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Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight /ion trap).
Electrophoresis. 2008 Jul; 29(13):2852-61.E

Abstract

A method for selective extraction using SPE, electrophoretic separation at basic condition and the identification by using exact masses and fragmentation patterns has been developed in order to know the anthocyanins in dried calyces of Hibiscus sabdariffa L. A detailed and comparative study of several extraction procedures has been carried out to obtain the maximum number of anthocyanidins from the calyces and then a CE-TOF-MS method in positive mode using ESI has been developed for the separation and rapid identification of anthocyanins in H. sabdariffa L. Delphinidin-3-sambubioside, cyanidin-3-sambubioside have been detected as main components and cyanidin-3-O-rutinoside, delphinidin-3-O-glucoside and cyanidin-3,5-diglucoside, and chlorogenic acid as minor constituents. The confirmation of the anthocyanidins and chlorogenic acid was carried out using fragmentation ions with the IT-mass spectrometer (IT-MS).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Segura-Carretero A
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain. ansegura@ugr.es
Puertas-Mejía MA
No affiliation info available
Cortacero-Ramírez S
No affiliation info available
Beltrán R
No affiliation info available
Alonso-Villaverde C
No affiliation info available
Joven J
No affiliation info available
Dinelli G
No affiliation info available
Fernández-Gutiérrez A
No affiliation info available

MeSH

AnthocyaninsElectrophoresis, CapillaryFlowersHibiscusSolid Phase ExtractionSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18546170

Citation

Segura-Carretero, Antonio, et al. "Selective Extraction, Separation, and Identification of Anthocyanins From Hibiscus Sabdariffa L. Using Solid Phase Extraction-capillary Electrophoresis-mass Spectrometry (time-of-flight /ion Trap)." Electrophoresis, vol. 29, no. 13, 2008, pp. 2852-61.
Segura-Carretero A, Puertas-Mejía MA, Cortacero-Ramírez S, et al. Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight /ion trap). Electrophoresis. 2008;29(13):2852-61.
Segura-Carretero, A., Puertas-Mejía, M. A., Cortacero-Ramírez, S., Beltrán, R., Alonso-Villaverde, C., Joven, J., Dinelli, G., & Fernández-Gutiérrez, A. (2008). Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight /ion trap). Electrophoresis, 29(13), 2852-61. https://doi.org/10.1002/elps.200700819
Segura-Carretero A, et al. Selective Extraction, Separation, and Identification of Anthocyanins From Hibiscus Sabdariffa L. Using Solid Phase Extraction-capillary Electrophoresis-mass Spectrometry (time-of-flight /ion Trap). Electrophoresis. 2008;29(13):2852-61. PubMed PMID: 18546170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight /ion trap). AU - Segura-Carretero,Antonio, AU - Puertas-Mejía,Miguel A, AU - Cortacero-Ramírez,Sonia, AU - Beltrán,Raúl, AU - Alonso-Villaverde,Carlos, AU - Joven,Jorge, AU - Dinelli,Giovanni, AU - Fernández-Gutiérrez,Alberto, PY - 2008/6/12/pubmed PY - 2008/10/1/medline PY - 2008/6/12/entrez SP - 2852 EP - 61 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 13 N2 - A method for selective extraction using SPE, electrophoretic separation at basic condition and the identification by using exact masses and fragmentation patterns has been developed in order to know the anthocyanins in dried calyces of Hibiscus sabdariffa L. A detailed and comparative study of several extraction procedures has been carried out to obtain the maximum number of anthocyanidins from the calyces and then a CE-TOF-MS method in positive mode using ESI has been developed for the separation and rapid identification of anthocyanins in H. sabdariffa L. Delphinidin-3-sambubioside, cyanidin-3-sambubioside have been detected as main components and cyanidin-3-O-rutinoside, delphinidin-3-O-glucoside and cyanidin-3,5-diglucoside, and chlorogenic acid as minor constituents. The confirmation of the anthocyanidins and chlorogenic acid was carried out using fragmentation ions with the IT-mass spectrometer (IT-MS). SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18546170/Selective_extraction_separation_and_identification_of_anthocyanins_from_Hibiscus_sabdariffa_L__using_solid_phase_extraction_capillary_electrophoresis_mass_spectrometry__time_of_flight_/ion_trap__ L2 - https://doi.org/10.1002/elps.200700819 DB - PRIME DP - Unbound Medicine ER -