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Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) in mice.
Acta Trop. 2008 Aug; 107(2):106-12.AT

Abstract

Although DNA vaccines have several advantages over conventional vaccines, antibody production and protection are often not adequate, particularly in single plasmid vaccine formulation. In the present study we evaluated the efficacy of a cocktail vaccine based on plasmids encoding larval (L3) stage-specific Brugia malayi abundant larval transcript (BmALT-2) and antioxidant detoxification enzyme B. malayi thioredoxin peroxidase (BmTPX) to induce antibodies, protective efficacy and cell-mediated immune response in mice. Mice immunized with cocktail DNA vaccines containing the pVAX ALT-2+TPX developed higher titers of anti-BmALT-2+TPX (1:5000) antibodies, compared to the mice immunized with single DNA vaccine of pVAX ALT-2 or pVAX TPX (1:2000). Correlating with this, the mice administered with cocktail vaccine induced up to 78% of cytotoxicity against B. malayi mf. This cytotoxicity was high compared to 34% induced by the pVAX-ALT2 or 37% by pVAX-TPX. Moreover, cocktail vaccination of mice resulted in significantly higher level of cellular proliferative response associated with raised levels of IFN-gamma that skewed towards Th1 type of response compared to vaccination using either of the components. Taken together, these data suggest that the combination of two or more antigens maybe an effective vaccine development strategy to improve protection and immunogenicity against human lymphatic filariasis.

Authors+Show Affiliations

Centre for Biotechnology, Anna University, Chennai, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18547532

Citation

Anand, Setty Balakrishnan, et al. "Comparison of Immunogenicity, Protective Efficacy of Single and Cocktail DNA Vaccine of Brugia Malayi Abundant Larval Transcript (ALT-2) and Thioredoxin Peroxidase (TPX) in Mice." Acta Tropica, vol. 107, no. 2, 2008, pp. 106-12.
Anand SB, Murugan V, Prabhu PR, et al. Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) in mice. Acta Trop. 2008;107(2):106-12.
Anand, S. B., Murugan, V., Prabhu, P. R., Anandharaman, V., Reddy, M. V., & Kaliraj, P. (2008). Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) in mice. Acta Tropica, 107(2), 106-12. https://doi.org/10.1016/j.actatropica.2008.04.018
Anand SB, et al. Comparison of Immunogenicity, Protective Efficacy of Single and Cocktail DNA Vaccine of Brugia Malayi Abundant Larval Transcript (ALT-2) and Thioredoxin Peroxidase (TPX) in Mice. Acta Trop. 2008;107(2):106-12. PubMed PMID: 18547532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) in mice. AU - Anand,Setty Balakrishnan, AU - Murugan,Vadivel, AU - Prabhu,Prince Rajaiah, AU - Anandharaman,Veerabhadran, AU - Reddy,Maryada Venkata Rami, AU - Kaliraj,Perumal, Y1 - 2008/05/01/ PY - 2007/04/20/received PY - 2008/04/18/revised PY - 2008/04/22/accepted PY - 2008/6/13/pubmed PY - 2008/10/16/medline PY - 2008/6/13/entrez SP - 106 EP - 12 JF - Acta tropica JO - Acta Trop VL - 107 IS - 2 N2 - Although DNA vaccines have several advantages over conventional vaccines, antibody production and protection are often not adequate, particularly in single plasmid vaccine formulation. In the present study we evaluated the efficacy of a cocktail vaccine based on plasmids encoding larval (L3) stage-specific Brugia malayi abundant larval transcript (BmALT-2) and antioxidant detoxification enzyme B. malayi thioredoxin peroxidase (BmTPX) to induce antibodies, protective efficacy and cell-mediated immune response in mice. Mice immunized with cocktail DNA vaccines containing the pVAX ALT-2+TPX developed higher titers of anti-BmALT-2+TPX (1:5000) antibodies, compared to the mice immunized with single DNA vaccine of pVAX ALT-2 or pVAX TPX (1:2000). Correlating with this, the mice administered with cocktail vaccine induced up to 78% of cytotoxicity against B. malayi mf. This cytotoxicity was high compared to 34% induced by the pVAX-ALT2 or 37% by pVAX-TPX. Moreover, cocktail vaccination of mice resulted in significantly higher level of cellular proliferative response associated with raised levels of IFN-gamma that skewed towards Th1 type of response compared to vaccination using either of the components. Taken together, these data suggest that the combination of two or more antigens maybe an effective vaccine development strategy to improve protection and immunogenicity against human lymphatic filariasis. SN - 0001-706X UR - https://www.unboundmedicine.com/medline/citation/18547532/Comparison_of_immunogenicity_protective_efficacy_of_single_and_cocktail_DNA_vaccine_of_Brugia_malayi_abundant_larval_transcript__ALT_2__and_thioredoxin_peroxidase__TPX__in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(08)00109-5 DB - PRIME DP - Unbound Medicine ER -