55,212-2 (1- 10 mg/kg i.p.) induced CB1-mediated motor ataxia. Blockade of CB1 with rimonabant (5 mg/kg i.p.) induced anxiogenic-like behavior in the elevated plus maze. URB597 (0.1-0.3 mg/kg i.p.) induced CB1-mediated anxiolytic-like effects in the elevated plus maze, similar to the benzodiazepine diazepam (2 mg/kg i.p.). Diazepam (2-6 mg/kg i.p.) suppressed the expression, but not the acquisition, of conditioned defeat. By contrast, neither URB597 (0.3-3.0 mg/kg i.p.) nor rimonabant (5 mg/kg i.p.) altered unconditioned or conditioned social defeat or rota-rod performance. CONCLUSIONS: Endocannabinoids engage functional CB1 receptors in hamster brain to suppress anxiety-like behavior and undergo enzymatic hydrolysis catalyzed by FAAH. Our results further suggest that neither unconditioned nor conditioned social defeat in the Syrian hamster is dependent upon cannabinoid CB1 receptor activation. SN - 0033-3158 UR - https://www.unboundmedicine.com/medline/citation/18545985/An_endocannabinoid_signaling_system_modulates_anxiety_like_behavior_in_male_Syrian_hamsters_ L2 - https://dx.doi.org/10.1007/s00213-008-1209-5 DB - PRIME DP - Unbound Medicine ER -